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Method for extracting microalgae oil through compound enzyme

A technology of microalgae oil and compound enzyme, applied in fermentation and other directions, can solve the problems of high pollution, low extraction rate, high energy consumption, etc., and achieve the effects of high environmental safety factor, large sample processing capacity, and low energy consumption

Active Publication Date: 2016-04-20
SHANDONG TAIDE NEW ENERGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of high pollution, high energy consumption and low extraction rate in the existing algae oil extraction method, the present invention provides a method for extracting algae oil with compound enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Select chlorella cultured for 10 days, centrifuge at 5000rpm for 5 minutes, remove the supernatant to obtain algae mud, add water and oscillate to suspend until the weight ratio of the algae mud reaches 30%; add surfactant sodium dodecylsulfonate to the algae mud 0.1%, shake in 100W ultrasonic for 5min, add helicase: cellulase: trypsin ratio of 3:1:1 compound enzyme 0.5%, adjust pH5.5, control temperature at 35℃, keep warm for 5h, finally boil 5 min to inactivate the enzyme. After the enzymolysis solution is cooled, add chloroform:methanol as the extraction agent of 1:1, choose constant temperature oscillation at 30°C for 10 hours, then centrifuge at 5000rpm for 5 minutes, remove the oil layer and distill it to obtain microalgae oil. According to calculation, the oil extraction rate is 92.7% .

Embodiment 2

[0019] Select chlorella cultured for 20 days, centrifuge at 8000rpm for 10 minutes, remove the supernatant to obtain algae mud, add water and shake and suspend until the weight ratio of algae mud reaches 50%; add surfactant sodium dodecylsulfonate to the algae mud 1%, shake in 300W ultrasonic wave for 15min, add helicase: cellulase: trypsin ratio of 5:1:2 compound enzyme 5%, adjust pH8.5, control temperature at 60℃, keep warm for 15h, and finally boil 10 min to inactivate the enzyme. After the enzymolysis solution is cooled, add chloroform:methanol as an extraction agent of 3:1, choose constant temperature oscillation at 50°C for 30 hours, then centrifuge at 8000rpm for 10 minutes, remove the oil layer and distill it to obtain microalgae oil. According to calculation, the oil extraction rate is 93.6% .

Embodiment 3

[0021] Select chlorella cultured for 15 days, centrifuge at 6000rpm for 7 minutes, remove the supernatant to obtain algae mud, add water and shake and suspend until the weight ratio of algae mud reaches 40%; add surfactant sodium dodecylsulfonate to the algae mud 12%, shake in 200W ultrasonic wave for 10min, add helicase: cellulase: trypsin ratio of 4:1:1 compound enzyme 3%, adjust pH6, control temperature at 45°C, keep warm for 10h, and finally boil for 7min. Enzyme inactivation. After the enzymolysis solution is cooled, add chloroform:methanol as 2:1 extractant, choose constant temperature oscillation at 40°C for 20 hours, then centrifuge at 6000rpm for 7 minutes, remove the oil layer and distill it to obtain microalgae oil. According to calculation, the oil extraction rate is 98.3% .

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Abstract

The invention discloses a method for extracting microalgae oil through a compound enzyme. The method comprises the steps that the compound enzyme composed of helicase, cellulase and trypsin and surfactant are added to suspended algae slurry subjected to ultrasonic oscillation, microalgae cells are broken through enzymolysis to release oil, extraction is conducted through an extraction agent composed of chloroform methanol, and the oil extraction rate reaches 90% or above. According to the method for extracting the microalgae oil through the compound enzyme, the reaction condition is mild, energy consumption is low, the processing cost is low, and the method is suitable for large-scale industrial production of algae oil.

Description

technical field [0001] The invention belongs to the field of biological energy, and in particular relates to a method for extracting oil from microalgae with compound enzymes. Background technique [0002] With the growing contradiction between population growth and natural resource shortage, the energy crisis is getting worse, and the original fossil fuels can no longer meet the daily needs of human beings. The development of new bio-energy has become the focus of global attention. Among them, the development of biodiesel based on renewable oils and fats has attracted much attention. Biodiesel is a fatty acid monoalkyl ester obtained by the transesterification reaction of bio-oil (fatty acid triglyceride) and alcohol (methanol or ethanol). Its physical properties are close to those of petrochemical diesel. High hexane number, good lubricity. Microalgae oil is an important source of biological oil, which can reach 20-75% of the dry weight of cells. The main components are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/64
Inventor 车春玲
Owner SHANDONG TAIDE NEW ENERGY
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