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Thyroglobulin (TG) test kit and test method thereof

A technology of thyroglobulin and kits, applied in biological testing, measuring devices, material inspection products, etc.

Inactive Publication Date: 2016-04-20
BEIJING HOMA BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have many advantages, they still need to be further improved in terms of detection sensitivity, specificity, stability, and detection time.

Method used

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  • Thyroglobulin (TG) test kit and test method thereof
  • Thyroglobulin (TG) test kit and test method thereof
  • Thyroglobulin (TG) test kit and test method thereof

Examples

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Effect test

Embodiment 1

[0089] A test kit for thyroglobulin, which consists of magnetic separation reagents, reagent R 1 , reagent R 2 , standard product, quality control product, calibrator, cleaning concentrate, diluent and luminescent substrate composition, wherein: magnetic separation reagent: nano magnetic microspheres labeled with mouse anti-human TG monoclonal antibody, said label has The concentration of nano-magnetic microspheres of mouse anti-human TG monoclonal antibody is 167 μg / ml; reagent R 1 : Containing alkaline phosphatase-labeled TG antibody, the concentration of the alkaline phosphatase-labeled TG antibody is 0.3 μg / ml; reagent R 2 : buffer solution containing bovine gamma globulin protein components; standard, quality control and calibrator: calf serum solution containing a certain amount of TG antigen, the concentration of the standard is 0 (S0), 2 (S1), 10(S2), 100(S3), 250(S4), 500(S5)ng / ml, the concentration of the quality control substance is 6,250ng / ml, and the concentrati...

Embodiment 2

[0178] A test kit for thyroglobulin, which consists of magnetic separation reagents, reagent R 1 , reagent R 2 , standard product, quality control product, calibrator, cleaning concentrate, diluent and luminescent substrate composition, wherein: magnetic separation reagent: nano magnetic microspheres labeled with mouse anti-human TG monoclonal antibody, said label has The concentration of nano-magnetic microspheres of mouse anti-human TG monoclonal antibody is 167 μg / ml; reagent R 1 : Containing alkaline phosphatase-labeled TG antibody, the concentration of the alkaline phosphatase-labeled TG antibody is 0.3 μg / ml; reagent R 2 : buffer solution containing bovine gamma globulin protein components; standard, quality control and calibrator: calf serum solution containing a certain amount of TG antigen, the concentration of the standard is 0 (S0), 2 (S1), 10(S2), 100(S3), 250(S4), 500(S5)ng / ml, the concentration of the quality control substance is 6,250ng / ml, and the concentrati...

Embodiment 3

[0194] A test kit for thyroglobulin, which consists of magnetic separation reagents, reagent R 1 , reagent R 2 , standard product, quality control product, calibrator, cleaning concentrate, diluent and luminescent substrate composition, wherein: magnetic separation reagent: nano magnetic microspheres labeled with mouse anti-human TG monoclonal antibody, said label has The concentration of nano-magnetic microspheres of mouse anti-human TG monoclonal antibody is 167 μg / ml; reagent R 1 : Containing alkaline phosphatase-labeled TG antibody, the concentration of the alkaline phosphatase-labeled TG antibody is 0.3 μg / ml; reagent R 2 : buffer solution containing bovine gamma globulin protein components; standard, quality control and calibrator: calf serum solution containing a certain amount of TG antigen, the concentration of the standard is 0 (S0), 2 (S1), 10(S2), 100(S3), 250(S4), 500(S5)ng / ml, the concentration of the quality control substance is 6,250ng / ml, and the concentrati...

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Abstract

The invention discloses a thyroglobulin (TG) test kit, which is composed of a magnetic separation reagent, a reagent R1, a reagent R2, a standard substance, a quality control substance, a calibration substance, a cleaning concentrated solution, a dilution solution and a luminous substrate. The invention also discloses a preparation method of the kit. The kit provided by the invention combines the chemiluminescence technique and immune magnetic particles, and provides a reaction system close to a homogeneous phase. Compared with existing ELISA (enzyme-linked immuno sorbent assay) techniques, the kit and the method provided by the invention have the advantages of higher specificity and sensitivity, short detection time, simple operation mode, and accurate and reliable detection result, and greatly lower the product cost.

Description

technical field [0001] The invention relates to a kit for measuring serum and a testing method thereof, in particular to a kit for testing thyroglobulin content in serum and a testing method thereof. Background technique [0002] Thyroglobulin (TG) is a 660ku glycoprotein secreted by the thyroid follicular epithelium. Each TG contains about 2 thyroxine (T4) and 0.5 triiodothyronine (T3) molecules, stored in the filter In the bubble cavity. Lysosomes hydrolyze T4 and T3 on the surface of TG and release them into the blood, and a small amount of TG is also released into the blood, and part of TG is secreted into the blood through the thyroid lymphatic vessels. Circulating TG is cleared by macrophages in the liver. Changes in serum TG are more common in malignant tumors of the thyroid. For example, TG can be elevated to varying degrees in thyroid follicular carcinoma, thyroid papillary carcinoma, and anaplastic carcinoma; while blood TG in medullary thyroid carcinoma can be n...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/6854G01N33/54326
Inventor 朱驰王键
Owner BEIJING HOMA BIOLOGICAL ENG
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