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Hybrid paper mulberry industrial tissue culture and breeding method

A technology of tissue culture and industrialization, which is applied in the field of agriculture, can solve the problems of long time for tissue culture of hybrid mulberry trees, general treatment of mulberry tree seedlings, and high production costs, and achieve the effects of shortening tissue culture time, low production costs, and improving quality

Inactive Publication Date: 2016-05-04
TIANSHUI DENONG SUPPLY & SEED IND DEV CO LTD
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AI Technical Summary

Problems solved by technology

[0010] The existing hybrid mulberry tissue culture takes a long time, the treatment of mulberry seedlings is general, and the proliferation is slow; the production cost is high

Method used

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Experimental program
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Effect test

Embodiment Construction

[0021] This specific embodiment adopts following technical scheme: it comprises the following steps:

[0022] 1. Collection of explants: The collection of tissue culture explants of syringa seedlings is the new shoots with axillary buds. The explants collected in the field or in the field are contaminated with many bacteria and need to be further processed in the laboratory.

[0023] 2. Cleaning and disinfection of explants: The explants collected outdoors have dust and dirt, washed with running water for 30 minutes, soaked in 1% washing water for 5 minutes, disinfectant has 75% ethanol, disinfected for 30 seconds, no Rinse with sterile water 4-5 times; then put in 0.1% mercuric chloride solution, disinfect for 8 minutes and 30 seconds, rinse with sterile water 5-6 times, put into ultra-clean bench, and irradiate with ultraviolet light for 30 minutes.

[0024] 3. Primary culture of explants: After disinfection treatment, the plant tissue becomes non-toxic tissue, cut off the n...

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Abstract

The invention discloses a hybrid paper mulberry industrial tissue culture and breeding method, and belongs to the technical field of agriculture. The method comprises the following steps: collecting explants: collecting tissue culture explants of paper mulberry seedling, wherein the explants are newly born twigs with axillary buds, the explants collected in wild or fields contains many infectious microbes, and thus the explants should be processed in the lab; subjecting explants to primary culture; carrying out multiplication subculture; performing virus seedling rooting culture; exercising seedling: grafting Chinese rose Daziguang tissue culture seedling, which has been subjected to strong seedling subculture, into soil, which has been sterilized and contains vermiculite and river sand, covering the upper layer by a plastic film to retain water to prevent death of seedlings; and planting the seedlings, which survive in the seedling exercise step, in fields. The provided method can effectively shorten the tissue culture time of paper mulberry seedling and improve the quality of paper mulberry tissue culture seedlings; the proliferation is fast, the tissue culture seedling is strong, the rooting is quick, the production cost is low, and the time between explant grafting to seedling is short.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for industrialized tissue culture of hybrid mulberry trees. Background technique [0002] The hybrid mulberry tree is a high-quality tree species that can be planted in most parts of China after ten years of painstaking research and demonstration verification by the scientific and technological personnel of the Chinese Academy of Sciences. Using modern agricultural breeding technology, we have bred Zhongke No. 1, No. 2 and No. 3 hybrid tree species through space-borne breeding and hybrid breeding. [0003] The Institute of Botany, Chinese Academy of Sciences has completed the following research on the hybrid tree: [0004] 1. Through the research on the salt-tolerant biology of different genotypes of mulberry trees, including growth characteristics, ecological characteristics and greening and horticultural traits, etc., breeding materials and strains of mulberry t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 靳杏子马云霞何平礼
Owner TIANSHUI DENONG SUPPLY & SEED IND DEV CO LTD
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