A preparation method of low molecular weight barley β-glucan with auxiliary hypoglycemic effect

A low-molecular-weight, glucan technology, which is applied in the direction of food ingredients, food ingredient functions, fermentation, etc., can solve the problems of high molecular weight products, research and confirmation of the hypoglycemic effect of barley β-glucan, and achieve convenient application , good effect of auxiliary hypoglycemic effect

Active Publication Date: 2019-01-11
劲牌持正堂药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to aim at the current application of highland barley β-glucan is limited to low-end products, no one has further studied the hypoglycemic effect of highland barley β-glucan and confirmed its effective molecular weight range, and the conventional method prepared The molecular weight of the product is relatively large, and it is difficult to apply it in products. To provide a method for preparing low-molecular-weight barley β-glucan with auxiliary hypoglycemic effect

Method used

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  • A preparation method of low molecular weight barley β-glucan with auxiliary hypoglycemic effect
  • A preparation method of low molecular weight barley β-glucan with auxiliary hypoglycemic effect
  • A preparation method of low molecular weight barley β-glucan with auxiliary hypoglycemic effect

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Experimental program
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Effect test

Embodiment 1

[0026] see figure 1 , (1) raw material processing: get dry highland barley rice 60kg, be ground into 50 purpose coarse powders after weighing, drop in the extraction tank;

[0027] (2) Extraction: Stir 180L of pure water, put it into the extraction tank, replenish the water to 540L, adjust the pH to 10.0 with saturated sodium carbonate solution, stir and extract at 75°C for 1.5 hours; after the extraction is completed, pour the extract into the holding cylinder , adjusting the pH of the extract to 4.5 with a hydrochloric acid solution with a mass fraction of 10%, stirring evenly, and standing overnight to cool;

[0028] (3) Centrifugation: Centrifuge with a three-legged desktop centrifuge at a speed of 3000r / min, and merge the centrifuged supernatant;

[0029] (4) Enzymatic hydrolysis: ① Amylase enzymatic hydrolysis: add 240g α-amylase to the supernatant, activate at 60°C for 30 minutes, adjust the pH to 5 with saturated sodium carbonate solution, and stir at 50°C for 60 minu...

Embodiment 2

[0036] (1) raw material processing: get dry highland barley rice 30kg, weigh and pulverize into 20 mesh coarse powder, drop into extraction tank;

[0037] (2) Extraction: Stir 90L of pure water, put it into the extraction tank, replenish the water to 270L, adjust the pH to 9.0 with saturated sodium carbonate solution, stir and extract at 75°C for 1.5 hours; after the extraction is completed, pour the extract into the holding cylinder , adjusting the pH of the extract to 4.5 with a hydrochloric acid solution with a mass fraction of 10%, stirring evenly, and standing overnight to cool;

[0038] (3) Centrifugation: centrifuge with a three-legged desktop centrifuge at a speed of 1500r / min, and merge the centrifuged supernatant;

[0039](4) Enzymatic hydrolysis: ① Amylase enzymatic hydrolysis: add 60g α-amylase to the supernatant, activate at 50°C for 30 minutes, adjust the pH to 5.0 with saturated sodium carbonate solution, and stir at 50°C for 40 minutes; ② Glucoamylase enzymatic...

Embodiment 3

[0045] (1) raw material processing: get dry highland barley rice 50kg, weigh and pulverize into 30 purpose coarse powder, drop into extraction tank;

[0046] (2) Extraction: Stir 150L of pure water, put it into the extraction tank, add water to 450L, adjust the pH to 10.0 with saturated sodium carbonate solution, stir and extract at 85°C for 2 hours; after the extraction is completed, pour the extract into the holding cylinder , adjusting the pH of the extract to 6.5 with a hydrochloric acid solution with a mass fraction of 10%, stirring evenly, and standing overnight to cool;

[0047] (3) Centrifugation: Centrifuge with a three-legged desktop centrifuge at a speed of 3000r / min, and merge the centrifuged supernatant;

[0048] (4) Enzymatic hydrolysis: ① Amylase enzymatic hydrolysis: add 150g α-amylase to the supernatant, activate at 60°C for 30 minutes, adjust the pH to 6.5 with saturated sodium carbonate solution, and stir at 60°C for 50 minutes; ② Glucoamylase enzymatic hydr...

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Abstract

The invention discloses a method for preparing low-molecular-weight highland barley beta-glucan with a hyperglycemic assistance effect. The method comprises the following steps: firstly, grinding highland barley to be 10-50 meshes, adding water to regulate the pH to be 8-10, extracting for 1-2 hours, regulating the pH back to 2.5-6.5, centrifuging, and adding alpha-amylase which is 0.1-0.4 percent of total weight of the materials to perform enzymolysis for 40-60 minutes; adding 0.13-0.4 percent of total weight of the materials to perform enzymolysis for 40-60 minutes; deactivating enzyme, centrifuging, concentrating, precipitating with ethanol and drying to obtain beta-glucan having a number-average molar mass of 6.827*10<2>-7.968*10<4>. In the product, the content of total polysaccharides is more than or equal to 50 percent, the content of beta-glucan is more than or equal to 15 percent, and the dissolved load in water and 50-degree ethanol is more than or equal to 150mg / L. The low-molecular-weight highland barley beta-glucan has a good hyperglycemic assistance effect, and can be conveniently applied to various functional foods.

Description

technical field [0001] The invention provides a method for preparing low-molecular-weight barley β-glucan with the effect of assisting in lowering blood sugar. The number-average molecular weight (Mn) of the barley β-glucan prepared by the invention is 6.827×10 2 ~7.968×10 4 g / mol, as verified by pharmacodynamic tests, the low-molecular-weight highland barley β-glucan prepared by the method of the present invention has a good auxiliary hypoglycemic effect, and is more convenient to be applied in various functional foods. Background technique [0002] Highland barley belongs to Poaceae, barley is one of the oldest food crops in the world, and it is also livestock feed and industrial raw material crops. Highland barley is the crop with the highest β-glucan content among wheat crops in the world. According to the test, the average content of β-glucan in highland barley is 6.57%, and the content of β-glucan in fine varieties can even reach 8.6%. Highland barley has received gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A23L29/30C12P19/20C12P19/08
CPCA23V2002/00C08B37/0003C08B37/0024C12P19/08C12P19/20A23V2200/326
Inventor 胡辉龙林梅菊陈志元杨跃军刘源才
Owner 劲牌持正堂药业有限公司
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