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A desert oligotrophic bacterium dob153 and its application in sand fixation

An oligotrophic, desert-based technology, applied to the application of new oligotrophic bacteria in biological crusts to fix quicksand, in the field of microbial applications, can solve the problems of rare reports of oligotrophic microorganisms and the application of oligotrophic bacteria, and achieve growth rates Fast, strong growth and reproductive ability, and stable genetic characteristics

Inactive Publication Date: 2018-10-26
XINJIANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of literature search showed that, from an environmental point of view, the current oligotrophic bacteria are mainly researched on the water environment, but there are not many reports on oligotrophic microorganisms resistant to barren environments in dry desert areas, and there are no new discoveries similar to oligotrophic bacteria. A report on the application of vegetative bacteria in the fixation of quicksand in biological crusts

Method used

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  • A desert oligotrophic bacterium dob153 and its application in sand fixation
  • A desert oligotrophic bacterium dob153 and its application in sand fixation
  • A desert oligotrophic bacterium dob153 and its application in sand fixation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation, screening and identification of oligotrophic bacteria Bacillus sp.DOB153CGMCC NO.11926

[0021] 1. Isolation and screening of strains

[0022] The desert oligotrophic bacteria used in the present invention are sampled and isolated from the Gurbantunggut Desert in Junggar Basin, Xinjiang, and the strains capable of growing on LB medium are preliminarily screened out according to the analysis of the action mechanism of the bacteria. The microorganisms in the Gurbantunggut Desert in the Junggar Basin of Xinjiang were isolated by the traditional plate culture method, the strains were screened by primary screening and secondary screening, and the strains were purified by plate streaking method, and the bacterial strains were compared on the LB medium. According to the growth and reproduction situation, a batch of well-growing microbial strains were screened out, and a desert oligotrophic bacterial strain numbered DOB 153 with good comprehensive performa...

Embodiment 2

[0036] Example 2: Molecular identification of oligotrophic bacteria Bacillus sp.DOB153CGMCC NO.11926

[0037] 1. PCR amplified DNA sequence of oligotrophic bacteria DOB153 and its sequencing

[0038] Pick a small amount of single colony of oligotrophic bacteria DOB153 strain, put it into an EP tube filled with 25 μL of sterile water, boil it at 100°C for 8-10 minutes, then quickly put it into the ice-water mixture for 5 minutes, centrifuge at 10000r / min, 5min, 4 Store at ℃, take the supernatant when used.

[0039] 16S rDNA gene sequence determination and phylogenetic tree construction: Extract the total DNA of oligotrophic bacteria DOB153 strain according to conventional methods, and use deionized water to dilute the universal primers for PCR amplification of 16S rDNA segments.

[0040] The 50 μl reaction system contained: 5 μl of 10×PCR buffer, 20 pmol of each primer, 1 μl of template DNA, 0.5 U of TaqTM (TaKaRa Company), and 8 μl of dNTP. PCR amplification conditions: pre-...

Embodiment 3

[0045] Example 3: Research on the characteristics of oligotrophic bacteria Bacillus sp.DOB153CGMCC NO.11926

[0046] 1. Experimental process

[0047] The oligotrophic bacterium DOB153 was repeatedly streaked and activated on LB medium, and then used for the preparation of liquid bacterial agent. After inoculation, it was cultured in a shake flask at 37°C for 60 hours.

[0048] (1) Sand fixation test

[0049] Use quicksand to pack 2 disks with a length of 30cm × width 20cm × height 5cm, put the quicksand into the tray, and spray the oligotrophic bacteria DOB153 on the quicksand surface of one of the sand trays, and the other sand tray. An equal amount of tap water served as a control.

[0050] (2) Water retention test

[0051] Take 20 crucibles with known drying constant weight (wherein 10 are sprayed with water for comparison, and 10 are sprayed with bacterium solution of oligotrophic bacteria DOB153) and weighed, denoted as W 1 , respectively weighing 30g of sand into the...

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Abstract

The invention discloses a desert oligotrophic bacterium DOB153 and its application in sand fixation. A desert oligotrophic bacterium Bacillus sp.DOB153 was obtained through isolation, screening, breeding, and domestication in the Gurbantunggut Desert in the Junggar Basin, Xinjiang. The preservation number of the strain is CGMCC NO.11925. The isolated The bacterial solution prepared by the oligotrophic bacteria DOB153 strain is used on the desert surface not only to play a role in sand fixation, but also to provide better water conditions for the germination of some plant seeds with a slower germination rate, so that some plants that cannot germinate due to insufficient soil moisture Get growth and development, not only play a role in sticking sand particles, preventing quicksand activation, but also effectively increasing the number of main microorganisms in the soil, increasing the content of main nutrients in the soil, enhancing the activity of some soil enzymes, and can obviously restore algae crusts. It has a wide range of applications in sand fixation. Value.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to the technical field of the application of a new oligotrophic bacterium in biological crust fixing quicksand. Background technique [0002] Sandy desertification is a serious environmental problem faced by mankind today, and my country is one of the countries most seriously affected by desertification in the world. Environmental deterioration and economic poverty caused by desertification have seriously restricted ecological restoration and economic development, and governance is imminent. my country has carried out desertification research and prevention for half a century, but the desertification land has been expanding and spreading. Scientific research and prevention and control practices show that interdisciplinary and comprehensive integration is the only way to prevent and control desertification. At present, the main method of artificial sand fixation is to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/07
CPCC09K17/14C12N1/205C12R2001/07
Inventor 毛培宏吕杰张伶钱卫东
Owner XINJIANG UNIVERSITY
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