Material of support for treating femoral head necrosis and preparation method thereof
A femoral head necrosis and scaffold material technology, which is applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of osteoarthritis joint function, femoral head collapse, damage and other problems, and achieves good biocompatibility, no cytotoxic effect
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Embodiment 1
[0044] Preparation method of scaffold material for treating femoral head necrosis:
[0045] ① Preparation of Antler Gum
[0046] Crush the deer antler slices and place them in a round-bottomed flask, add 4-5 times (volume) of distilled water, and boil in water; take the juice every 3 hours, add water and cook until the deer antlers are soft and can be crushed by hand; The gum juice was combined, filtered to remove the residue, and concentrated to 1.2g / ml under reduced pressure with a vacuum concentrator at 50°C; cooled to room temperature to obtain the deerhorn gum solution, sterilized by radiation, and stored at 4°C.
[0047] ② Preparation of deproteinized bone
[0048] Take the cancellous bone at the lower end of the pig femur, soak it in normal saline for 24 hours, wash it with distilled water after taking it out, and put it in a closable container with 30% (v / v) H 2 O 2 Place the solution in a glass bottle in a constant temperature water bath at 37°C for 48 hours, and replace the ...
Embodiment 2
[0054] Preparation method of scaffold material for treating femoral head necrosis:
[0055] ① Preparation of Antler Gum
[0056] Crush the deer antler pieces and place them in a round-bottomed flask, add 4–5 times (volume) of distilled water, and boil; take the juice every 3 hours, add water and cook until the deer antlers are soft and soft, and can be crushed by hand; The gum juice is filtered to remove the residue, and concentrated to 1.0g / ml under reduced pressure with a vacuum concentrator at 50°C; the deerhorn gum solution is obtained after cooling to room temperature, sterilized by irradiation, and stored at 4°C.
[0057] ② Preparation of deproteinized bone
[0058] Take the cancellous bone at the lower end of the pig femur, soak it in normal saline for 24 hours, wash it with distilled water after taking it out, and put it in a closable container with 30% (v / v) H 2 O 2 Place the solution in a glass bottle in a constant temperature water bath at 37°C for 48 hours, and replace the...
Embodiment 3
[0064] Preparation method of scaffold material for treating femoral head necrosis:
[0065] ① Preparation of Antler Gum
[0066] Crush the deer antler pieces and place them in a round-bottomed flask, add 4–5 times (volume) of distilled water, and boil; take the juice every 3 hours, add water and cook until the deer antlers are soft and soft, and can be crushed by hand; The glue juice is filtered to remove the residue, and concentrated to 1.33g / ml under reduced pressure with a vacuum concentrator at 50°C; the deerhorn gum solution is obtained after cooling to room temperature, sterilized by irradiation, and stored at 4°C.
[0067] ② Preparation of deproteinized bone
[0068] Take the cancellous bone at the lower end of the pig femur, soak it in normal saline for 24 hours, wash it with distilled water after taking it out, and put it in a closable container with 30% (v / v) H 2 O 2 Place the solution in a glass bottle in a constant temperature water bath at 37°C for 48 hours, and replace t...
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