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Chlorophyll fluorescence detection device and method

A technology of chlorophyll fluorescence and detection devices, which is applied in the field of fluorescence detection, can solve the problems that there is no further explanation, the high intensity of the effect light cannot be guaranteed, and the uniformity of light cannot be guaranteed, so as to achieve high intensity and uniformity, improve efficiency and accuracy Effect

Active Publication Date: 2016-05-11
ZHEJIANG UNIV
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Problems solved by technology

The light source in the chlorophyll fluorescence detection process in this patent document uses a triangular arrangement of three LED light sources. Without further explanation, it cannot guarantee that the uniformity of illumination in the field of view of the camera is fully achieved, and the high intensity of the applied illumination cannot be guaranteed.

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  • Chlorophyll fluorescence detection device and method
  • Chlorophyll fluorescence detection device and method
  • Chlorophyll fluorescence detection device and method

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Embodiment Construction

[0038] Such as figure 1As shown, the chlorophyll fluorescence detection device of the present embodiment includes: an LED lighting source 1 as an excitation light source, a CCD camera 2 as an imaging unit, and a red filter 3 is arranged in front of the CCD camera 2 to accommodate plants, an excitation light source, and an imaging unit A closed box 4, an FPGA module 5 that outputs a control signal to control the duty cycle of the excitation light source, a computer 6 and a power supply 7.

[0039] The FPGA module 5 simultaneously outputs the CCD control signal and the control signal of the measuring light to keep in sync, the FPGA communicates with the computer through the serial port, and finally the computer sends out the control signal. The data collected by the CCD camera 2 is processed by a computer.

[0040] LED lighting source 1 is composed of a plurality of LED lamps. Taking the modulated chlorophyll fluorescence detection process as an example, the arrangement of the ...

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Abstract

The invention discloses a chlorophyll fluorescence detection device. The chlorophyll fluorescence detection device comprises an excitation light source for exciting a plant, a camera shooting unit for acquiring plant images, a control unit, and a light shielding space for containing the plant, the excitation light source and the camera shooting unit. The control unit is used for enabling the excitation light source to emit measuring light and acting light of different light intensities by adjusting the duty ratio of drive circuit control signals of the excitation light source, and when the excitation light source emits the acting light, controls signal for measuring light pulses are inserted into intervals of the control signals. The control signals for image acquisition of the camera shooting unit are kept consistent with the control signals for the measuring light pulses. The invention further discloses a chlorophyll fluorescence detection method. By means of the device and method, the plant to be detected is detected and excited through the same light source, real-time and dynamic display of chlorophyll fluorescence images is achieved, and the efficiency and accuracy of chlorophyll detection are improved.

Description

technical field [0001] The invention relates to the technical field of fluorescence detection, in particular to a chlorophyll fluorescence detection device and a detection method. Background technique [0002] Photosynthesis is one of the most important chemical reactions on the earth. It uses sunlight to split water to release most of the oxygen needed on the earth, and at the same time fixes carbon dioxide in the atmosphere to provide glucose for metabolism. In the light reaction process of photosynthesis, the absorbed light energy is transferred. In the process of converting light energy into electrical energy, part of the light energy loss is released in the form of longer wavelength fluorescence. Chlorophyll fluorescence has a very close relationship with photosynthesis. Many changes in photosynthesis process can be reflected by chlorophyll fluorescence. Chlorophyll fluorescence measurement has the characteristics of fast, reliable, sensitive, and no damage to samples....

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/64
Inventor 李海峰王恒卞殷旭徐良刘旭
Owner ZHEJIANG UNIV
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