Composite cordyceps militaris probiotic enzyme and preparation method thereof

Abstract

The invention discloses a composite cordyceps militaris probiotic enzyme and a preparation method thereof. The method includes the following six steps of preparation of a strain liquid culture medium, preparation of a fermentation strain, preparation of a fermentation substrate, first-time fermentation, second-time fermentation and product obtaining. The preparation method adopts step-by-step two-time fermentation of a cordyceps militaris strain and the composite enzyme, utilizes a cordyceps militaris strain fermentation product as the substrate to perform second-time fermentation of the composite enzyme, native diversity of enzyme components is achieved, the composite cordyceps militaris probiotic enzyme contains the effective components of the cordyceps militaris and effective components of the composite enzyme, and meanwhile D-mannitol produced by the cordyceps militaris is converted into D-mannose which can be absorbed by human. The two times of fermentation are not influenced mutually and can be performed continuously and be continuously performed, the efficiency is high, and the composite cordyceps militaris probiotic enzyme and the preparation method are very suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation production, in particular to a compound probiotic enzyme of Cordyceps militaris and a preparation method thereof. Background technique [0002] As we all know, Cordyceps sinensis is a precious Chinese medicinal material in my country, and it is called "three major Chinese medicine supplements" together with ginseng and velvet antler. "Model species" has become an effective substitute for Cordyceps sinensis and has been widely accepted. On March 18, 2009, the Ministry of Health issued the No. 3 Announcement in 2009, approving Cordyceps militaris as a new resource food, which is a great opportunity for the Cordyceps militaris industry in the field of food processing. The application of Cordyceps militaris has opened the door. The substances that play a role in the efficacy of Cordyceps militaris generally belong to nucleosides, sugar alcohols, polysaccharides, amino acids, enzymes and m...

AI Technical Summary

Problems solved by technology

[0003] At present, the artificial cultivation of Cordyceps militaris mainly includes fruit body cultivation and mycelium fermentation. The fruit body cultivation period is long, the physiological process experienced is complicated, and the taste of the obtained fruit body is poor. In the existing mycelium fermentation, one-time fermentation is generally used. , the product has a single effect and is not easy to absorb, especially the main component D-mannitol in cordycepic acid is difficult to be absorbed by the body, resulting in the loss of nutrients