Preparation method of enzymolytic rice protein with telomerase protection function

Abstract

The invention relates to a preparation method of enzymolytic rice protein with telomerase protection function, which is characterized by comprising the following steps: 1) pretreating rice by pulverization and filtration, adding water, and stirring uniformly; 2) boiling in a pressure cooker at 60-120 DEG C for 30-60 minutes; 3) cooling to 40-60 DEG C, adding Bacillus subtilis accounting for 0.1-10 wt% of the mixture, fermenting at 40-60 DEG C under the humidity of 50-90% for 24-72 hours, and after the fermentation finishes, taking the fermentation filtrate for later use; 4) putting the fermentation filtrate in an ultrasonic tank, carrying out ultrasonic treatment under the frequency of 5-100 KHz, and adding a compound protein enzyme solution to form an enzymolysis solution; 5) regulating the pH value of the enzymolysis solution to 6.0-8.0, and carrying out enzymolysis at 25-50 DEG C for 60-240 minutes; and 6) inactivating the enzyme at the high temperature of 60-80 DEG C to obtain the enzymolytic rice protein with telomerase protection function. The method has the advantages of low proteinase consumption, high rice protein yield and high small-molecular peptide content; and the obtained rice protein has the effect of increasing the telomere length.

Description

Technical field

[0001] The invention relates to the field of biotechnology, in particular to a preparation method of enzymatically hydrolyzed rice protein with telomerase protection. Background technique

[0002] Human normal cells will become senescent cells after dozens of natural passages, and each cell division shortens the chromosomes, leading to the maturation and aging of the cells. In order to inhibit the aging of such cells, it is necessary to protect the telomeres in the chromosomes. Telomere is a special structure at the end of eukaryotic chromosomes. It is essentially a repetitive sequence that maintains the integrity of chromosomes. Telomerase is a ribonucleoprotein body composed of protein and RNA. It is an RNA-dependent DNA polymerase that can add telomere fragments to the ends of telomeres to repair the original length of telomeres. According to scientific research, injecting telomerase into senescent cells can extend the length of telomeres and make cells young...

Examples

Embodiment 1

[0041] A preparation method of hydrolyzed rice protein with telomere protection, adopts the following steps:

[0042] 1) After the rice is pretreated by pulverizing and filtering, add water and stir evenly to prepare a fermentation stock solution with a material-to-liquid ratio of 1:80g / L;

[0043] 2) Put the fermentation stock solution in a pressure cooker to 100 o Cooking at the temperature of C for 30 minutes;

[0044] 3) The temperature of the raw fermentation liquid after cooking is lowered to 60 o After C, insert Bacillus subtilis with 10% raw material ratio, and maintain the fermentation temperature at 60 o C, the fermentation humidity is 80%, the fermentation time is 64h, after the fermentation, the filtrate is filtered and used;

[0045] 4) Put the fermentation filtrate obtained in step 3) into an ultrasonic tank for ultrasonic treatment at a frequency of 100KHZ, and then add modified trypsin: modified papain: PEG-100 stearate with a mass ratio of 1:1:1 Compound protease solu...

Embodiment 2

[0049] A preparation method of hydrolyzed rice protein with telomere protection, adopts the following steps:

[0050] 1) After the rice is pretreated by crushing and filtering, add water and stir evenly to prepare a fermentation stock solution with a material-to-liquid ratio of 1:1g / L;

[0051] 2) Put the fermentation stock solution in a pressure cooker to 60 o Steaming at C temperature for 60min;

[0052] 3) The temperature of the raw fermentation liquid after cooking is lowered to 40 o After C, add Bacillus subtilis with a raw material ratio of 0.1% and maintain the fermentation temperature at 40 o C. The fermentation humidity is 50%, the fermentation time is 24h, and the filtrate is filtered and used after fermentation;

[0053] 4) Put the fermentation filtrate obtained in step 3) in an ultrasonic tank for ultrasonic treatment at a frequency of 5KHZ, and then add modified trypsin: modified papain: PEG-100 stearate with a mass ratio of 1:10:10 Compound protease solution;

[0054] 5) ...

Embodiment 3

[0057] A preparation method of hydrolyzed rice protein with telomere protection, adopts the following steps:

[0058] 1) After the rice is pretreated by pulverizing and filtering, add water and stir evenly to prepare a fermentation stock solution with a material-to-liquid ratio of 1:50g / L;

[0059] 2) Put the fermentation stock solution in a pressure cooker to 75 o Cooking at C temperature for 45min;

[0060] 3) The temperature of the raw fermentation liquid after cooking is cooled to 45 o After C, insert Bacillus subtilis with a raw material ratio of 5% and maintain the fermentation temperature at 45 o C, the fermentation humidity is 70%, the fermentation time is 36h, after the fermentation, the filtrate is filtered and used;

[0061] 4) Put the fermentation filtrate obtained in step 3) into an ultrasonic tank for ultrasonic treatment at a frequency of 50KHZ, and then add modified trypsin: modified papain: PEG-100 stearate with a mass ratio of 1:5:3 Compound protease solution;

[0062...

Technical field

[0001] The invention relates to the field of biotechnology, in particular to a preparation method of enzymatically hydrolyzed rice protein with telomerase protection. Background technique

[0002] Human normal cells will become senescent cells after dozens of natural passages, and each cell division shortens the chromosomes, leading to the maturation and aging of the cells. In order to inhibit the aging of such cells, it is necessary to protect the telomeres in the chromosomes. Telomere is a special structure at the end of eukaryotic chromosomes. It is essentially a repetitive sequence that maintains the integrity of chromosomes. Telomerase is a ribonucleoprotein body composed of protein and RNA. It is an RNA-dependent DNA polymerase that can add telomere fragments to the ends of telomeres to repair the original length of telomeres. According to scientific research, injecting telomerase into senescent cells can extend the length of telomeres and make cells young...

Examples

Embodiment 1

[0041] A preparation method of hydrolyzed rice protein with telomere protection, adopts the following steps:

[0042] 1) After the rice is pretreated by pulverizing and filtering, add water and stir evenly to prepare a fermentation stock solution with a material-to-liquid ratio of 1:80g / L;

[0043] 2) Put the fermentation stock solution in a pressure cooker to 100 o Cooking at the temperature of C for 30 minutes;

[0044] 3) The temperature of the raw fermentation liquid after cooking is lowered to 60 o After C, insert Bacillus subtilis with 10% raw material ratio, and maintain the fermentation temperature at 60 o C, the fermentation humidity is 80%, the fermentation time is 64h, after the fermentation, the filtrate is filtered and used;

[0045] 4) Put the fermentation filtrate obtained in step 3) into an ultrasonic tank for ultrasonic treatment at a frequency of 100KHZ, and then add modified trypsin: modified papain: PEG-100 stearate with a mass ratio of 1:1:1 Compound protease solu...

Embodiment 2

[0049] A preparation method of hydrolyzed rice protein with telomere protection, adopts the following steps:

[0050] 1) After the rice is pretreated by crushing and filtering, add water and stir evenly to prepare a fermentation stock solution with a material-to-liquid ratio of 1:1g / L;

[0051] 2) Put the fermentation stock solution in a pressure cooker to 60 o Steaming at C temperature for 60min;

[0052] 3) The temperature of the raw fermentation liquid after cooking is lowered to 40 o After C, add Bacillus subtilis with a raw material ratio of 0.1% and maintain the fermentation temperature at 40 o C. The fermentation humidity is 50%, the fermentation time is 24h, and the filtrate is filtered and used after fermentation;

[0053] 4) Put the fermentation filtrate obtained in step 3) in an ultrasonic tank for ultrasonic treatment at a frequency of 5KHZ, and then add modified trypsin: modified papain: PEG-100 stearate with a mass ratio of 1:10:10 Compound protease solution;

[0054] 5) ...

Embodiment 3

[0057] A preparation method of hydrolyzed rice protein with telomere protection, adopts the following steps:

[0058] 1) After the rice is pretreated by pulverizing and filtering, add water and stir evenly to prepare a fermentation stock solution with a material-to-liquid ratio of 1:50g / L;

[0059] 2) Put the fermentation stock solution in a pressure cooker to 75 o Cooking at C temperature for 45min;

[0060] 3) The temperature of the raw fermentation liquid after cooking is cooled to 45 o After C, insert Bacillus subtilis with a raw material ratio of 5% and maintain the fermentation temperature at 45 o C, the fermentation humidity is 70%, the fermentation time is 36h, after the fermentation, the filtrate is filtered and used;

[0061] 4) Put the fermentation filtrate obtained in step 3) into an ultrasonic tank for ultrasonic treatment at a frequency of 50KHZ, and then add modified trypsin: modified papain: PEG-100 stearate with a mass ratio of 1:5:3 Compound protease solution;

[0062...

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