Method for determining components and estimating anti-gen content of foot-and-mouth disease vaccine

Abstract

The invention provides a method for determining components and estimating the anti-gen content of a foot-and-mouth disease vaccine. The method comprises the following steps: (1) performing emulsion resolving on a foot-and-mouth disease vaccine to separate out an antigen phase; (2) concentrating the antigen phase; (3) using purified foot-and-mouth disease virus Asia 1-type, O-type and A-type 146S antigens to immunize animals and prepare detection serums; (4) diluting the concentrated antigens by different multiples and respectively performing immunodiffusion tests on the detection serums; (5) judging the components of the foot-and-mouth disease vaccine according to precipitation lines, that is, whether the vaccine is an O-type univalent vaccine, an O-type + A-type bivalent vaccine or an O-type + Asia 1-type + A-type trivalent vaccine; (6) estimating the content of the antigen in a detected vaccine sample according to the highest antigen dilution multiple at which the precipitation lines appear. The method can not only identify the components of the foot-and-mouth disease vaccine but also estimate the contents of all the components, and is simple to operate, strong in specificity, good in stability and suitable for basic-level application.

Description

technical field [0001] The invention relates to a method for determining the components of the foot-and-mouth disease vaccine and estimating the antigen content. Background technique [0002] Foot and mouth disease (foot and mouth disease, FMD) is an acute, highly contagious infectious disease common to pigs, cattle, sheep and other main livestock and other cloven-hoofed animals. The clinical feature is the development of blisters on the oral mucosa, hooves, and breast skin. The disease has many transmission routes and fast speed, and has repeatedly broken out in the world, causing huge political and economic losses. The World Organization for Animal Health (OIE) lists it as an infectious disease that must be declared, and my country also lists it as the first class of animal infectious diseases. [0003] Foot and mouth disease virus (foot and mouth disease virus, FMDV) belongs to picornaviridae foot and mouth disease virus genus, there are seven serotypes: O type, A type,...

AI Technical Summary

Problems solved by technology

The antibody titer test of vaccine immune serum requires the use of non-foot-and-mouth disease vaccines to immunize animals, which cannot fundamentally replace the use of this animal, and there are still difficulties in the selection of animals

Moreover, these two methods are "after-the-fact evaluation", and the quality of the vaccine cannot be evaluated before the vaccine is used, and unqualified products can be eliminated.

Moreover, these two methods have high requirements on the laboratory. The virus neutralization test must be carried out in a high-safety laboratory to prevent the virus from spreading out; ELISA must have a constant temperature incubator, microplate reader, etc., which is not suitable for grassroots applications.

Images