Microfluidic chip and its application in pesticide detection
A microfluidic chip and pesticide technology, applied in the fields of environmental detection and food safety, can solve problems such as poor flexibility, complicated actual operation, and disadvantageous simultaneous detection of multiple samples, and achieve lower detection limit, low price, and good application prospects. Effect
Active Publication Date: 2017-09-29
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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Problems solved by technology
There have been some reports on building a microfluidic chip platform for pesticide detection. The design of these chips does not give full play to the advantages of disc chips, and the actual operation is complicated, which is not conducive to the simultaneous detection of multiple samples, and the chip has a narrow scope of application and is flexible. poor sex
There has not been a substantial breakthrough in the application of high-resolution and high-sensitivity pesticide detection on chips
Method used
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Embodiment Construction
[0030] In this embodiment, there are microstructures and microchannels on the surface of the disc microfluidic PMMA chip, which can be driven by the centrifugal force generated by the rotation of the centrifuge or by a syringe pump to realize the extraction of pesticides in the sample to be tested and the mixing of various reagents 1. In the reaction process, the content of pesticide residues on the chip is quantitatively detected by a fluorescence spectrophotometer. The microfluidic chip and method can reduce the number of injections and detect multiple samples at the same time, and the amount of reagents and samples is very small.
[0031] The technical solutions in the embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the pres...
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Abstract
The invention discloses a micro-fluidic chip and application of the micro-fluidic chip in pesticide detection. The micro-fluidic chip comprises a base material and at least one detection unit distributed on the base material. The base material can rotate around a central point. Each detection unit comprises an extraction pool, a micro-channel and a detection pool, wherein the two ends of the micro-channel are communicated with the extraction pool and the detection pool respectively. The extraction pool, the detection pool and the central point are located on the same line. The extraction pool and the detection pool are located at the near end and the far end of the central point respectively. By means of micro-fluidic control, online separation and parallel detection of multiple samples can be achieved, wastage of reagents and samples is small, and the detection limit of pesticide is greatly reduced.
Description
technical field [0001] The application belongs to the fields of environmental detection and food safety, and in particular relates to a microfluidic chip and its application in pesticide detection. Background technique [0002] Due to its strong chemical stability, pesticides are easy to remain in crops and soil or other environments. When their concentration reaches a certain value (residual amount), they will accumulate in the body after being eaten by people, causing chronic poisoning. At present, the detection of pesticide residue pollution in my country is divided into three types, organophosphorus pesticides, pyrethroids and fungicides. Organophosphorus pesticides have the characteristics of high efficiency, safety, economy, convenience, and wide application range in the control of agricultural diseases and insect pests. It is the most widely used pesticide in my country at present. Of the 10 chemical pesticides listed in the "blacklist" of China's environmental priori...
Claims
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IPC IPC(8): B01L3/00G01N21/64
CPCB01L3/5027B01L3/502753B01L2200/10B01L2300/0848G01N21/643G01N2021/6432G01N2021/6482
Inventor 刘静静聂富强顾志鹏亓琳琳刘珊珊
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD