A kind of Burkholderia pyrrole and its application in promoting the growth of A.

A technology of Burkholderia pyrrole and Holderia, which is applied in the fields of application, plant growth regulator, plant growth regulator, etc., can solve the problems such as no growth-promoting bacteria of A. Effects of strain resources, promotion of growth and development, and strong auxin secretion

Active Publication Date: 2018-11-09
长治学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no reports on the growth-promoting bacteria of Lianxiang tree at home and abroad

Method used

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  • A kind of Burkholderia pyrrole and its application in promoting the growth of A.
  • A kind of Burkholderia pyrrole and its application in promoting the growth of A.
  • A kind of Burkholderia pyrrole and its application in promoting the growth of A.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Determination of the ability of LWK2 liquid to dissolve potassium.

[0027] Potassium-solubilizing ability assay medium: sucrose 5g, MgSO 4 ·7H 2 O 0.5g, Na 2 HPO 4 2g, potassium aluminosilicate 2g, FeCl 3 0.005g, pH7.0~7.5, tap water 1000mL, sterilize at 115℃ for 30min.

[0028] Inoculate a single colony of LWK2 after activation in LB (tryptone 10g, yeast extract 5g, sodium chloride 10g, deionized water 1000mL, pH 7.0, sterilize at 121°C for 20min) liquid medium, shake culture at 30°C for 18 ~24h, fully shake to form a uniform bacterial suspension, as the seed liquid. Take 0.5mL and inoculate it into a 300mL Erlenmeyer flask containing 50mL of potassium-dissolving capacity test medium, and inoculate an equal amount of LB liquid medium with the potassium-dissolving capacity test medium as a blank control. Incubate at 28°C with shaking at 200r / min for 5 days. The fermented liquid obtained is placed in a water bath and concentrated to about 10 mL, and 6...

Embodiment 2

[0030] Example 2: Determination of the ability of LWK2 to produce auxin.

[0031] King's B medium: peptone 20g, MgSO 4 ·7H 2 O 1.5g, K 2 HPO 4 1.5g, glycerol 10mL, agar 15g, after adjusting the pH value to 7.0, add to 1000mL.

[0032] S1 colorimetric solution: weigh 12g FeCl 3 Dissolve in 300mL deionized water, slowly add 429.7mL concentrated sulfuric acid, and dilute to 1L after cooling.

[0033] Prepare IAA standard solutions with concentrations of 1, 4, 6, 8, 10, 12, 14, 16, 18, and 20 mg / L, and mix them with S1 reagent at a volume ratio of 1:1, and place them in the dark at room temperature for 30 minutes, then The OD530 values ​​of each concentration were measured respectively (the 1:1 mixture of distilled water and S1 reagent was used as the blank control) value. Taking the IAA concentration as the abscissa and OD530 as the ordinate, the standard curve of IAA is obtained.

[0034] Inoculate a single colony of LWK2 in NB liquid medium and culture for 12h (28°C, 12...

Embodiment 3

[0036] Example 3: Determination of the ability of LWK2 to promote growth of cucumber seeds.

[0037] Cucumber seeds were treated with 10% hydrogen peroxide for 20 minutes, rinsed with sterile water 5-6 times, and dried for later use.

[0038] Inoculate a single colony of the LWK2 strain in NB liquid medium, and culture it on a shaker at 28°C for 24-36 hours, so that the bacterial concentration reaches 10 9 cfu / mL. The bacterial suspension was serially diluted to 10 -7 , divide the bottom of the sterile petri dish into 8 equal areas with a marker pen, mark CK, -1, -2, -3, -4, -5, -6, -7 in turn, place 4 in each area ~6 pieces of sterile filter paper, put sterile absorbent cotton in the middle, put the processed cucumber seeds on the filter paper, drop 100 μl of LWK2 bacterial suspension corresponding to the dilution in the center of the filter paper in each area of ​​the bottom of the dish, and CK area An equal amount of sterile water was added dropwise as a control. 28 ℃, ...

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PUM

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Abstract

The invention discloses a kind of Burkholderia pyrrocinia, which is classified as Burkholderia pyrrocinia (Burkholderia pyrrocinia), and the strain number is LWK2, which has been preserved in the China Type Culture Collection Center CCTCC, and the preservation number is : CCTCC NO: M 2016007, date of deposit: January 5, 2016. The invention also discloses the application of the above-mentioned Burkholderia pyrrole in promoting the growth of the japonica. The LWK2 strain has strong potassium-decomposing ability and auxin secretion ability; it can obviously promote the germination of cucumber seeds; the LWK2 bacterial agent was inoculated with the seedlings of Lianxiang tree, and the results showed that the bacterial agent can obviously promote the growth of Lianxiang tree seedlings. At the same time, the strain has a certain antagonistic effect on plant pathogens such as rice blast fungus, ascosporium chrysogenum, Fusarium oxysporum, rice sheath blight fungus and rhizoctonia.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a Burkholderia pyrrole and its application in promoting the growth of syringa. Background technique [0002] Cercidiphyllum japonicum Sieb.Et Zucc. is a deciduous tree belonging to Cercidiphyllum japonicum Sieb.Et Zucc., also known as Cercidiphyllum japonicum Sieb.Et Zucc. Because of its aromatic taste, it can be heard from a hundred meters, and the more you shake it, the more fragrant it becomes, so it is named "Lianxiang" (Fu Liguo, 1992). Lianxiang tree is one of the only three plants in Lianxiang family. It is an ancient and rare precious deciduous tall tree, which is intermittently distributed in China and Japan. It was widely distributed in the northern hemisphere during the Cretaceous and Tertiary periods. Since the late Quaternary glacial period, the distribution area has shrunk sharply, and it is now scatt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 晋婷婷白变霞任嘉红陈艳彬李世宏张鹏飞
Owner 长治学院
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