Composition for suppressing neuraminidase activity comprising geranylated flavonoid derived from paulownia tomentosa as active ingredient
A technology of neuraminidase and active ingredients, which is applied in the field of compositions that inhibit neuraminidase activity, and can solve problems such as side effects, low efficacy, and low bioavailability
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Embodiment 1
[0360] Preparation of Paulowniatomentosa Extract
[0361]To prepare Paulownia tomentosa extract, 1 kg of Paulownia tomentosa fruit (Jinju, Republic of Korea) dried in the shade was crushed and washed with methanol The extraction was repeated three times at room temperature. The Paulownia tomentosa methanol extract obtained by the above process was concentrated under reduced pressure to obtain 118 g of a dark brown crude extract (yield: 11.8%).
Embodiment 2
[0362] Preparation of Paulowniatomentosa Fraction
[0363] After dissolving 118g of the dark brown crude extract of Paulownia tomentosa in the in distilled water and ethyl acetate mixed solvent, the ethyl acetate was classified, passed through anhydrous Na 2 SO 4 The ethyl acetate fractionated above was dried to obtain 16 g of fractions.
Embodiment 3
[0364] Isolation and Purification of Novel Compounds from Paulowniatomentosa Extract
[0365] Isolation and purification of geranyl flavonoid (geranylatedflavonoid) compound
[0366] In order to separate and purify flavones from the ethyl acetate fraction obtained by the method of , silica gel column chromatography (200-400mesh) was carried out using a mixed solvent of hexane and ethyl acetate (50:11:1), Seven fractions (A-E) were obtained.
[0367] Fraction A (4.2 g) was subjected to silica gel column chromatography using a mixed solvent of hexane and acetone (100:11:1) to obtain nine fractions (A1-A9). Using Sephadex (LH-20, PharmaciaBiotech AB, Uppsla, Sweden) and by 80% methanol solvent, fractions A3 and A4 (460 mg) mixed with No. 5 and No. 9 compounds were experimented, and No. 5 compounds were isolated ( 25mg) and No. 9 compound (18mg). Fraction B (8.9 g) was subjected to silica gel column chromatography using a mixed solvent of chloroform and acetone (100:11:1) to o...
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