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Infection preventing and osteoblast differentiation promoting antibacterial peptide modified titanium alloy prosthesis and making method thereof

An osteogenic differentiation, titanium alloy technology, applied in the direction of prosthesis, pharmaceutical formulation, medical science, etc., can solve the problems of loosening and infection, and achieve the effect of low cost, good repeatability and excellent antibacterial biological activity.

Inactive Publication Date: 2016-08-03
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an antibacterial peptide modified titanium alloy prosthesis that resists infection and promotes osteogenic differentiation and its preparation method in view of the two major complications of infection and loosening that may occur in the implantation of titanium alloy prostheses at present. and use

Method used

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  • Infection preventing and osteoblast differentiation promoting antibacterial peptide modified titanium alloy prosthesis and making method thereof
  • Infection preventing and osteoblast differentiation promoting antibacterial peptide modified titanium alloy prosthesis and making method thereof
  • Infection preventing and osteoblast differentiation promoting antibacterial peptide modified titanium alloy prosthesis and making method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of the antimicrobial peptide-modified titanium alloy prosthesis of the present invention (1)

[0037] 1. Preparation of antimicrobial peptide KR-12

[0038] Antibacterial peptide KR-12 was synthesized by solid-phase synthesis, and its amino acid sequence was: KRIVQRIKDFLR (SEQ ID NO. 1). The purity of KR-12 antimicrobial peptide is above 95%.

[0039] 2. Introduction of functional functional group amino group on the surface of titanium sheet

[0040]A functional functional group amino group was introduced on the surface of the titanium sheet by a two-step method. First, the titanium sheet was subjected to alkali treatment to introduce a hydroxyl group, and then a silanization treatment was performed to introduce an amino group. Specifically: first, immerse a titanium alloy disc (Ti6Al4V, Ti, φ10×2mm, Shanghai Yan Titanium Metal Materials Co., Ltd.) into a beaker containing a strong alkali solution with a concentration of 5M, seal the beaker with ...

Embodiment 2

[0060] Example 2 Preparation of the antimicrobial peptide-modified titanium alloy prosthesis of the present invention (2)

[0061] 1. Preparation of antimicrobial peptide KR-12

[0062] Same as Example 1.

[0063] 2. Introduction of functional functional group amino group on the surface of titanium sheet

[0064]A functional functional group amino group was introduced on the surface of the titanium sheet by a two-step method. First, the titanium sheet was subjected to alkali treatment to introduce a hydroxyl group, and then a silanization treatment was performed to introduce an amino group. Specifically: first, immerse a titanium alloy disc (Ti6Al4V, Ti, φ10×2mm, Shanghai Yan Titanium Metal Materials Co., Ltd.) into a beaker containing a strong alkali solution with a concentration of 10M, seal the beaker with plastic wrap, and oil it at 60°C. Bath reaction 8h. Then take out the titanium sheet and ultrasonically clean it twice with deionized water for 2 min each time. After ...

Embodiment 3

[0067] Example 3 Preparation of the antimicrobial peptide-modified titanium alloy prosthesis of the present invention (3)

[0068] 1. Preparation of antimicrobial peptide KR-12

[0069] Same as Example 1.

[0070] 2. Introduction of functional functional group amino group on the surface of titanium sheet

[0071] A functional functional group amino group was introduced on the surface of the titanium sheet by a two-step method. First, the titanium sheet was subjected to alkali treatment to introduce a hydroxyl group, and then a silanization treatment was performed to introduce an amino group. Specifically: first, immerse a titanium alloy disc (Ti6Al4V, Ti, φ10×2mm, Shanghai Yan Titanium Metal Materials Co., Ltd.) into a beaker containing a strong alkali solution with a concentration of 8M, seal the beaker with plastic wrap, and oil at 70 ° C Bath reaction for 10h. Then take out the titanium sheet and ultrasonically clean it twice with deionized water for 2 min each time. Aft...

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Abstract

The invention relates to an anti-infection and osteogenic differentiation-modified titanium alloy prosthesis and a preparation method thereof. The present invention fixes KR-12, a biologically active fragment of human antimicrobial peptide LL-37, on a titanium plate by means of covalent grafting, and the KR-12 antimicrobial peptide fixed on the titanium plate can maintain its antibacterial biological activity and can Promote osteogenic differentiation and extracellular matrix mineralization of bone marrow mesenchymal stem cells. The invention has simple process and low production cost, but at the same time solves the two major complications of infection and loosening that traditional orthopedic prostheses face in clinical application, and has very important significance in the field of titanium alloy biofunctionalization.

Description

technical field [0001] The invention relates to the technical field of orthopedic prostheses, in particular to an antimicrobial peptide-modified titanium alloy prosthesis that resists infection and promotes osteogenic differentiation and a preparation method thereof. Background technique [0002] The two most important complications of orthopaedic prostheses are infection and loosening. Infection is mainly caused by bacteria easily adhering to the surface of the prosthesis and forming biofilm, while loosening is mainly caused by poor osseointegration of bone-prosthesis. At present, the construction of anti-infective implants is mainly based on the theory of anti-bacterial adhesion and anti-biofilm formation, ignoring the problem of poor osseointegration, while osseointegrated prosthesis ignores the problem of anti-infection. For example, antibacterial coatings of anti-infective implants produce antibacterial effects by releasing preloaded antibacterial agents such as antibio...

Claims

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Application Information

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IPC IPC(8): A61L27/06A61L27/54C23C22/54
CPCA61L27/06A61L27/54A61L2300/252A61L2300/404A61L2400/18A61L2430/02C23C22/54
Inventor 岳冰聂彬恩敖海勇
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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