Method for biosynthesizing nano-silver through photoinduction of imperata leaf extract

A biosynthesis and extraction technology, applied in nanotechnology and other directions, can solve the problems of carcinogenicity of stabilizers or dispersants, harm to human body or environment, and high requirements for instruments and equipment, achieving fast synthesis speed, good stability, high The effect of antibacterial activity

Inactive Publication Date: 2016-05-11
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods include vacuum evaporation, sputtering and laser ablation, etc. This type of method requires high equipment and expensive production costs.
Chemical methods include microemulsion method, electroplating method, redox method and electrochemical reduction method, etc., but the chemical reagents used are harmful to the human body or the environment to a certain extent, and some stabilizers or dispersants are even carcinogenic.

Method used

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  • Method for biosynthesizing nano-silver through photoinduction of imperata leaf extract
  • Method for biosynthesizing nano-silver through photoinduction of imperata leaf extract
  • Method for biosynthesizing nano-silver through photoinduction of imperata leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Biosynthesis of nano-silver particles by the extract of Imperata imperinae

[0032] a. Preparation of biological matrix

[0033] The picked leaves of Imperata imperata were washed with deionized water, dried in an oven at 60°C, and then dried into powder with a pulverizer, and stored for future use.

[0034] Weigh 1g of Imperata imperata powder, add 20mL of deionized water, shake at 37°C, 200rpm for 10min; centrifuge at 12000rpm for 20min, collect the supernatant, and freeze-dry to obtain the extract of Imperata imperata leaf, which is used as a matrix for nano-silver synthesis.

[0035] b. Synthesis of nano silver

[0036] Add 1 mM AgNO3 solution to the synthetic matrix with a concentration of 10 mg / mL, and react for 120 min under the irradiation of LED lamp (1000 lx).

[0037] c. Characterization of nano silver

[0038] The synthesis of nano-silver can be characterized by color change or its strong absorption in the visible region. The reaction system wit...

Embodiment 2

[0039] Example 2 Biosynthesis of nano-silver particles by the extract of Imperata imperinae

[0040] a. Preparation of biological matrix

[0041] The picked leaves of Imperata imperata were washed with deionized water, dried in an oven at 60°C, and then dried into powder with a pulverizer, and stored for future use.

[0042] Weigh 1g of Imperata imperata powder, add 20mL of deionized water, shake at 37°C, 200rpm for 10min; centrifuge at 12000rpm for 20min, collect the supernatant, and freeze-dry to obtain the extract of Imperata imperata leaf, which is used as a matrix for nano-silver synthesis.

[0043] b. Synthesis of nano silver

[0044] Add 1 mM AgNO3 solution to the synthetic matrix with a concentration of 50 mg / mL, and react for 120 min under LED light irradiation (1000 lx).

[0045] c. Characterization of nano silver

[0046] At a concentration of 50mg / mL of biological matrix, a reddish-brown color is generated, and a strong absorption in the visible region is produc...

Embodiment 3

[0047] Example 3 Biosynthesis of nano-silver particles by the extract of Imperata imperinae

[0048] a. Preparation of biological matrix

[0049] The picked leaves of Imperata imperata were washed with deionized water, dried in an oven at 60°C, and then dried into powder with a pulverizer, and stored for future use.

[0050] Weigh 1g of Imperata imperata powder, add 20mL of deionized water, shake at 37°C, 200rpm for 10min; centrifuge at 12000rpm for 20min, collect the supernatant, and freeze-dry to obtain the extract of Imperata imperata leaf, which is used as a matrix for nano-silver synthesis.

[0051] b. Synthesis of nano silver

[0052] Add 2mM AgNO3 solution to the synthetic matrix with a concentration of 50mg / mL, and react for 120min under sunlight irradiation (50000lx).

[0053] c. Characterization of nano silver

[0054] Such as figure 2 shown without adding AgNO 3 The control sample of the solution has no visible region absorption; add 2mM AgNO 3 In solution, a...

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Abstract

The invention relates to the technical field of biology, in particular to a method for biosynthesizing nano-silver through photoinduction of imperata leaf extract. The method comprises the following steps: 1) picked imperata leaves are cleaned, dried and grinded to powder; ultrapure water is added in the powder for oscillating, extracting and centrifugating to obtain supernatant; and the supernatant is frozen and dried to obtain the imperata leaf extract; and 2) the imperata leaf extract in the step 1) is taken as a reaction matrix; AgNO3 solution is added, so that the concentration of the reaction matrix in obtained mixed solution is 10-50 mg/mL, and the concentration of AgNO3 is 1-3 mM; and nano-silver particles are prepared through reaction under sunlight irradiation or LED lamp irradiation. The biosynthesizing method is safe, environment-friendly, wide in synthetic material source and fast in synthesizing speed, can finish the reaction within 90-120 minutes, and is a green, safe and efficient biosynthesizing method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to a method for biosynthesizing nano-silver from extracts of leaves of Imperata prawns induced by light. Background technique [0002] Nano-silver is an atom or atomic cluster with a particle size of 1-100nm. The quantum mechanical effect on the nanometer scale endows nano-silver with various excellent physical, chemical and optical properties, and is widely used in antibacterial materials, molecular detection and catalysts, etc. Field (see reference 1 for details: ChaloupkaK, MalamY, SeifalianAM. Trends Biotechnol., 2010, 28:580-588). According to different principles, the preparation of nano silver can be divided into three categories: physical method, chemical method and biosynthesis method. Physical methods include vacuum evaporation, sputtering, and laser ablation. These methods have high requirements for equipment and expensive production costs. Chemical m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B82Y40/00
CPCB22F9/24B82Y40/00
Inventor 魏雪团张璟司果果邹俊范瑞梁
Owner HUAZHONG AGRI UNIV
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