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Detection method of arecoline

A detection method, the technology of arecoline, which is applied in the field of analytical chemistry, can solve the problems that the detection method of arecoline is rarely reported in literature, and achieve the effect of simple sample pretreatment, easy operation and accurate quantification

Active Publication Date: 2016-07-06
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few literature reports on the detection method of arecoline in betel nut

Method used

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  • Detection method of arecoline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 0.5 g of betel nut sample 1 cut to the size of a grain of rice in a conical flask, add 10 mL of absolute ethanol, ultrasonically extract for 10 min, take 1 mL of the supernatant and analyze it by gas chromatography-mass spectrometry, according to the component peak area Quantification (quantification by external standard method).

[0022] The gas chromatography conditions are as follows:

[0023] The chromatographic column is HP-5MS chromatographic column or equivalent column, the length×inner diameter×film thickness is 30m×0.25mm×0.25μm; the carrier gas is helium; the inlet temperature is 180°C; the constant flow mode, the column flow rate is 1.0mL / min, split ratio 10:1; the heating program is to keep at 50°C for 5min, raise the temperature to 150°C at a rate of 2°C / min, keep for 0min, raise the temperature to 240°C at a rate of 4°C / min, and keep for 10min to obtain The chromatogram is as figure 1 shown.

[0024] The mass spectrometry conditions are as follo...

Embodiment 2

[0028] Weigh 1.5 g of betel nut sample 2 cut to the size of rice grains in a conical flask, add 15 mL of absolute ethanol, ultrasonically extract for 20 min, take 2 mL of the supernatant and analyze it by gas chromatography-mass spectrometry, according to the component peak area Quantification (quantification by external standard method).

[0029] The gas chromatography conditions are as follows:

[0030] The chromatographic column is HP-5MS chromatographic column or equivalent column, the length×inner diameter×film thickness is 30m×0.25mm×0.25μm; the carrier gas is helium; the temperature of the injection port is 210°C; the constant flow mode, the column flow rate is 2.0mL / min, split ratio 20:1; the heating program is to keep at 60°C for 10 minutes, raise the temperature to 170°C at a rate of 5°C / min, hold for 2 minutes, raise the temperature to 260°C at a rate of 6°C / min, and hold for 15 minutes.

[0031] The mass spectrometry conditions are as follows:

[0032] The ioniz...

Embodiment 3

[0035] Weighed 1.0 g of betel nut sample 3, which was cut to the size of a grain of rice, in a conical flask, added 13 mL of absolute ethanol, extracted ultrasonically for 15 min, and took 1.5 mL of the supernatant for analysis by gas chromatography-mass spectrometry. Area quantification (external standard method quantification).

[0036] The gas chromatography conditions are as follows:

[0037] The chromatographic column is HP-5MS chromatographic column or equivalent column, the length×inner diameter×film thickness is 30m×0.25mm×0.25μm; the carrier gas is helium; the temperature of the injection port is 190°C; the constant flow mode, the column flow rate is 1.5mL / min, split ratio 15:1; the heating program is to keep at 55°C for 7 minutes, raise the temperature to 160°C at a rate of 3°C / min, hold for 1 minute, raise the temperature to 250°C at a rate of 5°C / min, and hold for 12 minutes.

[0038] The mass spectrometry conditions are as follows:

[0039] The ionization metho...

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Abstract

The invention relates to a detection method of arecoline in betel nuts, and belongs to the technical field of analytical chemistry.A solvent extraction-gas chromatography-mass spectrometry combined method is adopted to determine the arecoline in the betel nuts.The method comprises the steps that 0.5-1.5 g of shredded betel nuts are weighed and put into a conical flask, 10-15 mL of absolute ethyl alcohol is added, ultrasonic extraction is conducted for 10-20 min, and 1-2 mL of supernatant liquid is taken, analyzed through a gas chromatography-mass spectrometer and quantified through an external standard method.The detection method can be used for detecting the content of the arecoline in the betel nuts, has the advantages that sample pretreatment is easy, operation is easy and convenient, and quantification is accurate and is easy to apply and popularize.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a method for detecting arecoline in betel nuts. Background technique [0002] Arecoline oily liquid, can be mixed with water, ethanol or ether in any proportion. Arecoline has a cholinomimetic effect and has a nicotine-like effect. It is used medically to treat glaucoma and can paralyze tapeworms, so it is also used as an anti-tapeworm medicine, and the effect is better with pumpkin kernels. Can act directly on uterine smooth muscle. In large doses, it can cause the uterus to contract and achieve the purpose of hemostasis. It is clinically used to treat postpartum uterine bleeding, poor uterine involution, and menorrhagia. [0003] At present, there are few literature reports on the detection method of arecoline in betel nut. Therefore, it is very urgent to develop a method for the detection of arecoline in betel nut with simple sample pretreatment, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 张凤梅刘志华司晓喜朱瑞芝申钦鹏刘春波杨光宇何沛王昆淼苏钟璧王晋尤俊衡
Owner CHINA TOBACCO YUNNAN IND
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