Method for measuring soil amino acid nutrition contribution

A technology of nutrient contribution and amino acids, applied in the field of soil science, can solve problems such as difficult long-term experiments, affecting calculation accuracy, confusing molecular amino acid absorption, etc., and achieves the effect of simple operation and accurate and reliable data.

Active Publication Date: 2016-04-20
ZHEJIANG UNIV
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Problems solved by technology

This method provides an important basis for the quantitative study of the bioavailability of amino acids, but it also has important defects: 1) After microorganisms decompose the labeled amino acids, 13 C may still enter the plant in other ways, which confuses the absorption of molecular amino acids; 2) the absorption of plant roots 13 C will be excreted through re

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  • Method for measuring soil amino acid nutrition contribution
  • Method for measuring soil amino acid nutrition contribution
  • Method for measuring soil amino acid nutrition contribution

Examples

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Embodiment 1

[0027] (1) Soil preparation: collect the 0-15cm surface soil in the west area of ​​Zijingang, Zhejiang University, take it back and pass it through a 4mm sieve, remove obvious stones and roots, pass it through a 2mm sieve after natural air drying in a cool and ventilated place, and bag it for later use;

[0028] (2) Plant planting: Take 2000g of the above-mentioned prepared soil in plastic pots with a height and a diameter of 17cm and 12.5cm respectively. The bottom of the plastic pot does not contain permeable holes. A polyethylene pipe full of small holes is used to plant germinated corn seeds in the center of a plastic pot, and when the corn grows to a height of 2 cm (the corn has germinated for 4-8 days), select the consistent corn seedlings;

[0029] There are no permeable holes at the bottom of the plastic basin, which can avoid the loss of markers added later. Inserting polyethylene pipes can make the soil better aerated, the root system grows evenly, and it is conducive...

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Abstract

The invention provides a method for measuring soil amino acid nutrition contribution. The method is a method for piecewise fitting by calculus and for quantitative evaluation of amino acid nutrition contribution to plant growth under the microbe existence condition. By combining carbon-nitrogen double labeling technique and principle of calculus, long-term nutrition contribution of amino acid to plant growth is studied in the environment of considering microbiological effect. Thus, the invention provides a method basis for the research on organotrophy. The method is simple to operate, and the culture environment is easy to regulate and control. By the isotope technique, data is accurate and reliable and has high accuracy. Amino acid nutrition contribution to various plants can be studied. Theoretical and technical supports are provided for efficient management of nutrient resources.

Description

technical field [0001] This note is in the field of soil science and relates to a method for determining the nutrient contribution of soil amino acids. Background technique [0002] In the past decades of plant nutrition research, inorganic nitrogen nutrition has been intensively and extensively studied, while research on organic nitrogen nutrition is relatively lacking. However, the widespread application of inorganic nitrogen, especially chemical nitrogen fertilizers, while increasing crop yields, has also brought about a series of problems such as the decline in the quality of agricultural products, environmental pollution, poor plant disease resistance, and pesticide residues. With the continuous development of organic agriculture and organic food, plant organic nutrition is getting more and more attention. Studies in recent years have found that plants can not only absorb inorganic molecules such as ammonium nitrogen and nitrate nitrogen, but also absorb simple or comp...

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Application Information

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IPC IPC(8): G01N31/00G01N27/62
CPCG01N27/62G01N31/002
Inventor 马庆旭吴良欢
Owner ZHEJIANG UNIV
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