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Application of maff in the diagnosis and differentiation of coronary heart disease and coronary heart disease with diabetes mellitus

A technology for coronary heart disease and diabetes, which is applied in the application field of MAFF in diagnosing and distinguishing coronary heart disease and coronary heart disease with diabetes. Good application prospects, good repeatability

Active Publication Date: 2019-04-19
宫蕊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although great progress has been made in the research of coronary heart disease, there are few studies on the pathogenesis of coronary heart disease combined with diabetes, and the pathogenesis of the two remains to be elucidated

Method used

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  • Application of maff in the diagnosis and differentiation of coronary heart disease and coronary heart disease with diabetes mellitus
  • Application of maff in the diagnosis and differentiation of coronary heart disease and coronary heart disease with diabetes mellitus
  • Application of maff in the diagnosis and differentiation of coronary heart disease and coronary heart disease with diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 sample collection and extraction

[0035] The case group (10 people in total, 2 patients with simple coronary heart disease, and 6 patients with coronary heart disease combined with diabetes mellitus, see Table 1 for clinical information, and the control group (10 people in total) required fasting for at least 12 hours, and the next morning at 7:00- 8:00 At room temperature, draw 10ml of venous blood into an ethylenediaminetetraacetic acid (EDTA) anticoagulant tube, extract peripheral blood mononuclear cell PBMCs, add 1ml Trizol reagent (Invitrogen), mix well, and store the specimen at -80°C. For RNA extraction. All blood samples and pathological results should be authentic and reliable, the research was approved by the ethics committee, and the patients gave informed consent.

[0036] Table 1 Clinical information of patients in the case group

[0037]

[0038] Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) of patients and healthy ...

Embodiment 2

[0039] Example 2 High-throughput sequencing and analysis

[0040] Construction of mRNA library: The 3' end of eukaryotic mRNA has a polyA tail structure. Magnetic beads with Oligo (dT) are used to enrich mRNA. After high-temperature fragmentation, cDNA is synthesized using it as a template. After magnetic bead purification, end repair, base A addition at the 3' end, and sequencing adapters, PCR amplification was performed to construct an mRNA library. According to the results of RNA sample detection, mRNA libraries were constructed for 2 patients with pure coronary heart disease (GX), 6 patients with coronary heart disease and diabetes mellitus (GX_TN) and the normal control group.

[0041] Sequencing: mRNA was sequenced using llumina Hiseq2500 / Miseq second-generation high-throughput sequencing technology, and data processing was completed by removing joints, removing low-quality, and decontaminating processes to obtain final data.

[0042] Expression data analysis: Through t...

Embodiment 3

[0043] Example 3 Expression of MAFF gene in peripheral blood of coronary heart disease, coronary heart disease patients with diabetes mellitus and healthy people

[0044] 1. Materials and methods

[0045] 1. Materials

[0046] The peripheral blood of 65 patients with coronary heart disease, 52 patients with coronary heart disease and diabetes mellitus and 44 healthy people were collected, grouped and numbered.

[0047] 2. Method

[0048] 2.1 Extraction of total RNA from peripheral blood

[0049] use Reagent was used to extract RNA from samples, and the experimental operation was performed according to the product instructions.

[0050] Judgment criteria for RNA quality: the OD260 / OD280 value of the RNA sample is between 1.8 and 2.2; the electrophoretic pattern of total RNA has clear 28S and 18S bands; difference.

[0051] 2.2 Synthesis of cDNA by reverse transcription

[0052] use III Reverse Transcriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse tr...

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Abstract

The invention relates to application of MAFF to diagnosis and distinguishment of a coronary artery disease and a coronary artery disease combined with diabetes. Based on a high-throughput sequencing method, peripheral blood of patients suffering from the coronary artery disease and the coronary artery disease combined with diabetes and healthy people is sequenced; three sets of data, i.e. contrast of the coronary artery disease and health, contrast of coronary artery disease combined with diabetes and health, and the coronary artery disease and the coronary artery disease combined with diabetes; and a differential expression gene MAFF is selected, so that by an experiment, correlation betwen the MAFF gene and the coronary artery disease as well as the coronary artery disease combined with diabetes is verified. The MAFF gene provided by the invention not only can be used as a diagnosis target of the coronary artery disease and the coronary artery disease combined with diabetes, but also can be used for distinguishing the coronary artery disease from the coronary artery disease combined with diabetes, and has very high clinical application value.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of MAFF in diagnosing and distinguishing coronary heart disease and coronary heart disease combined with diabetes. Background technique [0002] Coronary heart disease (CHD) plays an important role in cardiovascular system diseases. It is a heart disease caused by myocardial ischemia, hypoxia or necrosis caused by narrowing or occlusion of the coronary artery caused by atherosclerosis. It is considered to be one of the most common causes of death in the world. In recent years, the incidence of this disease in China has been increasing, and it has become a huge threat to people's health. Diabetes is one of the main risk factors for coronary heart disease. The incidence of coronary heart disease is higher in people with diabetes. The stenosis of the lumen is severe. It has been reported that half of newly diagnosed diabetic patients have large blood vessel damage, sugge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2800/042G01N2800/324
Inventor 宫蕊
Owner 宫蕊
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