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Red alga gracilariopsis lemaneiformis polysaccharide and preparation thereof, anti-tumor activity detection method and application

A technology of asparagus polysaccharides and asparagus, which is applied in the field of biomedicine, can solve problems such as large side effects, and achieve good anti-tumor effects, significant anti-tumor effects, and enhanced anti-tumor effects

Active Publication Date: 2016-05-04
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common tumor drugs, radiotherapy, chemotherapy and other treatment methods are effective but have relatively large side effects

Method used

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  • Red alga gracilariopsis lemaneiformis polysaccharide and preparation thereof, anti-tumor activity detection method and application
  • Red alga gracilariopsis lemaneiformis polysaccharide and preparation thereof, anti-tumor activity detection method and application
  • Red alga gracilariopsis lemaneiformis polysaccharide and preparation thereof, anti-tumor activity detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Extraction and purification of embodiment 1 asparagus polysaccharide

[0037] Extraction: Asparagus was collected from Wenzhou, Zhejiang Province in October 2014. It was washed repeatedly to remove sediment and other debris, dried and crushed in the shade, and stored in fresh-keeping bags. Weigh 100g asparagus dry powder, add 700mLddH 2 O, neutralized with NaOH to adjust the pH to 10. Incubate in an 80° C. water bath for 4 hours with intermittent stirring. The above solution was centrifuged at 8000rpm / min for 15min, and the supernatant was taken. In order to reduce the loss of polysaccharides, add 5mLddH2O to the precipitate, adjust the pH to 10 with NaOH, keep it in a water bath at 80°C for 4 hours, stir intermittently during this period, then centrifuge the solution at 8000rpm / min for 15min, and take the supernatant again . The supernatant obtained by the first and second centrifugation was combined to improve the polysaccharide extraction rate, and the pH was adj...

Embodiment 2

[0039] Example 2 Inhibitory effect of asparagus polysaccharides on the growth and proliferation of gastric adenocarcinoma cell MKN45

[0040] Gastric adenocarcinoma cell MKN45 is an adherent cell, and the cells were resuscitated with 5×10 6 Seed in fresh 1640 culture medium (containing 89% RPMI1640 medium, 10% fetal bovine serum FBS, 1% penicillin-streptomycin of 1U / mL) at an inoculation density of / L, and place at 37°C, Saturated humidity, 5% CO 2 cultured in an incubator. The medium was changed every 2 days and subcultured once. Gastric adenocarcinoma MKN45 in the logarithmic growth phase was digested with 0.25% trypsin and counted, and the cells were diluted with RPMI-1640 culture medium containing 10% fetal bovine serum to make 1×10 4 / mL cell suspension, inoculated in three 96-well culture plates, 200 μL per well, so that the cell density in each well was 2×10 3 Each group was set up with 4 parallel wells, and a blank group without cells was set up, cultured for 24 ho...

Embodiment 3

[0044] Example 3 Inhibitory effect of asparagus polysaccharide on the growth and proliferation of lung cancer cell A549

[0045] The lung cancer cell culture method, asparagus polysaccharide administration method, cell proliferation ability detection method, cell viability calculation formula and data analysis method are the same as in Example 2. The result is as follows:

[0046] From image 3 It can be seen that when Asparagus polysaccharide acts on lung cancer A549 cells for 24 hours, as the concentration increases, the growth inhibition and proliferation effects also increase, but within 40 μg / mL, the effect is not obvious (P>0.05). When treated for 48 hours, the growth and proliferation abilities of lung cancer cells were 49.813%, 47.677%, 50.042% and 48.949% when the concentrations were 50, 60, 80 and 100 μg / mL (P50 ) were 50 and 45 μg / mL, respectively.

[0047] It can be seen that compared with the inhibitory effect of the control group on the inhibition of proliferat...

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Abstract

The invention discloses marine traditional Chinese medicine gracilariopsis lemaneiformis polysaccharide derived from red alga plant gracilariopsis lemaneiformis, discloses a synergistic effect of the gracilariopsis lemaneiformis polysaccharide serving as anti-tumor drugs, especially a synergist of cis-platinum for the first time, and further discloses an application of the gracilariopsis lemaneiformis polysaccharide to preparation of drugs or healthcare products or food for treating tumors. The gracilariopsis lemaneiformis polysaccharide can serve as a synergist of the anti-tumor drugs. The anti-tumor effect is achieved mainly by retraining growth and propagation of tumor cells, and the anti-tumor effect is achieved for gastric adenocarcinoma, lung cancer and carcinoma of the uterine cervix. The invention further discloses a preparation method of the gracilariopsis lemaneiformis polysaccharide and a detection method of the anti-tumor effect of the gracilariopsis lemaneiformis polysaccharide. A new way is provided for further utilization of gracilariopsis lemaneiformis serving as marine drug resources, and a new choice is provided for development of the anti-tumor drugs for treating or preventing tumors through the application of the gracilariopsis lemaneiformis polysaccharide to preparation of the anti-tumor drugs or anti-cancer healthcare products or food.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a polysaccharide derived from large seaweed, a preparation method thereof, a detection method of anti-tumor activity and an application thereof, in particular to the extraction and purification of a red alga Asparagus polysaccharide, its preparation method, and its anti-tumor activity. A method for detecting tumor activity and its use in the preparation of antitumor drugs or as synergists. Background technique [0002] With the aging of the population, the destruction of the ecological environment, the emergence of unhealthy lifestyles and food safety issues, the incidence and mortality of cancer continue to rise, which has brought serious harm to people's lives, health and society. Common tumor drugs, radiotherapy, chemotherapy and other treatment methods have good effects but relatively large side effects. Traditional Chinese medicine alone or as an adjunct to Western medicine in the ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K36/04A61P35/00
CPCA61K36/04A61K2236/331A61K2236/51A61K2236/53C08B37/00
Inventor 康亚妮赵小东邵志峰
Owner SHANGHAI JIAO TONG UNIV
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