A method for making corn mature seed floury endosperm nail polish-assisted complete slicing
A production method and nail polish technology, which are applied in the preparation of test samples and other directions, can solve the problems of unwell starch-rich mature seeds, tedious and time-consuming resin embedding process, and difficulty in obtaining endosperm slices, etc. Promotional, easy-to-observe effects
Active Publication Date: 2018-01-30
YANGZHOU UNIV
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Problems solved by technology
At present, the research on the morphology and structure of corn endosperm mainly focuses on developing seeds, but there are few studies on mature seeds, especially the research on floury endosperm. The main reasons are as follows: paraffin sectioning technique, but this method is not suitable for mature starch-rich seeds
②Using resin to embed mature corn seeds, although slices can be made, but the corn seeds are too large to obtain complete endosperm slices, and the resin embedding process is cumbersome and time-consuming, and the embedding agent is expensive
③ Some people in foreign countries use the dry cutting method to slice corn seeds, but it is only suitable for the horny endosperm, while the powdery endosperm cannot be completed, and the dry cutting method cannot obtain complete seed slices
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[0030] Below in conjunction with example accompanying drawing, the present invention will be further described:
[0031] 1. Preparation of sliced samples:
[0032] Seed pasting and fixing: First, use a file to grind the mature corn grains along the cross-cutting direction to the target section, and then use a sharp blade or wire saw to cross-cut the middle part of the corn seed to obtain a sample piece; use a file on the top of the resin base with a suitable size and uniform shape Grind it flat and wipe it clean with alcohol. After the alcohol dries, add an appropriate amount of super glue, place the sample block horizontally in the glue on the resin base, press lightly for 10 seconds, and leave it overnight to ensure a firm paste. The cross-sectional area of the resin base should be larger than that of the sample to prevent the sample from shaking during slicing. At the same time, the resin base should be smaller than the size of the sample clamp so that the base can be c...
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Abstract
The invention discloses a method for making mature corn seed floury endosperm assisted complete slices with nail polish, which comprises the steps of sticking and fixing seeds, trimming, embedding with nail polish, trimming, semi-thin sectioning, section dyeing and observation, etc. The characteristics are: ① Use strong glue to paste and fix the mature seeds on the resin base; ② Use colorless and transparent nail polish as the embedding agent to quickly embed the endosperm tissue; ③ Establish a quick and easy method for the powdery endosperm of mature corn seeds Dry cutting technique to obtain complete slices of seeds; ④ Slices can be used for various purposes, such as staining with iodine solution or polarized light to observe the shape and size of starch grains and the spatial distribution in endosperm tissue. The invention solves the problem that the complete slices of corn mature seeds cannot be prepared by paraffin slices and resin slices, and at the same time overcomes the defect that the dry cutting method cannot prepare powdery endosperm slices, thereby providing technical support for studying the morphology and structure of the mature corn seed powdery endosperm.
Description
technical field [0001] The invention relates to the technical field of microscopic tissue sectioning of plants, in particular to a method for making complete sectioning of corn mature seed floury endosperm. Background technique [0002] Corn is one of the main source crops for human food, animal feed and industrial raw materials. Corn seeds contain a large endosperm, which is an important nutrient storage tissue for corn, and its development and filling determine the weight and quality of the seeds. Corn endosperm is divided into horny and farina, the former endosperm cells are full, while the latter endosperm cells are not full. Starch is the most important storage material of endosperm, and its shape, size and distribution in endosperm tissue have always been a matter of great concern to maize breeders. [0003] To observe the morphological structure of corn endosperm, the seeds must be sliced first. At present, the research on the morphology and structure of corn end...
Claims
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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 韦存虚赵凌霄潘婷王娟
Owner YANGZHOU UNIV