Microsatellite-labeled primer linked with precocious puberty or no precocious puberty of eriocheir sinensis and application thereof

Abstract

The invention discloses a microsatellite-labeled primer linked with precocious puberty or no precocious puberty of eriocheir sinensis and application thereof. The microsatellite-labeled primer is characterized in that 5 pairs of microsatellite primers are provided, wherein 2 microsatellite sites are linked with the precocious puberty character of the eriocheir sinensis; 3 microsatellite sites are linked with the normal character of the eriocheir sinensis; the primer can be used for identifying whether the eriocheir sinensis has the precocious puberty. The specific identification method comprises the following steps of: taking DNA of a gene group of the eriocheir sinensis to be identified, then carrying out PCR (Polymerase Chain Reaction) augmentation by 5 pairs of microsatellite primers, respectively carrying out polyacrylamide gel electrophoresis on augmentation products, then carrying out silver dyeing staining, and judging the condition of the precocious puberty of the eriocheir sinensis to be identified by the size of strips at different positions. The microsatellite-labeled primer and the application disclosed by the invention have the advantages that normal individuals without precocious puberty can be selected for culture in a targeted manner, so that the normal individuals can be prevented from mixing the young eriocheir sinensis of precocious puberty in the process of early culture, and the development of the eriocheir sinensis in China can be effectively improved.

Description

technical field [0001] The invention relates to a microsatellite marker primer linked with the precocious puberty of Chinese mitten crab and its application, and belongs to the field of animal molecular genetics. Background technique [0002] Chinese mitten crab (Eriocheirsinensis) is an important economic aquatic animal in my country, also known as river crab, widely distributed in lakes in the north and south of the river in my country. Precocious crab refers to the crab species cultivated in that year with larger individuals and a body weight of more than 20g, and the gonads have matured. About 20% of the crab species cultivated in the pond were precocious crab species. If precocious crabs are used to breed commercial crabs , the mortality rate can reach 60%-90%, bringing heavy losses to the vast number of crab farmers. The current method of identifying precocious crabs is generally to look at the appearance. There is indeed a slight difference in appearance between prec...

AI Technical Summary

Problems solved by technology

Early-maturing crabs refer to crab species cultivated in the same year that are relatively large and weigh more than 20g. The gonads have matured. About 20% of the crab species cultivated in the pond in that year are precocious crab species. If early-maturing crabs are used to breed commercial crabs , the mortality rate can reach 60%-90%, which brings heavy losses to the majority of crab farmers.

The current method of distinguishing early-maturing crabs is generally to look at the appearance. There is indeed a slight difference in appearance between early-maturing crabs and normal Chinese mitten crabs, but the contrast is not obvious, and the appearance method must be that the Chinese mitten crabs have grown to a certain extent It can be seen that this is a waste of a lot of manpower and material resources

If the early-maturing crabs cannot be distinguished well, it will greatly affect the development prospects of Chinese mitten crab culture in my country, so there is an urgent need to develop an identification technology for effectively distinguishing early-maturing crabs in this field.

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