A sedum rhizosphere growth-promoting Bacillus arzii and its application
A technology of Bacillus aeruginosa, rhizosphere growth promotion, applied in the application, bacteria, biocide and other directions, to achieve the effect of strong pertinence and growth promotion
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[0019] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0020] 1. Screening process
[0021] Three rhizosphere soil samples of sedum succulents from the production base of sedum succulents in Huanglou Town, Qingzhou City, Shandong Province were collected. Bacterial isolates were isolated from rhizosphere soil samples by serial dilution method. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use.
[0022] to R containing L-tryptophan 2 Bacteria were inoculated in liquid culture medium A, cultured on a shaker at 28° C., 180 rpm, for 4 days. Take 50 microliters of bacterial suspension and drop it on a white ceramic plate, and add 50 microliters of Salkowski colorimetric solution (50ml 35% HCLO 4 +1ml 0.5mol / L FeCl 3 ). The colorim...
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