Compound microbial agent for low-temperature fermentation of straws

A compound microbial inoculant and low-temperature fermentation technology, which is applied in the fields of microorganism-based methods, microorganisms, and biological organic parts treatment, etc. problem, to achieve the effect of fast decay

Active Publication Date: 2016-06-22
北京中农国泰科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bacterial agent is composed of Pseudomonas fluorescens, Bacillus cereus, Bacillus subtilis and Bacillus circulans. Although this bacterial agent can decompose straw and produce ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A compound microbial agent for straw low-temperature fermentation, including Trichoderma viride 6×10 6 cfu / g, Aspergillus niger 4.0×10 5 cfu / g, Bacillus subtilis 2.0×10 9 cfu / g, Lactococcus 2.0×10 6 cfu / g, Pediococcus lactis 1.5×10 6 cfu / g, Bacillus circulans 1.0×10 8 cfu / g. Take 10 g of the above-mentioned microbial inoculum and dissolve it in 1 L of water.

[0020] Choose 150kg of corn stalks and crush them to a length of 1-3cm. The crushed stalks are wetted with water according to the weight ratio of 1:1 to water, mixed well while adding water, and then stacked for 1-2 days to facilitate the stalks to fully absorb water.

[0021] Inoculate the diluted composite bacterial agent into crushed and water-absorbing corn stalks, adjust the water content to 60%-70% (W / W), fill and compact it in an airtight plastic bag at an ambient temperature of about 10°C Fermented for 12 days, dried at room temperature and crushed to obtain the finished product of corn stalk decomp...

Embodiment 2

[0023] A compound microbial inoculant for straw low-temperature fermentation, the compound microbial inoculum includes Trichoderma viride 3 × 10 7 cfu / g, Aspergillus niger 2.0×10 5 cfu / g, Bacillus subtilis 1.0×10 9 cfu / g, Lactococcus 2.0×10 6 cfu / g, Pediococcus lactis 0.5×10 6 cfu / g, Bacillus circulans 6.0×10 7 cfu / g.

[0024] Select 150kg of wheat straw and crush it to a length of 1-3cm. The crushed stalks are wetted with water according to the weight ratio of 1:1 to water, mixed well while adding water, and then stacked for 1-2 days to facilitate the stalks to fully absorb water.

[0025] Inoculate the diluted composite bacterial agent into crushed and water-absorbing wheat straw, adjust the water content to 60%-70% (W / W), fill it up and compact it in an airtight plastic bag at an ambient temperature of about 8°C Fermented for 12 days, dried at room temperature and crushed to obtain the finished wheat straw decomposing agent.

Embodiment 3

[0027] A compound microbial inoculum for straw low-temperature fermentation, the compound microbial inoculum includes Trichoderma viride 6 × 10 8 cfu / g, Aspergillus niger 2.5×10 5 cfu / g, Bacillus subtilis 1.0×10 10 cfu / g, Lactococcus 1.5×10 6 cfu / g, Pediococcus lactis 7×10 5 cfu / g, Bacillus circulans 5.0×10 7 cfu / g.

[0028] Select 150kg of soybean straw and crush it to a length of 1-3cm. The crushed stalks are wetted with water according to the weight ratio of 1:1 to water, mixed well while adding water, and then stacked for 1-2 days to facilitate the stalks to fully absorb water.

[0029] Inoculate the diluted composite bacterial agent into crushed and water-absorbing soybean stalks, adjust the water content to 60%-70% (W / W), fill it up and compact it in an airtight plastic bag at an ambient temperature of about 12°C Fermented for 11 days, dried at room temperature and crushed to obtain the finished product of soybean straw decomposing agent.

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PUM

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Abstract

The invention discloses a compound microbial agent for low-temperature fermentation of straws. The compound microbial agent comprises trichoderma viride, aspergillus niger, bacillus subtilis, lactococcus, pediococcus acidilactici and bacillus circulans. In order to prolonging normal-temperature storage time of the compound microbial agent, the compound microbial agent further comprises auxiliary materials including a bacterium preserving agent, a carrier, an antioxidant, deionized water, a water retaining agent and the like. By application of the compound microbial agent for low-temperature fermentation of straws, the straws can be decomposed and blackened about 12 days under the low-temperature condition of 4-15 DEG C, the straws are basically thoroughly decomposed at a high speed; due to addition of the auxiliary materials including the bacterium preserving agent, the carrier, the antioxidant, the water retaining agent and the like into the compound microbial agent for low-temperature fermentation of the straws, the compound microbial agent can be stored for a long time at the normal temperature.

Description

technical field [0001] The invention relates to a microbial inoculum, in particular to a composite microbial inoculum for straw low-temperature fermentation. Background technique [0002] Our country is very rich in straw resources. Except for a small part used for livestock feed, a large amount of straw remains unused, and a large amount of remaining straw is burned in the open on the spot. On the one hand, it causes a great waste of resources and serious environmental pollution. On the one hand, it will reduce the content of soil organic matter, resulting in a decrease in soil half and water storage capacity. Therefore, solving the remaining straw problem in my country is extremely important for resource utilization and environmental pollution control. In addition, in many areas of China in the cold season, the biological activity of fermenting microorganisms is low, and the raw materials cannot be fully decomposed and utilized. Therefore, improving straw fermentation in ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/00C05F17/00C12R1/885C12R1/685C12R1/125C12R1/01C12R1/09
CPCC05F11/00C05F17/00C12N1/14C12N1/20C05F11/08Y02W30/40
Inventor 王庆华张梅周艳玲张卉王丽萍张冰万雪冰
Owner 北京中农国泰科技有限公司
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