Fermented milk product with blood pressure reducing function and preparation method thereof
A technology for fermenting dairy products and lowering blood pressure, applied in dairy products, milk preparations, food ingredients, etc., can solve the problem of less high value-added products, improve nutrition and health care efficacy, strong operability and practicability, and improve blood pressure. effect of blood pressure
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[0044] 1) Preparation of reaction solution
[0045] Take 20 μL of inhibitor solution, add 120 μL of 5 mmol / L HHL solution, mix well, place in a water bath at 37°C to preheat for 5 minutes; at the same time, take 20 μL of LACE reagent and place in a water bath at 37°C to preheat for 5 minutes ; After preheating, mix the above mixed solution and ACE reagent thoroughly and evenly as a sample group. The sample group was incubated at a temperature of 37° C. for 60 min, and then 200 μL of 1 mol / L HCl was added to terminate the reaction to obtain a reaction solution. After the reaction solution was filtered through a 0.45 μm filter membrane, it was injected and analyzed by high performance liquid chromatography (HPLC).
[0046] Furthermore, 20 μL of the borate buffer solution was used instead of the inhibitor solution as the first blank control group; 20 μL of the sodium borate buffer solution was used instead of the ACE reagent as the second blank control group.
[0047] 2) Chroma...
Embodiment 1
[0055] A blood pressure-lowering fermented milk product, which is mainly fermented by adding lactic acid bacteria strains to the following raw materials in parts by weight: 85 g of fresh buffalo milk, 5 g of sucrose, 5 g of buffalo milk blood pressure lowering peptide base material, and 0.15 g of taurine; The amount is 0.022836u.
[0056] The preparation method of the buffalo milk hypotensive peptide base material is:
[0057] (1) Standardization of raw milk: using fresh buffalo milk as a raw material, defatting it, adjusting the protein content in the fresh buffalo milk to 4%, to obtain standardized buffalo milk;
[0058] (2) Sterilization: heat the standardized buffalo milk obtained in step (1) to 60° C., and sterilize for 10 minutes;
[0059] (3) Adjusting the pH value: cooling the buffalo milk obtained after step (2) to 55° C., and adjusting the pH value to 10.5;
[0060] (4) Protease hydrolysis: adding protease to the buffalo milk treated in step (3), and hydrolyzing it...
Embodiment 2
[0069] A blood pressure-lowering fermented milk product, which is mainly fermented by adding lactic acid bacteria strains to the following raw materials in parts by weight: fresh buffalo milk 88g, sucrose 6g, buffalo milk blood pressure lowering peptide base material 8g, taurine 0.25g; lactic acid bacteria strains added The amount is 0.0407u.
[0070] The preparation method of the buffalo milk hypotensive peptide base material is:
[0071] (1) Standardization of raw milk: using fresh buffalo milk as a raw material, defatting it, adjusting the protein content in the fresh buffalo milk to 4%, to obtain standardized buffalo milk;
[0072] (2) Sterilization: heat the standardized buffalo milk obtained in step (1) to 65° C., and sterilize for 8 minutes;
[0073] (3) Adjusting the pH value: cooling the buffalo milk obtained after step (2) to 60° C., and adjusting the pH value to 10.0;
[0074] (4) Protease hydrolysis: adding protease to the buffalo milk treated in step (3), and hy...
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