DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane) degradation bacteria and in-situ remediation method using DDT for contaminated soil
A technology for contaminated soil and in situ remediation, applied in the restoration of contaminated soil, microorganism-based methods, biochemical equipment and methods, etc. High efficiency, short cycle and other problems, to achieve the effect of efficient degradation of DDT, simple culture process and low cost
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Embodiment 1
[0019] Example 1: Isolation and Identification of Arthrobacterglobiformis DC-1 Strain and Its Degradation Performance to DDT
[0020] The microbial source was collected from a farmland polluted by DDT for a long time in Shenbei New District, Shenyang City. The surface soil (0-20cm) was collected at multiple points, and the residues such as plant roots and gravel were removed, and then refrigerated and transported back to the laboratory.
[0021] Screening, isolation and purification of highly efficient degrading bacteria: Weigh 2g of collected soil samples and inoculate them into 100ml of fresh liquid inorganic salt medium containing 100mg / Lp,p'-DDT (the composition and content of the inorganic salt medium are as follows: KH 2 PO -4 1.0g, MgSO 4 ·7H 2 O0.5g, NH 4 NO 3 1.0g, K 2 HPO 4 1.0g, CaCl 2 0.02g, FeCl 2 0.005g, 1000ml of distilled water, PH=7.0, 121℃ high pressure steam sterilization for 20min), shake culture at 30℃, 150r / min shaker for 4 days, then transfer 1ml...
Embodiment 2
[0031] Example 2: Growth and degradation performance test of Arthrobacter globiformis DC-1 strain.
[0032] Add 5ml of basal medium to a sterilized test tube, add 10mg·L -1 DDT was used as a carbon source, and quantitatively inoculated with 0.5 mL of DC-1 strain suspension (bacterial concentration OD 600 =1.0), at 30°C, 160r·min -1 Shake the culture in the dark, and take samples after 1d, 3d, 5d, 7d, and 10d to measure the growth amount of strain DC-1 and the degradation rate of DDT. At the same time, set a control without adding bacteria, and set 3 replicates for each sample. The results are shown in Table 2.
[0033] Table 2 DDT degradation rate and strain DC-1 growth
[0034]
[0035] from Table 2 and figure 2 It can be seen that the strain DC-1 can grow with DDT as the sole carbon source, and has the ability to degrade DDT efficiently within 24 hours, with a degradation rate as high as 76.29%, OD 600 Also reached the maximum value, compared with the existing DDT-d...
Embodiment 3
[0036] Example 3: In-situ remediation of DDT-contaminated farmland soil by Arthrobacterglobiformis DC-1 strain
[0037] The test was carried out in a farmland polluted by DDT for a long time in Shenbei New District, Shenyang City. Fresh soil samples of 0-20 cm in the test field were discarded from impurities such as weeds, stones, plastics, etc., mixed thoroughly and leveled, and the soil physical and chemical properties were measured. The properties are: the organic matter content is 54.37g·kg -1 , the cation exchange capacity is 13.09cmol kg -1 , the pH value is 7.17, the proportions of clay, silt and sand are 26.11%, 72.82% and 1.07% respectively, and the soil type is silty clay. The experimental field was divided into plots with an area of 1.0m×1.0m, and the interval between adjacent restoration units was about 20cm. Two treatments, blank control (CK) and soil application of DDT-degrading bacteria (DC-1), were set up, and each treatment was set up 3 times. repeat. 500...
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