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Integral kit detecting glycosylated hemoglobin and detection method thereof

A technology of glycosylated hemoglobin and kit, which is applied to the integrated kit for detecting glycosylated hemoglobin and its detection field, which can solve the problems of being unsuitable for communities and grass-roots hospitals, high cost of instruments, and low accuracy, so as to improve the screening speed , good specificity and simple structure

Inactive Publication Date: 2016-05-25
山东省内分泌与代谢病研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] For grassroots medical units, the above methods are either expensive for reagents and instruments, or complicated to operate, or have low accuracy and poor stability, and are not suitable for communities and grassroots hospitals.

Method used

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  • Integral kit detecting glycosylated hemoglobin and detection method thereof
  • Integral kit detecting glycosylated hemoglobin and detection method thereof
  • Integral kit detecting glycosylated hemoglobin and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Please refer to the attached figure 1 , with figure 1 It is a structural schematic diagram of an integrated kit for detecting glycosylated hemoglobin according to an embodiment of the present invention.

[0048] Such as figure 1 As shown, the embodiment of the present invention provides an integrated kit for detecting glycosylated hemoglobin, including: a reaction analysis pad 1, an affinity reaction solution microwell 2 on the right side of the reaction analysis pad 1 containing an affinity reaction solution , the cleaning liquid microwell 3 on the right side of the affinity reaction liquid microwell 2 and the barcode area 4 on the right side of the cleaning liquid microwell 3 .

[0049] In an integrated kit for detecting glycosylated hemoglobin in this embodiment, the micropore 2 of the affinity reaction solution has a pore diameter of 5 mm and a height of 8 mm, and contains 250 μL of the affinity reaction solution. The affinity reaction solution includes the follow...

Embodiment 2

[0066] Such as figure 1 As shown, the embodiment of the present invention provides an integrated kit for detecting glycosylated hemoglobin, including: a reaction analysis pad 1, an affinity reaction solution microwell 2 on the right side of the reaction analysis pad 1 containing an affinity reaction solution , the cleaning liquid microwell 3 on the right side of the affinity reaction liquid microwell 2 and the barcode area 4 on the right side of the cleaning liquid microwell 3 .

[0067] In an integrated kit for detecting glycosylated hemoglobin in this embodiment, the microwell 2 of the affinity reaction solution has a pore diameter of 6 mm and a height of 10 mm, and contains 250 μL of the affinity reaction solution. The affinity reaction solution includes the following components: Minute:

[0068] Glycine 150mmol / L,

[0069] Zinc chloride 20mmol / L,

[0070] Magnesium chloride 20mmol / L,

[0071] Potassium chloride 500mmol / L,

[0072] Sodium azide 1.0g / L,

[0073] Adjust...

Embodiment 3

[0086] Such as figure 1 As shown, the embodiment of the present invention provides an integrated kit for detecting glycosylated hemoglobin, including: a reaction analysis pad 1, an affinity reaction solution microwell 2 on the right side of the reaction analysis pad 1 containing an affinity reaction solution , the cleaning liquid microwell 3 on the right side of the affinity reaction liquid microwell 2 and the barcode area 4 on the right side of the cleaning liquid microwell 3 .

[0087] In an integrated kit for detecting glycosylated hemoglobin in this embodiment, the micropore 2 of the affinity reaction solution has a pore diameter of 5 mm and a height of 8 mm, and contains 250 μL of the affinity reaction solution. The affinity reaction solution includes the following components: Minute:

[0088] Glycine 100mmol / L,

[0089] Zinc chloride 15mmol / L,

[0090] Magnesium chloride 35mmol / L,

[0091] Potassium chloride 150mmol / L,

[0092] Sodium azide 0.35g / L,

[0093] Adjust...

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Abstract

The invention provides an integral kit detecting glycosylated hemoglobin and a detection method thereof. The kit comprises a reaction analysis pad, an affinity reaction liquid micropore positioned in the right side of the reaction analysis pad and filled with affinity reaction liquid, a cleaning fluid micropore positioned in the right side of the affinity reaction liquid micropore and filled with cleaning fluid and a code area positioned in the right side of the cleaning fluid micropore. The integral kit detecting the glycosylated hemoglobin only needs a trace of whole blood sample during detection to quantitatively detect the content of the glycosylated hemoglobin in 3-5min, thereby improving the screening speed, and has the advantages of high sensitivity, good specificity, and simple structure, and is simple in preparing method and easy for large scale production. The detection method thereof only needs a trace of whole blood sample during the detection to quantitatively detect the content of the glycosylated hemoglobin in 3-5min, and has high sensitivity and good specificity.

Description

technical field [0001] The invention relates to the technical field of glycosylated hemoglobin detection, in particular to an integrated kit for detecting glycosylated hemoglobin and a detection method thereof. Background technique [0002] Diabetes mellitus is a group of endocrine and metabolic diseases whose etiology and pathogenesis are not yet fully understood, and its incidence rate is second only to cardiovascular diseases and tumors. At present, the incidence of diabetes is on the rise, and it is a worldwide public health problem that seriously threatens human health. Traditional diabetes diagnosis and treatment monitoring use fasting blood glucose, postprandial blood glucose, and oral glucose tolerance test, etc., but blood glucose parameters only represent the instantaneous blood glucose level when blood is drawn, and glycosylated hemoglobin (GHb) is used as the gold standard to reflect long-term blood glucose levels , is also an important indicator for monitoring ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N30/00
CPCG01N1/28G01N30/00
Inventor 黄亚丽魏芳赵柯丁文宇
Owner 山东省内分泌与代谢病研究所