Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting B and E type human adenovirus nucleic acid and detection method thereof

A human adenovirus and kit technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as high requirements and inability to carry out portable equipment for epidemic prevention and detection, and achieve portable equipment, rapid response, and easy operation. Effect

Active Publication Date: 2016-05-18
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
View PDF4 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, PCR technology has high requirements on instruments and laboratories. These limitations make its application mostly limited to laboratories with good conditions, and cannot be used as portable instruments for on-site epidemic prevention detection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting B and E type human adenovirus nucleic acid and detection method thereof
  • Kit for detecting B and E type human adenovirus nucleic acid and detection method thereof
  • Kit for detecting B and E type human adenovirus nucleic acid and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Experimental Materials:

[0026] RPA lyophilized reagent, buffer and magnesium salt solution were purchased from TwistAmpTM kit of TwistDX Company; lateral flow test strip and its buffer were purchased from HybriDetectMGHD1 kit of Milenia Biotec Company.

[0027] Experimental principle:

[0028] The target nucleic acid is amplified by using biotin-labeled primers and FAM-labeled probes, so that the final amplification product carries both FAM and biotin labels. The front end of the LFD test strip is coated with gold nanoparticles with FAM antibody, and the detection line is coated with biotin antibody. When the reaction solution enters the test strip, the amplification product with FAM and biotin will pass through the antigen antibody. The binding effect forms a complex of biotin antibody-nucleic acid-nano-gold particles on the detection line and develops the color. There is also a quality control line on the lateral flow test strip, which is coated with immobilized a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting B and E type human adenovirus nucleic acid and a detection method thereof. The kit for detecting the B and E type human adenovirus nucleic acid comprises RPA freeze-dried particles, an RPA reaction primer, an RPA reaction probe, an RPA buffer solution, a magnesium salt solution, a transverse flow test strip, a buffer solution, double distilled water and an adenovirus genome DNA. The kit for detecting the B and E type human adenovirus nucleic acid has the advantages that the transverse flow test strip is utilized for carrying out visual qualitative detection on an RPA nucleic acid amplification product, the whole reaction is carried out at the constant temperature of 38 DEG C, a stripe on the test strip can be observed by virtue of naked eyes within 5-20 minutes, results are read and discriminated, and a special detecting instrument is not needed during detection. The kit disclosed by the invention has the characteristics of high sensitivity, strong specificity, easiness in operation, quick reaction and portability of instruments and is especially applicable to bedside rapid diagnosis of adenovirus, epidemic prevention and disease control.

Description

technical field [0001] The invention relates to a virus nucleic acid detection kit, in particular to a kit for detecting type B and E human adenovirus nucleic acids and a detection method thereof. Background technique [0002] Human adenovirus is a kind of non-enveloped double-stranded DNA virus, which is divided into 7 serological groups A-G. More than 60 serotypes have been found. HAdV infection can cause acute adenoviral pneumonia, acute gastroenteritis, Keratitis, celiac disease and acute interstitial nephritis and other diseases. As a common opportunistic pathogen, adenovirus exists in the population for a long time, and can cause outbreaks in densely populated populations, such as military and school personnel. [0003] Different subspecies of adenovirus can cause different diseases. Subspecies C mainly cause upper respiratory tract infection in children, subspecies F of human adenovirus can cause infectious diarrhea, and subspecies B and E can cause acute febrile res...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/6804C12Q1/6844C12Q2521/507C12Q2521/101C12Q2565/625
Inventor 徐东刚邢微微孙魁付文亮
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com