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A kind of water-soluble rare earth doped nanocrystal and its preparation method and application

A rare earth doping, nanocrystal technology, applied in the fields of nanotechnology, nano-optics, nanotechnology, etc., can solve the problems of low fluorescence quantum yield, poor fluorescence stability, complicated preparation process, etc., and achieve high fluorescence quantum yield and fluorescence. High stability and improved detection efficiency

Active Publication Date: 2019-06-18
广纳达康(广州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional fluorescent probes based on rare earth complexes have complicated preparation process, poor fluorescence stability, and low fluorescence quantum yield, which limit their wider application to a certain extent.
At the same time, in view of the complexity of biological systems and the requirements of clinical testing, it is often necessary to analyze multiple components simultaneously, and the existing detection probes based on time-resolved fluorescence technology obviously cannot meet this requirement.

Method used

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  • A kind of water-soluble rare earth doped nanocrystal and its preparation method and application
  • A kind of water-soluble rare earth doped nanocrystal and its preparation method and application
  • A kind of water-soluble rare earth doped nanocrystal and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 La 0.2 f 3 :Tb 0.2 , Ce 0.6 Water-soluble Rare Earth Doped Nanocrystals

[0032] 1) Weigh 1.6mmol LaCl 3 ·7H 2 O, 1.6mmol TbCl 3 ·6H 2 O and 4.8 mmol CeCl 3 ·7H 2 O, dissolved in 100mL ultrapure water, stirred to make it completely dissolved, and mixed solution A was obtained;

[0033] 2) Weigh 24mmol NH 4 F, dissolved in 40mL of ultrapure water to obtain NH 4 F aqueous solution B; said ultrapure water refers to water with a resistivity ≥ 15 megohm cm at 25°C;

[0034] 3) Mix NH under stirring condition 4 F aqueous solution B was added dropwise to the mixed solution A at a constant speed, and the peristaltic pump controlled the dropping rate to 1.5mL / min. After the dropwise addition, the stirring reaction was continued at room temperature for 1h, and the reaction solution was centrifuged at 10000rpm for 30min to obtain precipitate C;

[0035] 4) Precipitate C was dispersed by adding 30mL ultrapure water for 30min and then transferred to a 50mL hyd...

Embodiment 2

[0037] Example 2 La 0.8 f 3 :Tb 0.2Water-soluble Rare Earth Doped Nanocrystals

[0038] 1) Weigh 6.4mmol LaCl 3 ·7H 2 O and 1.6 mmol TbCl 3 ·6H 2 O, dissolved in 100mL ultrapure water, stirred to make it completely dissolved, and mixed solution A was obtained;

[0039] 2) Weigh 21mmol NH 4 F, dissolved in 40mL of ultrapure water to obtain NH 4 F water solution B;

[0040] 3) Mix NH under stirring condition 4 F aqueous solution B was added dropwise to the mixed solution A at a constant speed, and the peristaltic pump controlled the dropping rate to 2mL / min. After the dropwise addition, the stirring reaction was continued at room temperature for 1h, and the reaction solution was centrifuged at 12000rpm for 30min to obtain precipitate C;

[0041] 4) Precipitate C was dispersed by adding 30mL ultrapure water and ultrasonic for 30min, then transferred to a 50mL hydrothermal kettle, reacted at 200°C for 12h, cooled to room temperature, and obtained La 0.8 f 3 :Tb 0.2 Wa...

Embodiment 3

[0042] Example 3 La 0.6 f 3 :Tb 0.2 , Ce 0.2 Water-soluble Rare Earth Doped Nanocrystals

[0043] 1) Weigh 4.8mmol LaCl 3 ·7H 2 O, 1.6mmol TbCl 3 ·6H 2 O and 1.6 mmol CeCl 3 ·7H 2 O, dissolved in 100mL ultrapure water, stirred to make it completely dissolved, and mixed solution A was obtained;

[0044] 2) Weigh 20mmol NH 4 F, dissolved in 40mL of ultrapure water to obtain NH 4 F water solution B;

[0045] 3) Mix NH under stirring condition 4 The aqueous solution B of F was added dropwise to the mixed solution A at a constant speed, and the dropping rate was controlled by a peristaltic pump to be 1.2mL / min. After the dropwise addition, the stirring reaction was continued at room temperature for 1 hour, and the reaction solution was centrifuged at 14000rpm for 30 minutes to obtain a precipitate C;

[0046] 4) Precipitate C was dispersed by adding 30mL ultrapure water for 30min and then transferred to a 50mL hydrothermal kettle, reacted at 180°C for 24h, cooled to ro...

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Abstract

The invention relates to water-soluble rare-earth-doped nanocrystals and a preparation method and application thereof. The preparation method comprises the steps of dissolving lanthanide compounds into ultra-pure water, dropwise adding an NH4 F aqueous solution at the constant speed under the stirring condition, and preparing and obtaining the water-soluble rare-earth-doped nanocrystals through a hydro-thermal method. By changing the mixing ratio of the lanthanide compounds, the water-soluble rare-earth-doped nanocrystals different in fluorescence lifetime are obtained. According to the water-soluble rare-earth-doped nanocrystals and the preparation method and application thereof, the water-soluble rare-earth-doped nanocrystals are prepared directly in the aqueous solution, raw materials are easy to obtain, the method is simple, the condition is mild, and controllability is great; the mean grain diameter of the obtained water-soluble rare-earth-doped nanocrystals ranges from 40 nm to 50 nm, the fluorescence lifetime is adjustable, the fluorescence quantum yield is high, and fluorescence stability is high; the prepared water-soluble rare-earth-doped nanocrystals can be widely applied to 'monochromatic and multi-marker' analysis of cells, viruses, tissue pathological sections and the like through the time resolution fluorescence imaging technology.

Description

technical field [0001] The invention relates to a water-soluble rare earth-doped nano crystal, a preparation method and application thereof, and belongs to the technical field of chemiluminescent materials. Background technique [0002] With the development of bioanalytical science and bioengineering technology, how to realize the rapid and highly sensitive detection of components in complex biological systems has become more and more important. However, the background interference from biological tissues often seriously affects the detection sensitivity and the accuracy of the results in the actual process. The time-resolved fluorescence analysis technology based on rare earth fluorescent complexes with long fluorescence lifetimes has been applied in the fields of clinical detection and biochemical analysis because it can effectively eliminate the background interference of the system to be measured. However, the traditional fluorescent probes based on rare earth complexes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/85B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/7772G01N21/6428
Inventor 杨祥良胡军唐永安
Owner 广纳达康(广州)生物科技有限公司
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