Human serum adiponectin detection kit based on micro-particle chemiluminescence immune assay

Abstract

The invention discloses a human serum adiponectin detection kit based on micro-particle chemiluminescence immune assay, and relates to the chemiluminescence detection kits. The human serum adiponectin detection kit comprises reaction buffer liquor, magnetic micro-particles coated with an adiponectin monoclonal antibody, the adiponectin monoclonal antibody marked with acridinium ester, luminescence liquor A, luminescence liquor B, an adiponectin antigen standard substance solution and concentrated scrubbing solution. A preparation method for the human serum adiponectin detection kit comprises the following steps: preparing the reaction buffer liquor; preparing the magnetic micro-particles coated with the adiponectin monoclonal antibody; preparing the adiponectin monoclonal antibody marked with acridinium ester; preparing the luminescence liquor; and preparing the scrubbing solution. According to the human serum adiponectin detection kit, the magnetic micro particles are coupled with the monoclonal antibody, and the micro particles can be suspended in a sample solution, so that the antibodies on the surfaces of the micro particles are sufficiently in contact with a to-be-detected object, and the reaction time is shortened; and the acridinium ester is used as the marker, so that a stable and high-strength signal can be obtained. The human serum adiponectin detection kit is simple to operate, is high in sensitivity, is wide in linearity range, is stable in result, is good in safety and is convenient for automation.

Description

technical field [0001] The invention relates to a chemiluminescence detection kit, in particular to a human serum adiponectin detection kit based on microparticle chemiluminescence immunoassay. Background technique [0002] Adiponectin, also known as Acrp30, GBP28 or AdipoQ, is a hormone protein secreted by adipocytes, which can exist stably in human plasma, with a concentration range of about 2-30 μg / mL, accounting for 0.01% of the total plasma protein . ([1] AritaY, KiharaS, OuchiN, et al. Paradoxicaldecreaseofanadipose-specificprotein, adiponectin, inobesity[J]. BiochemBiophysResCommun, 1999, 257(1): 79-83). With the deepening of research on adiponectin, it has been found that the concentration of adiponectin in the serum of obese people and type II diabetic patients is significantly lower than that of non-obese people, and the reduction of its concentration leads to insulin resistance and hyperinsulinemia , and adiponectin can prevent the formation of atherosclerotic p...

AI Technical Summary

Problems solved by technology

[0005] At present, the microparticle chemiluminescence immunoassay has been widely used in the detection of various antigens such as hepatitis B two-and-a-half (Chinese patent CN102072957), sex hormones (Chinese patent CN103954779), tumor markers, etc., and has achieved good social benefits, but has not yet been seen at home and abroad. With the advent of this type of adiponectin detection kit, the present invention will fill this gap, and its market application prospect is broad

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