Method for measuring soil amino acid nutrition contribution

Abstract

The invention provides a method for measuring soil amino acid nutrition contribution. The method is a method for piecewise fitting by calculus and for quantitative evaluation of amino acid nutrition contribution to plant growth under the microbe existence condition. By combining carbon-nitrogen double labeling technique and principle of calculus, long-term nutrition contribution of amino acid to plant growth is studied in the environment of considering microbiological effect. Thus, the invention provides a method basis for the research on organotrophy. The method is simple to operate, and the culture environment is easy to regulate and control. By the isotope technique, data is accurate and reliable and has high accuracy. Amino acid nutrition contribution to various plants can be studied. Theoretical and technical supports are provided for efficient management of nutrient resources.

Description

technical field [0001] This note is in the field of soil science and relates to a method for determining the nutrient contribution of soil amino acids. Background technique [0002] In the past decades of plant nutrition research, inorganic nitrogen nutrition has been intensively and extensively studied, while research on organic nitrogen nutrition is relatively lacking. However, the widespread application of inorganic nitrogen, especially chemical nitrogen fertilizers, while increasing crop yields, has also brought about a series of problems such as the decline in the quality of agricultural products, environmental pollution, poor plant disease resistance, and pesticide residues. With the continuous development of organic agriculture and organic food, plant organic nutrition is getting more and more attention. Studies in recent years have found that plants can not only absorb inorganic molecules such as ammonium nitrogen and nitrate nitrogen, but also absorb simple or comp...

AI Technical Summary

Problems solved by technology

This method provides an important basis for the quantitative study of the bioavailability of amino acids, but it also has important defects: 1) After microorganisms decompose the labeled amino acids, 13 C may still enter the plant in other ways, which confuses the absorption of molecular amino acids; 2) the absorption of plant roots 13 C will be excreted through respiration, which affects the accuracy of calculation; 3) The marker substance will be rapidly decomposed by microorganisms, making it difficult to carry out long-term tests

All in all, the double-labeling technique is only suitable for short-term culture experiments, and it is difficult to evaluate the bioavailability of amino acids in the natural state for a long time

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