Method for predicting genetic toxicity with human bone mesenchymal stem cells

Abstract

The invention provides a screening method for predicting potential genetic toxicity of chemicals and environmental samples by means of human bone mesenchymal stem cells, and the method is rapid, has early-stage predictability and is based on biomarkers. The genetic toxicity of test substances is predicted through one or a plurality of genetic markers of ATM, ATR, p53, p21, H2AX, Gadd45a and Bax. Due to the fact that contact exposure is conducted on the chemicals or environmental samples and hBMSCs, and a real-time quantitative polymerase chain reaction method serves as a foundation of detection, and the change degree of expression quantity of the genetic markers is quantitatively detected to judge the potential genetic toxicity of the test substances. According to the screening method for predicting the potential genetic toxicity of the chemicals and the environmental samples by means of the human bone mesenchymal stem cells, the problems that in genetic toxicity evaluation, for animal tests and in vitro tests, the test cycle is long, the end point lag of genetic toxicity detection exists, and species differences exist when test results are extrapolated to human bodies are successfully solved, and an efficient, sensitive and accurate evaluation method is provided for genetic toxicity detection of the chemicals and the environmental samples.

Description

technical field [0001] The invention relates to a method for evaluating early genetic toxicity of chemicals or environmental samples, which can be used in fields involving human health or environmental risk evaluation, such as new drug development, pesticide development, new material development, chemical substance and food safety evaluation, and environmental monitoring. Background technique [0002] In the development process of new drugs, pesticides, chemicals, food, cosmetics and disinfectants in my country, genotoxicity tests are officially included in the safety evaluation criteria or test specifications. Genotoxicity is closely related to carcinogenicity and plays an important role in carcinogenicity test screening. In addition, sensitive, rapid and accurate methods are also required to monitor the presence of genotoxic substances in water sources, air and occupational environments. [0003] Commonly used in vitro tests for genotoxicity detection at this stage mainly...

AI Technical Summary

Problems solved by technology

However, these in vitro methods have many limitations: (1) the rate of false positive results is high; (2) cells are grown in a two-dimensional space in vitro and in logarithmic growth phase, which is similar to the complex three-dimensional environment in vivo (3) The detection systems used in these methods are all microorganisms or animal cell strains, so the data obtained have low correlation with the human body system, so that the experimental data cannot be effectively extrapolated

The genotoxicity in vitro experiments and animal models commonly used in existing chemical safety evaluations cause great uncertainty in the evaluation results, especially when the results are extrapolated to humans, which may cause the chemical to stop continuing research and development due to false positive results, or Its toxic effects are only exposed after a large number of applications, causing potential harm to the environment and human health

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