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Preparation method of N4-acetyl-sulfamethoxazole residue standard substance in pelteobagrus fulvidraco tissue

A technology of sulfamethoxazole and reference materials, which is applied in the field of aquatic product safety testing, can solve the problems of complex metabolic process of sulfamethoxazole and no known preparation method, etc.

Inactive Publication Date: 2016-06-22
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

but by directly adding N 4 -Acetyl-sulfamethoxazole and sulfamethoxazole are metabolized in aquatic animals to produce N 4 - The way of acetyl-sulfamethoxazole is different. The metabolic process of sulfamethoxazole in aquatic animals is complicated, and it can be combined with aquatic animals in various forms. The detection of added drug residues is different, therefore, it is necessary to prepare a metabolite N of sulfamethoxazole in aquatic animal tissues 4 -Acetyl-sulfamethoxazole residual standard substance for the analysis of sulfamethoxazole metabolites N in aquatic products 4 - the residual amount of acetyl-sulfamethoxazole, the present invention has invented a kind of sulfamethoxazole metabolite N in the peltaceae muscle, liver, kidney, gill and ovarian tissue 4 -Preparation method of acetyl-sulfamethoxazole residual standard substance
[0004] At present, there is no sulfamethoxazole metabolite N in yellow catfish tissue at home and abroad. 4 -A report or patent application on the preparation method of acetyl-sulfamethoxazole residual standard substance

Method used

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  • Preparation method of N4-acetyl-sulfamethoxazole residue standard substance in pelteobagrus fulvidraco tissue
  • Preparation method of N4-acetyl-sulfamethoxazole residue standard substance in pelteobagrus fulvidraco tissue
  • Preparation method of N4-acetyl-sulfamethoxazole residue standard substance in pelteobagrus fulvidraco tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] N in the tissue of a peltatus 4 -The preparation method of acetyl-sulfamethoxazole residual standard substance, its step is:

[0054] A. Select 60g or 80 or 90g of female yellow catfish that has been confirmed to be free of sulfamethoxazole and N4-acetyl-sulfamethoxazole. 3 Temporary culture in the aquarium for one week, used to prepare N 4 - Acetyl-sulfamethoxazole residual standard substance. Freshwater aquaculture conditions, water temperature 25 or 26 ℃, pH 7.9 or 8 or 8.1, continuous aeration, dissolved oxygen value ≥ 5.0mg / L;

[0055] B. Weigh 0.84g sulfamethoxazole and dissolve it in 5mL of dimethylformamide, then dilute with water to 100mL, and prepare 8.4mg / mL sulfamethoxazole solution;

[0056] C, oral administration of sulfamethoxazole solution at a dose of 60 mg / kg body weight of yellow catfish, the yellow catfish after oral administration of sulfamethoxazole is temporarily placed in a 40L aquarium (with 20L water) to observe whether there is any return ...

Embodiment 2

[0068] Sulfamethoxazole in fresh yellow catfish muscles, liver, kidney, gills and ovaries collected by the method in Example 1 after 24h, 72h, 96h, 120h, 144h, 168h, 192h, 216h Oxazole and N 4 - The residual concentration of acetyl-sulfamethoxazole is shown in Table 3.

[0069] Table 3 Sulfamethoxazole and N in muscle, liver, kidney, gill and ovary of yellow catfish at different time 4 - Concentration of acetyl-sulfamethoxazole

[0070]

[0071] Note: ND is not detected

[0072] Other implementation steps are the same as in Example 1.

Embodiment 3

[0074] N in prepared peltatus muscle, liver, kidney, gill and ovary 4 - Acetyl-sulfamethoxazole standard substance homogeneity test

[0075] The muscles, liver, kidneys, gills and ovaries of 5 yellow catfish were collected at 216h after oral administration of sulfamethoxazole using the method in Example 1, and the back muscles of each yellow catfish were collected, peeled and cut into small pieces , using a high-speed homogenizer (IKAULTRA-TURRAXT18basic) to homogenize the muscle of the pelted catfish to prepare N 4 - Acetyl-sulfamethoxazole residual standard substance, stored at -20°C, valid for 3 months. Open the gill cover of the yellow catfish, collect the gill arches and gill filaments of the yellow catfish in a 10mL centrifuge tube, cut them into small pieces with small scissors, and use a high-speed homogenizer (S-10 hand-held high-speed homogenizer) to It is homogenized and stored at -20°C, and the validity period is 3 months; the ovarian tissue of the yellow catfish...

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Abstract

The invention discloses a preparation method of N4-acetyl-sulfamethoxazole residue standard substance in pelteobagrus fulvidraco tissue. The preparation method includes: A, selecting female pelteobagrus fulvidraco which is determined without containing sulfamethoxazole and N4-acetyl-sulfamethoxazole; B, using dimethyl formamide to dissolve sulfamethoxazole, and using water for dilution to obtain a sulfamethoxazole solution; C, orally pouring the sulfamethoxazole solution according to dosage of 60 mg / kg body weight of pelteobagrus fulvidraco, and putting pelteobagrus fulvidraco after going through oral administration in water for observation; D, selecting pelteobagrus fulvidraco without vomiting to prepare N4-acetyl-sulfamethoxazole residue standard substance, collecting back muscle, liver, kidney, gill and ovary of pelteobagrus fulvidraco for homogenization respectively to prepare the N4-acetyl-sulfamethoxazole residue standard substance in the pelteobagrus fulvidraco tissue, freezing and storing the same. The preparation method is easy to implement, simple and convenient to operate and capable of valuing the standard substance prepared and testing uniformity and stability.

Description

technical field [0001] The invention belongs to the technical field of aquatic product safety detection, and more specifically relates to a metabolite N of sulfamethoxazole in the tissue of yellow catfish. 4 -Preparation method of acetyl-sulfamethoxazole residual standard substance, suitable for sulfamethoxazole metabolite N in peltaceae muscle, liver, kidney, gill and ovarian tissue 4 - Preparation of acetyl-sulfamethoxazole residue standard substance. Background technique [0002] Sulfamethoxazole (SMZ), which belongs to broad-spectrum antibacterial drugs, is one of the most sold and used drugs in the world, and is widely used in the prevention and treatment of diseases in livestock, poultry and aquaculture. The human body's long-term intake of animal foods containing sulfonamide drug residues can easily lead to the accumulation of drugs in the body, easily lead to the emergence of bacterial drug resistance, and cause great harm to human health. In recent years, sulfamet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N33/12
CPCG01N1/28G01N33/12
Inventor 刘永涛徐春娟艾晓辉余琳雪宋雅琪杨红
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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