Preparation method and application of environment estrogen sensor marked by nitrogen-doped graphene

A nitrogen-doped graphene, environmental estrogen technology, applied in the direction of instruments, scientific instruments, material inspection products, etc., to achieve the effect of promoting electron transfer, reducing detection limit, and improving sensitivity

Active Publication Date: 2016-03-30
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many detection methods for environmental estrog

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation method and application of an environmental estrogen sensor based on nitrogen-doped graphene labeling

[0028] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 3 μL, 0.5 mg / mL AuAg heterogeneous alloy solution dropwise to the electrode surface, and drying at room temperature to form a film;

[0029] (2) Add 6 μL of environmental estrogen antibody and 6 μL of bovine serum albumin BSA solution with a mass fraction of 0.5% onto the surface of the electrode successively to seal the non-specific active sites on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C Dry;

[0030] (3) Drop 6 μL, 0.003~5ng / mL of a series of environmental estrogen antigen standard solutions of different concentrations onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0031] (4) Add 6 μL nitrogen-dope...

Embodiment 2

[0033] Preparation method and application of an environmental estrogen sensor based on nitrogen-doped graphene labeling

[0034] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 5 μL, 1mg / mL AuAg heterogeneous alloy solution dropwise to the electrode surface, and drying at room temperature to form a film;

[0035] (2) Add 6 μL of environmental estrogen antibody and 6 μL of bovine serum albumin BSA solution with a mass fraction of 1% successively on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C Dry;

[0036] (3) Drop 6 μL, 0.003~5ng / mL of a series of environmental estrogen antigen standard solutions of different concentrations onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0037] (4) Add 6 μL nitrogen-doped graph...

Embodiment 3

[0039] Preparation method and application of an environmental estrogen sensor based on nitrogen-doped graphene labeling

[0040] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 8μL, 2mg / mL AuAg heterogeneous alloy liquid onto the electrode surface, and drying at room temperature to form a film;

[0041] (2) Add 6 μL of environmental estrogen antibody and 6 μL of bovine serum albumin BSA solution with a mass fraction of 2.5% on the surface of the electrode successively to seal the non-specific active sites on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C Dry;

[0042] (3) Drop 6 μL, 0.003~5ng / mL of a series of environmental estrogen antigen standard solutions of different concentrations onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0043] (4) Add 6 μL nitrogen-doped graphene and ...

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Abstract

The invention relates to a preparation method and application of an environment estrogen sensor marked by nitrogen-doped graphene and belongs to the technical field of novel functional materials and biological sensing detection. The sensor takes an Au@Ag heterogeneous alloy as a substrate material and the nitrogen-doped graphene as a detection antibody decorative material; and by utilizing a layer-by-layer nano self-assembling technology, a sandwiched immunosensor is constructed. By using the synergistic catalysis effect of the Au@Ag heterogeneous alloy and the nitrogen-doped graphene, the sensitivity of the environment estrogen sensor is remarkably improved and the linear range is expanded, so that the preparation method and application of the environment estrogen sensor marked by the nitrogen-doped graphene have very important meanings on detection and analysis of environment estrogens.

Description

technical field [0001] The invention relates to a preparation method and application of an environmental estrogen sensor based on nitrogen-doped graphene labeling. N-GS is used as a marker for detecting antibodies, and AuAg heterogeneous alloy is used as a base material to prepare a sensor for detecting environmental estrogen. An electrochemical immunosensor belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. [0003] At present, there are many detection methods for environmental estrogen, but most of them require large-scale equipment and professional operators. Immunosensor is a biosensor that c...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N27/327
CPCG01N27/3275G01N33/74
Inventor 任祥魏琴庞雪辉马洪敏吴丹张勇孙旭范大伟
Owner UNIV OF JINAN
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