Method for biosynthesizing nano-silver through photoinduction of imperata leaf extract
A biosynthesis and extraction technology, applied in nanotechnology and other directions, can solve the problems of carcinogenicity of stabilizers or dispersants, harm to human body or environment, and high requirements for instruments and equipment, achieving fast synthesis speed, good stability, high The effect of antibacterial activity
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Embodiment 1
[0031] Example 1 Biosynthesis of nano-silver particles by the extract of Imperata imperinae
[0032] a. Preparation of biological matrix
[0033] The picked leaves of Imperata imperata were washed with deionized water, dried in an oven at 60°C, and then dried into powder with a pulverizer, and stored for future use.
[0034] Weigh 1g of Imperata imperata powder, add 20mL of deionized water, shake at 37°C, 200rpm for 10min; centrifuge at 12000rpm for 20min, collect the supernatant, and freeze-dry to obtain the extract of Imperata imperata leaf, which is used as a matrix for nano-silver synthesis.
[0035] b. Synthesis of nano silver
[0036] Add 1 mM AgNO3 solution to the synthetic matrix with a concentration of 10 mg / mL, and react for 120 min under the irradiation of LED lamp (1000 lx).
[0037] c. Characterization of nano silver
[0038] The synthesis of nano-silver can be characterized by color change or its strong absorption in the visible region. The reaction system wit...
Embodiment 2
[0039] Example 2 Biosynthesis of nano-silver particles by the extract of Imperata imperinae
[0040] a. Preparation of biological matrix
[0041] The picked leaves of Imperata imperata were washed with deionized water, dried in an oven at 60°C, and then dried into powder with a pulverizer, and stored for future use.
[0042] Weigh 1g of Imperata imperata powder, add 20mL of deionized water, shake at 37°C, 200rpm for 10min; centrifuge at 12000rpm for 20min, collect the supernatant, and freeze-dry to obtain the extract of Imperata imperata leaf, which is used as a matrix for nano-silver synthesis.
[0043] b. Synthesis of nano silver
[0044] Add 1 mM AgNO3 solution to the synthetic matrix with a concentration of 50 mg / mL, and react for 120 min under LED light irradiation (1000 lx).
[0045] c. Characterization of nano silver
[0046] At a concentration of 50mg / mL of biological matrix, a reddish-brown color is generated, and a strong absorption in the visible region is produc...
Embodiment 3
[0047] Example 3 Biosynthesis of nano-silver particles by the extract of Imperata imperinae
[0048] a. Preparation of biological matrix
[0049] The picked leaves of Imperata imperata were washed with deionized water, dried in an oven at 60°C, and then dried into powder with a pulverizer, and stored for future use.
[0050] Weigh 1g of Imperata imperata powder, add 20mL of deionized water, shake at 37°C, 200rpm for 10min; centrifuge at 12000rpm for 20min, collect the supernatant, and freeze-dry to obtain the extract of Imperata imperata leaf, which is used as a matrix for nano-silver synthesis.
[0051] b. Synthesis of nano silver
[0052] Add 2mM AgNO3 solution to the synthetic matrix with a concentration of 50mg / mL, and react for 120min under sunlight irradiation (50000lx).
[0053] c. Characterization of nano silver
[0054] Such as figure 2 shown without adding AgNO 3 The control sample of the solution has no visible region absorption; add 2mM AgNO 3 In solution, a...
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