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Node order use method for high-concentration NAA and glycerin gel

A glycerin gel, high-concentration technology, applied in the field of plant biology, can solve problems such as high cost, loss of branches, death, etc., and achieve the effect of easy operation and reduced water evaporation

Inactive Publication Date: 2016-04-27
何晴雪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] ① Hormone mother solution preparation: Accurately weigh 0.1g of NAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL NAA mother solution;

[0014] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:2 into a high-concentration hormone working solution with a concentration of 500mg / L, and add 10ml of glycerin to 90ml of hormone working solution at the same time;

[0015] ③ Hormone gel preparation: Accurately weigh 1g of sodium carboxymethylcellulose and 8g of sodium alginate, and dissolve them in 100mL of hormone and glycerin working solution with a concentration of 500mg / L in a water bath at 50°C for later use;

[0016] Cutting: Insert the calendula branch into the cultivation medium;

[0017] Dropping at the axillary buds: Add the gel sealant dropwise to the axillary buds of the branches.

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PUM

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Abstract

The invention discloses a node order use method for high-concentration NAA and glycerin gel and belongs to the technical field of plant biology. The method comprises the steps: firstly, preparing 1mg / mL NAA mother liquor; diluting the mother liquor, so as to form 500mg / L high-concentration NAA working fluid; preparing high-concentration NAA and glycerin gel confining liquid, of which the mass concentration of sodium carboxymethyl cellulose is 1% and the mass concentration of sodium alginate is 8%, by using the working fluid; inserting branches into a culture substrate; and dropwise adding the gel confining liquid to axillary buds of healthy and robust branches.

Description

technical field [0001] The invention discloses a node-site application method of high-concentration NAA and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as NAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vi...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06
CPCA01G7/06A01G22/00
Inventor 何晴雪
Owner 何晴雪