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Symmetry gene CmCYC2c of chrysanthemum morifolium and application of symmetry gene CmCYC2c

A symmetrical, chrysanthemum technology, applied in applications, genetic engineering, plant genetic improvement, etc., can solve the problems of lack, difficulty in achieving directional cultivation, long cycle, etc., and achieve the effect of combining accurate and reliable

Active Publication Date: 2016-06-22
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

At present, the functional research of chrysanthemum CYC2 gene has not been reported, and the technology of improving chrysanthemum flower shape by using it is even more lacking.
In addition, the main method for cultivating new varieties of chrysanthemums is still traditional hybrid breeding. Compared with molecular breeding, it not only has a long cycle and is difficult to achieve directional cultivation

Method used

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  • Symmetry gene CmCYC2c of chrysanthemum morifolium and application of symmetry gene CmCYC2c
  • Symmetry gene CmCYC2c of chrysanthemum morifolium and application of symmetry gene CmCYC2c
  • Symmetry gene CmCYC2c of chrysanthemum morifolium and application of symmetry gene CmCYC2c

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Embodiment 1

[0032] 1. Cloning of the CmCYC2c gene of ground cover chrysanthemum 'Guoqing Xiaoliu'

[0033] The extraction material of total RNA was young flower buds of the ground cover chrysanthemum variety 'Guoqing Xiaoliu', which was ground by liquid nitrogen and followed the operating instructions of the General Plant Total RNA Extraction Kit (spin column type, RP3302) of Beijing Biotech Company. . The quality of total RNA was detected by NanoDrop-1000 UV spectrophotometer, and only when A260 / 280 was between 1.8-2.1 and A260 / 230 was greater than 2.0 was used for subsequent experiments. 3'RACE and 5'RACE cDNA templates were performed according to the Clontech SMARTerTM RACE cDNA amplification kit. The 3'RACEGSP forward primer CYC2-3'GSPF1 (SEQ ID NO. 3), the 3'RACE cDNA synthesized in the previous step is used as a template for PCR amplification. 50μL reaction system: 3'RACEcDNA2.5μL, 10xUPM (0.4μM) 5.0μL, 3'GSP (10μM) 1.0μL, 10xAdvantage2PCRBuffer5.0μL, 50xAdvantage2PolymeraseMix1.0μ...

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Abstract

The invention relates to plant genes and particularly discloses a novel symmetry gene CmCYC2c of chrysanthemum morifolium and a novel application of the symmetry gene CmCYC2c in the aspect of regulation of ligulate flower petal traits of the chrysanthemum morifolium, especially, in the aspects of increase of the number of ligulate flower petals and increase of petal length. The nucleotide sequence of the symmetry gene CmCYC2c is represented as SEQ ID NO.1. Based on the gene and the application thereof, the CmCYC2c gene is integrated to a chamomile genome with a transgenic technology, the flower type is primarily and directionally improved, so that the number of the ligulate flowers is increased, the petal length is increased, and a novel and practical method is provided for primary selection of new species of chrysanthemum morifolium varieties with the genetic engineering technology.

Description

technical field [0001] The invention relates to plant genes, in particular to the chrysanthemum symmetry gene CmCYC2c and its application in regulating the petal traits of chrysanthemum ray flowers. Background technique [0002] The flowers of angiosperms have two forms: radial symmetry and left-right symmetry, and bilaterally symmetrical flowers are evolved from radially symmetric flowers. The evolution of bilaterally symmetrical flowers not only enriched the morphology of flowers, but also enhanced the viability of species by improving pollination efficiency (Endress, 1998). Chrysanthemum (Chrysanthemummorifolium) is inflorescence head, each inflorescence is composed of many small flowers, among which the tongue-shaped flowers are female flowers, which are bilaterally symmetrical; the central tubular flowers are hermaphrodite flowers, which are radially symmetrical. The differences in the composition and distribution of ligulate flowers and tubular flowers of chrysanthemu...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/84C12N1/21A01H5/00
CPCC07K14/415C12N15/8205C12N15/827
Inventor 张启翔黄笛刘华孙明潘会堂程堂仁王佳杨炜茹
Owner BEIJING FORESTRY UNIVERSITY
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