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Oak acorn tannin extraction and starch thick mash fermentation coupling method

A technology of thick mash fermentation and acorn powder, which is applied in the direction of fermentation, etc., can solve the problems of long extraction period, small environmental pollution, and difficulty in industrialization, and achieve the effects of reducing cooking energy consumption, reducing waste water discharge, and improving economic benefits

Inactive Publication Date: 2016-05-04
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has the advantages of high product yield and extraction rate, and little environmental pollution; the disadvantages are long extraction cycle, high energy consumption, and difficulty in industrialization
[0006] The development of thick mash fermentation technology of starch-based raw materials is becoming more and more perfect, but there is little research on the fermentation technology of thick mash with acorns as raw materials at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Get dry cork oak acorns, shell them, crush them through a 100-mesh sieve, weigh 50g and place them in a 500mL Erlenmeyer flask, prepare 40% ethanol (v / v) as the extraction agent, according to the mass-volume ratio of 1:20 (g / mL) Mix the acorn powder with the extraction agent, stir magnetically at 700r / min, 60°C, stir and extract for 3h, filter, repeat the extraction 3 times, combine the three extractions, and measure the accumulation of tannins three times by the Folin-Denis method The extraction rate is 81.1%, and the residual acorn tannin is 1.4%. Freeze-dry the remaining solid, weigh 30g and place it in a 250mL Erlenmeyer flask (another equivalent amount of original acorn starch without detannin treatment was taken as a control, and the following operations were performed simultaneously for comparison), according to the mass volume ratio of 1:2.3 (g / mL) add deionized water, adjust the pH to 5.3~6.8, add α-amylase (30U / g acorn powder), liquefy at 90°C for 90min, the...

Embodiment 2

[0031] Get dry cork oak acorns, shell them, and crush them through an 80-mesh sieve, weigh 50g and place them in a 500mL Erlenmeyer flask, prepare 50% ethanol (v / v) as the extraction agent, according to the mass-to-volume ratio of 1:10 (g / mL) Mix the acorn powder with the extraction agent, stir magnetically at 700r / min, 60°C, stir and extract for 8h, filter, repeat the extraction for 6 times, combine the extracts for six times, and measure the tannin three times by Folin-Denis method The cumulative extraction rate is 85.6%, and the residual content of acorn tannin is 1.1%. The remaining solid was freeze-dried, weighed 30g and put it in a 250mL Erlenmeyer flask, added deionized water according to the mass volume ratio (g / mL) of 1:2.3, adjusted the pH to 5.3-6.8, added α-amylase (50U / g acorn powder), liquefy at 90°C for 90 minutes, then cool down to 60°C, add 250U / g acorn powder with glucoamylase, adjust the pH to 4.5-5.0, cool down to 32°C to obtain saccharification liquid, ta...

Embodiment 3

[0033]Get dried cork oak acorns, shell them, and crush them through a 60-mesh sieve, weigh 50g and place them in a 500mL conical flask, prepare 30% ethanol (v / v) as the extraction agent, and use a mass-to-volume ratio of 1:30 (g / mL) Mix the acorn powder with the extraction agent, stir magnetically at 700r / min, 60°C, stir and extract for 6h, filter, repeat the extraction 5 times, combine the extracts five times, and measure the tannin three times by the Folin-Denis method The cumulative extraction rate is 82.3%, and the residual content of acorn tannin is 1.31%. The remaining solid was freeze-dried, weighed 30g and put it in a 250mL Erlenmeyer flask, added deionized water according to the mass volume ratio (g / mL) of 1:2.8, adjusted the pH to 5.3-6.8, added α-amylase (30U / g acorn powder), liquefy at 90°C for 90min, then cool down to 60°C, add compound saccharification agent (glucoamylase 250U / g acorn powder, α-amylase 20U / g acorn powder, xylanase 60U / g acorn powder, Acid prote...

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Abstract

The invention relates to an oak acorn tannin extraction and starch thick mash fermentation coupling method. According to the method, technical measures such as ''an organic solvent extraction method'', ''a simultaneous saccharification and fermentation method'', ''a thick mash fermentation method'' and the like are adopted; the acorn tannin is extracted with a compound ethanol water solution, and by virtue of a novel extracting agent, the linkage between tannin and protein is effectively damaged, the extraction amount of the acorn tannin is increased, and the extraction time is shortened; acorn powder is added with a certain quantity of the extracting agent and is extracted for several times within a certain extraction time, and then, the content of tannin in the acorn is decreased below 1.5%; in a thick mash fermentation system, the acorn powder in which tannin is removed is taken as a raw material, is firstly added with liquifying enzyme under the condition of high solid content and is subjected to high-temperature steaming, and the gelatinization process and the liquifying process are combined, so that the fermentation viscosity is effectively decreased, and the effects of slowly releasing glucose and reducing the glucose effect are achieved; finally, by virtue of synchronous or stepwise diastatic fermentation, the steaming energy consumption is reduced, the time is shortened, the draining of wastewater is reduced, and the benefit is increased.

Description

technical field [0001] The invention belongs to the field of tannin extraction technology and starch thick mash fermentation, and specifically relates to using Quercus acorns as raw materials, obtaining tannin through an organic solvent extraction method and simultaneously preparing acorn starch, and applying the acorn starch to thick mash In the fermentation system, the comprehensive utilization of acorn raw materials is realized. Background technique [0002] The consumption of fossil fuels is increasing day by day, and biofuels are increasingly recognized and favored by people as a new type of renewable clean energy, while fermentation raw materials have become the main factor limiting the development of biofuels. [0003] Acorn is the fruit of oak plant species, known as "wood grain" and "wood corn" in ancient times. Acorns are widely distributed in various regions of my country, and there are various types (there are more than 60 species of Quercus). They can grow in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12P19/14C12P19/04
CPCC12P7/42C12P19/04C12P19/14
Inventor 张栩刘瑞亮谭天伟
Owner BEIJING UNIV OF CHEM TECH
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