Preparation method and application of environment estrogen sensor marked by nitrogen-doped graphene
A nitrogen-doped graphene, environmental estrogen technology, applied in the direction of instruments, scientific instruments, material inspection products, etc., to achieve the effect of promoting electron transfer, reducing detection limit, and improving sensitivity
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Embodiment 1
[0027] Preparation method and application of an environmental estrogen sensor based on nitrogen-doped graphene labeling
[0028] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 3 μL, 0.5 mg / mL AuAg heterogeneous alloy solution dropwise to the electrode surface, and drying at room temperature to form a film;
[0029] (2) Add 6 μL of environmental estrogen antibody and 6 μL of bovine serum albumin BSA solution with a mass fraction of 0.5% onto the surface of the electrode successively to seal the non-specific active sites on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C Dry;
[0030] (3) Drop 6 μL, 0.003~5ng / mL of a series of environmental estrogen antigen standard solutions of different concentrations onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;
[0031] (4) Add 6 μL nitrogen-dope...
Embodiment 2
[0033] Preparation method and application of an environmental estrogen sensor based on nitrogen-doped graphene labeling
[0034] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 5 μL, 1mg / mL AuAg heterogeneous alloy solution dropwise to the electrode surface, and drying at room temperature to form a film;
[0035] (2) Add 6 μL of environmental estrogen antibody and 6 μL of bovine serum albumin BSA solution with a mass fraction of 1% successively on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C Dry;
[0036] (3) Drop 6 μL, 0.003~5ng / mL of a series of environmental estrogen antigen standard solutions of different concentrations onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;
[0037] (4) Add 6 μL nitrogen-doped graph...
Embodiment 3
[0039] Preparation method and application of an environmental estrogen sensor based on nitrogen-doped graphene labeling
[0040] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water; adding 8μL, 2mg / mL AuAg heterogeneous alloy liquid onto the electrode surface, and drying at room temperature to form a film;
[0041] (2) Add 6 μL of environmental estrogen antibody and 6 μL of bovine serum albumin BSA solution with a mass fraction of 2.5% on the surface of the electrode successively to seal the non-specific active sites on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C Dry;
[0042] (3) Drop 6 μL, 0.003~5ng / mL of a series of environmental estrogen antigen standard solutions of different concentrations onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;
[0043] (4) Add 6 μL nitrogen-doped graphene and ...
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