Application of Rice Thermosensitive Male Sterility Gene tms10 and Fertility Restoration Method

Abstract

The invention relates to an application of a paddy rice temperature sensitive male sterility gene TMS10 and a fertility restoration method. The amino acid sequence of TMS10 is represented by SEQ ID No.1. A conventional method is adopted to knock, change or inhibit TMS10 gene to reduce the expression level of TMS10 gene in common paddy rice species so as to obtain paddy rice male sterility line. The prepared paddy rice sterility line does not have abnormal phenotype during the vegetative growth phase; anther development is abnormal during the reproductive stage; the expressions of the phenotype are the same in the mutants of non-glutinous rice and japonica rice; the paddy rice is sterile at an average temperature of 28 DEG C, and paddy rice is fertile at an average temperature of 22 DEG C. The temperature sensitive characteristic, which is utilized during the process of two line hybrid seed production, is widely used in agricultural production.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to the application of a rice temperature-sensitive male sterility gene TMS10 and a method for restoring fertility. Background technique [0002] Rice is one of the world's major food crops, feeding the world's rice-based population. Therefore, improving rice yield and quality has become a national economy and people's livelihood issue. Since the first green revolution, along with the application of dwarf varieties, three-line hybrid seed production and two-line hybrid seed production have successively become important factors for increasing production. At present, the methods of hybrid rice breeding commonly used in production include three-line hybrid rice and two-line hybrid rice. The three lines include sterile line, maintainer line and restorer line. Three-line seed production has indeed provided a large number of excellent varieties, but in practice, the re...

AI Technical Summary

Problems solved by technology

Three-line seed production has indeed provided a large number of excellent varieties, but in practice, the reproduction and preservation of each line requires a lot of manpower and financial resources

However, the number of genes controlling photothermosensitive male sterility that has been reported so far is still very small, so the discovery and research of new photothermosensitive sterile materials provides more clues and the possibility of increasing production for breeding work

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