Gray mold resistance identified seedling stage inoculation method for capsicum

A technology for disease resistance identification and botrytis cinerea, which is applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc. Death and other problems, to achieve the effect of accurate and reliable identification results, good stability of results, and uniform incidence

Inactive Publication Date: 2016-05-11
INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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Problems solved by technology

Botrytis cinerea can cause damage at both the seedling stage and the adult plant stage, while gray mold at the seedling stage often causes the death of the whole plant, or even death in pieces, resulting in more serious consequences
Although the in vitro inoculation method is simple, because the pepper plants are young at th

Method used

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Examples

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Effect test

example 1

[0019] Taking 11 capsicum cultivars as research objects, their resistance to Botrytis cinerea was identified by growing point inoculation method.

[0020] 1. Preparation of inoculum.

[0021] Botrytis cinerea strains ( Botrytis cinerea ) was isolated and purified from Botrytis cinerea diseased seedlings with typical gray mold layer collected from pepper greenhouses in Nanchang, Jiangxi Province. The purification process is as follows: pick a small amount of mold layer (conidia and conidiophores) from the collected diseased seedlings with a sterilized inoculation needle and inoculate them on a potato dextrose agar medium (PDA) plate (petri dish). Cultivate at 20°C for 2 days, pick Botrytis cinerea mycelium from the edge of the colony and insert it on a new PDA medium plate, transfer the mycelia on the edge of the colony again after 2 days, and repeat this until the mycelium grown in the medium does not contain other bacteria. The purified Botrytis cinerea mycelium was inocul...

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Abstract

The invention relates to a gray mold seedling-stage inoculation identification method for capsicum and belongs to the technical field of plant protection. The method comprises the steps of culturing botrytis cinerea, collecting botrytis cinerea spores, preparing a 10<6>/mL botrytis cinerea spore suspension, sowing capsicum seeds in sterilized soil, putting the capsicum seeds into a greenhouse with the temperature of 20-25 DEG C, dropwise adding the spore suspension to seedling center growth points for inoculation after each seedling grows to a four-leaf period, carrying out closed moisture preserving on the greenhouse, and carrying out gray mold investigation 21 days later. The method has the technical characteristics of seedling stage identification, simplicity and convenience in operation, high efficiency, rapidness and accurate and reliable identification results. The method is mainly applied to the fast screening of disease-resistant germplasm from capsicum resource varieties and the disease resistance identification on the varieties and serves the selective breeding of disease-resistant new varieties and the rational layout of disease-resistant varieties.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for inoculating and identifying capsicum botrytis at the seedling stage, especially a method for screening capsicum botrytis-resistant resource materials. Background technique [0002] In recent years, with the adjustment of the rural industrial structure, the cultivation of pepper greenhouses in my country has developed rapidly. Due to the low temperature, high humidity and long duration of condensation in the greenhouse in winter and spring, it is very suitable for the occurrence of Botrytis cinerea. Therefore, the disease has become one of the most important diseases in the production of peppers in greenhouses in my country. If the disease loss is mild, the yield will be reduced by 15% to 20%, and if it is severe, the yield will be reduced by more than 30%. But so far, there are few studies on Botrytis cinerea, and the research on the identification of resis...

Claims

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Application Information

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IPC IPC(8): A01G7/06C12Q1/02
Inventor 黄蓉黄瑞荣胡建坤陈学军方荣缪南生
Owner INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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