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Cell line selection method for tea plant containing different catechin content and components

A catechin and cell line technology, applied in the field of plant cell line screening, can solve the problems of complicated operation, inability to achieve rapid detection, and long time required for the detection of catechin content and components.

Inactive Publication Date: 2009-08-05
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the detection of catechin content and components in tea tree callus or cell lines takes a long time, the operation is complicated, and the problem of rapid detection cannot be achieved, the present invention provides a tea tree containing Screening method of cell lines with different catechin contents and components

Method used

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  • Cell line selection method for tea plant containing different catechin content and components
  • Cell line selection method for tea plant containing different catechin content and components
  • Cell line selection method for tea plant containing different catechin content and components

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Experimental program
Comparison scheme
Effect test

Embodiment

[0073] Major equipment

[0074] 1. Scalpel; 2. High-pressure steam sterilizer; 3.2.5*10 silica gel GF-254 plate; 4.9cm petri dish; 5. Centrifuge; 6. pH meter; 7. Ultra-clean bench; 8. High-efficiency liquid Phase Chromatography (HPLC)

[0075] Materials and Reagents

[0076] 1. Materials:

[0077] Young stems of Camellia sinensis (L.) O. Kuntze. var. sinensis cultivar Yun Nan Da YeZhong.

[0078] 2. Main reagents:

[0079] (1) 70% ethanol: by volume absolute ethanol: water=750:282 preparation,

[0080] (2) 0.1% mercuric chloride (HgCl 2 ): take 0.1g mercuric chloride (HgCl 2 ) was dissolved in 100ml pure water,

[0081] (3) Solid medium: in 1LB 5 0.5 mg of 2,4-dichlorophenoxyacetic acid, 0.1 mg of kinetin, 8 g of agar and 30 g of sucrose were added to the medium; the pH was 5.5,

[0082] (4) The liquid medium is: in 1LB 5 0.5 mg of 2,4-dichlorophenoxyacetic acid, 0.1 mg of kinetin and 30 g of sucrose were added to the medium; the pH was 5.5,

[0083] (5) 1% vanillin...

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Abstract

The invention relates to a screening method for the tea plant clone containing different catechuic acid contents and components, which solves the problems that the detection for the catechuic acid contents and the components in the callus or clone of tea plants needs long time and the operation is complex in the screening technology. The screening method includes four operating steps of inducing and successively transferring the clone, screening the callus containing different catechuic acid contents, screening the clone containing different catechuic acid contents, and screening the clone containing different catechuic acid components. The invention can screen the clone containing different catechuic acid contents and components and display the difference of the catechuic acid contents of the callus within the short time of 5-10 min, thereby having the great advantages that the catechuic acid detection can be performed during the successive transfer operation process; a plurality of samples can be analyzed once by the thin layer chromatography and identification of the catechuic acid components of the clone of the tea plant, in addition, a new component can be also found by the thin layer chromatography and identification, thereby the screening of the clone containing different catechuic acid contents and components is accelerated.

Description

technical field [0001] The invention relates to a screening method for plant cell lines, and a screening method for cell lines containing different catechin contents and components in tea trees. Background technique [0002] The synthesis of plant flavonoids is affected by external environmental factors. Factors such as light, temperature, and nutrition will all affect the synthesis of phenolic substances in tea trees. Since there are many limiting factors in studying the biosynthesis and metabolism of phenolic substances in intact tea plants, tea callus and cell suspension culture system have undoubtedly become a good platform for studying the secondary metabolism of tea plants. Screening tea cell lines with different catechin content and components is the prerequisite for the establishment of this good platform. [0003] There are many common screening methods for cell lines containing special substances, and finding an effective and easy special substance detection metho...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02G01N21/78C12N5/04
Inventor 刘亚军高丽萍夏涛
Owner ANHUI AGRICULTURAL UNIVERSITY
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