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Preparation method of glycyrrhetinic acid monoglucuronide

A technology of glycyrrhetinic acid aldehyde and licorice aldehyde, applied in the field of preparation of glycyrrhetinic acid monoglucuronate, can solve the problems of complex operation, difficult process control, high cost, etc., and achieve scientific and reasonable process, simple and easy operation, The effect of high production efficiency

Active Publication Date: 2016-06-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the product prepared by this method has high purity, it is difficult to realize industrial production
Chinese patent (publication number CN102367463A) discloses a method for producing monoglucuronoglycyrrhetinic acid by indirect fed-batch fermentation, which can effectively improve the yield of GAMG fermentation, but the period of inducing enzyme production in the early stage is longer
Although this method significantly improves substrate utilization and product yield, the operation is complicated and the process is not easy to control
[0006] In summary, the chemical method converts glucuronic acid from glycyrrhizic acid by hydrolysis through the action of acid and alkali, thereby generating glycyrrhetinic acid and glycyrrhetinic acid monoglucuronoglycyrrhetinic acid, but this method is more serious to environmental pollution, and strong acid Alkali will also have a certain destructive effect on the structure of glycyrrhizic acid, resulting in a decrease in the yield of the target product
Biotransformation method, due to complex preparation conditions and process technology, high cost and long cycle, it is difficult to realize industrialization

Method used

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  • Preparation method of glycyrrhetinic acid monoglucuronide

Examples

Experimental program
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Effect test

Embodiment 1

[0036] 1. Preparation of glycyrrhetinic acid monoglucuronic acid extract: first soak licorice with 5% acetic acid solution, the liquid-solid ratio is 3:1, the soaking temperature is 60°C, and the soaking time is 4h; the soaked licorice is drained Dry water, carry out steam explosion treatment, the pressure is 10MPa, and the explosion time is 10min; after the steam explosion pretreatment, the licorice is subjected to microwave-assisted extraction, the extraction liquid-solid ratio is 30:1, the extraction time is 30min, the temperature is 60°C, microwave The power is 300W, the stirring speed is 150rpm, and the monoglucuronic acid glycyrrhetinic acid extract is obtained after the extraction is completed.

[0037] 2. Clarification treatment: Centrifuge the obtained extract of glycyrrhetinic acid monoglucuronide at a speed of 4200 rpm / min for 15 minutes to obtain a clarified solution of glycyrrhetinic acid monoglucuronide, and discard the precipitate.

[0038] 3. Separation and pur...

Embodiment 2

[0041] 1. Preparation of glycyrrhetinic acid monoglucuronic acid extract: first soak licorice with 5% sulfuric acid solution, the liquid-solid ratio is 3:1, the soaking temperature is 60°C, and the soaking time is 4h; the soaked licorice is drained Dry water, carry out steam explosion treatment, the pressure is 10MPa, and the explosion time is 10min; after the steam explosion pretreatment, the licorice is subjected to microwave-assisted extraction, the extraction liquid-solid ratio is 30:1, the extraction time is 30min, the temperature is 60°C, microwave The power is 300W, the stirring speed is 150rpm, and the monoglucuronic acid glycyrrhetinic acid extract is obtained after the extraction is completed.

[0042] 2. Clarification treatment: Centrifuge the obtained extract of glycyrrhetinic acid monoglucuronide at a speed of 4200 rpm / min for 15 minutes to obtain a clarified solution of glycyrrhetinic acid monoglucuronide, and discard the precipitate.

[0043] 3. Separation and pur...

Embodiment 3

[0046] 1. Preparation of glycyrrhetinic acid monoglucuronic acid extract: first soak licorice with 5% sulfuric acid, the liquid-solid ratio is 2:1, the soaking temperature is 70°C, and the soaking time is 3h; drain the soaked licorice Moisture, steam explosion treatment, the pressure is 10MPa, and the explosion time is 15min; after the steam explosion pretreatment, the licorice is subjected to microwave-assisted extraction, the extraction liquid-solid ratio is 50:1, the extraction time is 60min, the temperature is 70°C, and the microwave power The stirring speed is 600W, the stirring speed is 250rpm, and the extract of monoglucuronic acid glycyrrhetinic acid is obtained after the extraction is completed.

[0047] 2. Clarification treatment: Centrifuge the obtained extract of glycyrrhetinic acid monoglucuronide at a speed of 3600 rpm / min for 15 minutes to obtain a clarified solution of glycyrrhetinic acid monoglucuronide, and discard the precipitate.

[0048] 3. Separation and ...

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Abstract

Belonging to the technical field of biochemical engineering, the invention discloses a preparation method of glycyrrhetinic acid monoglucuronide. The preparation method includes: taking licorice as the raw material, firstly preparing an extracted solution of glycyrrhetinic acid monoglucuronide, then performing clarification treatment, further conducting separation and purification, and finally drying the purified glycyrrhetinic acid monoglucuronide so as to obtain glycyrrhetinic acid monoglucuronide. The preparation method performs pretreatment on licorice through an external physical field, the process is scientific and reasonable, green and environmental protection, the operation is simple and practicable, and can realize continuous production. The glycyrrhetinic acid monoglucuronide prepared by the method has the characteristics of low cost, high production efficiency, high purity, easy popularization and high added value, can be used as a food additive and drug precursor, and provides raw material for the food and pharmaceutical industries.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a preparation method of glycyrrhetinic acid monoglucuronate. Background technique [0002] Glycyrrhizauralensis, also known as sweet grass root, red licorice, old country, sweet grass, sweet root, etc., is one of the traditional Chinese herbal medicines commonly used in my country. Licorice is flat and sweet in nature, has the functions of invigorating the spleen and replenishing qi, clearing heat and detoxification, eliminating phlegm and relieving cough, relieving spasms and relieving pain, and reconciling various medicines. It has extremely wide clinical application value. [0003] Glycyrrhizic acid (GL) is the main active substance in licorice, which has certain pharmacological activities, such as protecting the liver, anti-virus and anti-cancer. Glycyrrhizic acid has high sweetness, its sweetness is 170-200 times that of sucrose, and it is used as a...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 高彦祥樊蕊
Owner CHINA AGRI UNIV
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