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Nano-selenium mycotoxin antidote and preparation method and application

A technology of mycotoxins and nano selenium, applied in food science and other directions, can solve problems such as body poisoning, achieve mild conditions, realize large-scale production, and strengthen liver detoxification function.

Inactive Publication Date: 2016-06-29
南通海康生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are always a small amount of mycotoxins that are not adsorbed and absorbed by the body, causing toxic effects on the body

Method used

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  • Nano-selenium mycotoxin antidote and preparation method and application
  • Nano-selenium mycotoxin antidote and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The biosynthesis of embodiment 1 red nano selenium

[0026] Draw a certain amount of feed probiotic Bacillus licheniformis. from the storage tube, draw LB agar plate, culture overnight at 37°C, pick a single colony and inoculate into 4mL LB liquid medium, culture overnight at 37°C, 150r / min shaking , inoculated at 1% in 100mL LB liquid medium, cultured with shaking at 37°C and 150r / min for 4h, so that the OD600 value reached 0.4-0.8, added sodium selenite solution with a final concentration of 2mmol / L that was sterilized by filtration, at 37°C, 150r / min shaking continued to cultivate for 24h, Bacillus licheniformis reduced sodium selenite to red elemental selenium ( figure 1 ).

Embodiment 2

[0027] The biosynthesis of embodiment 2 red nano selenium

[0028] Draw a certain amount of feed probiotic Bacillus licheniformis. from the storage tube, draw LB agar plate, culture overnight at 37°C, pick a single colony and inoculate into 4mL LB liquid medium, culture overnight at 37°C, 150r / min shaking , inoculated at 1% in 100mL LB liquid medium, cultured with shaking at 37°C and 150r / min for 4h, so that the OD600 value reached 0.4-0.8, added sodium selenite solution with a final concentration of 1mmol / L that was sterilized by filtration, at 37°C, 100r / min shaking continued to cultivate for 24h, Bacillus licheniformis reduced sodium selenite to red elemental selenium.

Embodiment 3

[0029] The biosynthesis of embodiment 3 red nano selenium

[0030] Draw a certain amount of feed probiotic Bacillus licheniformis. from the storage tube, draw LB agar plate, culture overnight at 37°C, pick a single colony and inoculate into 4mL LB liquid medium, culture overnight at 37°C, 150r / min shaking , inoculated at 1% in 100mL LB liquid medium, cultured with shaking at 37°C and 150r / min for 4h, so that the OD600 value reached 0.4-0.8, added sodium selenite solution with a final concentration of 5mmol / L that was sterilized by filtration, at 37°C, Continue to cultivate for 48 hours with 200r / min shaking, Bacillus licheniformis will reduce sodium selenite to red elemental selenium.

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Abstract

The invention discloses a nano-selenium mycotoxin antidote and a preparation method and application thereof. The nano-selenium mycotoxin antidote is prepared by combining nano-selenium and chitosan. The nano-selenium prepared by means of feed probiotics has the various functions of resisting oxidation, achieving immunity adjustment, resisting mycotoxin and the like, the immunity function of an animal body can be promoted due to the adoption of the functional nano-selenium, the liver detoxification function is enhanced, and clinic symptoms of mycotoxicosis are relieved; the nano-selenium mycotoxin antidote is simple in preparation technology, mild in condition and capable of achieving large-scale production easily.

Description

technical field [0001] The invention relates to a nanometer selenium mycotoxin antidote and its preparation method and application. Background technique [0002] Mycotoxins are a class of toxic secondary metabolites produced during the growth of molds. At present, there are about 300 kinds of mold metabolites that are toxic to humans and animals. Among them, the mycotoxins that have the greatest impact on human and animal health include aflatoxins, Zearalenone, ochratoxin, T-2 toxin, fumonisins and deoxynivalenol, etc. During the production, processing, storage and transportation of crops and feed, due to the pollution of molds and their toxins, feed mildew, lower digestibility, animal mycotoxin poisoning and other hazards have caused huge economic losses to the feed industry and aquaculture industry. What is important is that mycotoxins can not only cause direct poisoning to animals, but also transfer from animals to humans through the food chain, endangering human health....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
Inventor 缪德年屈汶辉廖学文郭佳宏王秀花邹笑天
Owner 南通海康生物科技有限公司
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