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Method for separating procyanidin polymer and oligomer from degreased sea buckthorn seeds

A technology of proanthocyanidins and seabuckthorn seeds, applied in organic chemistry and other fields

Active Publication Date: 2016-08-10
BEIJING POWDERY FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is that the seabuckthorn seed proanthocyanidin product obtained by the existing extraction technology is a mixture of high polymer and oligomer, which affects product performance, and The process of crude extraction and subsequent separation of proanthocyanidin oligomers from seabuckthorn seeds is complex and costly

Method used

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  • Method for separating procyanidin polymer and oligomer from degreased sea buckthorn seeds

Examples

Experimental program
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Embodiment 1

[0033] The specific operation steps are:

[0034] 1) Alcohol extraction: extract defatted seabuckthorn seeds with ethanol aqueous solution, and filter to obtain a crude extract. The volume concentration of ethanol aqueous solution is 10-90%, preferably 50-70%, and the weight ratio of the extracted material to liquid is 1:2-15 , the extraction temperature is 10~60℃, the number of extractions is 1~3 times, and the time of each extraction is 0.5~24h. The auxiliary method can be mechanical stirring or ultrasonic treatment;

[0035] 2) Concentration: The crude extract obtained in step 1) is concentrated under reduced pressure, and the ethanol is removed to obtain a concentrated solution. Brix value is 10~30;

[0036] 3) Heat sink: Stir the concentrated solution obtained in step 2) at 30~60°C for 10min~60min, keep it warm for 10min~60min, and separate the precipitate and supernatant;

[0037] 4) Cold precipitation: cool down the supernatant obtained in step 3) to 1~15°C, keep it ...

Embodiment 2

[0040] Optimized on the basis of Example 1.

[0041] The defatted seabuckthorn seeds described in this embodiment are the leftovers after extracting seabuckthorn seed oil, that is, defatted seabuckthorn seed dregs, and the method for separating proanthocyanidin high polymers and oligomers from defatted sea buckthorn seeds described in this embodiment,

[0042] Including the following steps:

[0043] 1) 3 kg of defatted seabuckthorn seeds, according to the weight ratio of material to liquid 1:3, add ethanol aqueous solution with a volume concentration of 60% and stir for extraction. The extraction temperature is 60°C, the number of extractions is 3 times, and the extraction time for each extraction is 0.5h. Ultrasonic-assisted extraction is used for each extraction, the ultrasonic power is 200W, the ultrasonic frequency is 20kHz, and the stirring speed is 30r / min;

[0044] 2) Concentrate under reduced pressure to remove alcohol, the temperature is 35°C, the absolute pressure i...

Embodiment 3

[0049] Optimized on the basis of Example 1.

[0050] The defatted seabuckthorn seeds described in this embodiment are leftovers after extracting seabuckthorn seed oil, and the method for separating proanthocyanidin high polymers and oligomers from defatted sea buckthorn seeds described in this embodiment comprises the following steps:

[0051] 1) 56 kg of defatted seabuckthorn seeds, according to the weight ratio of material to liquid 1:7, add ethanol solution with a volume concentration of 60% and soak for 12 hours, the extraction temperature is 10°C, and the extraction process is continuously stirred as auxiliary extraction, and the stirring speed is 60r / min;

[0052] 2) Concentrate under reduced pressure to remove alcohol, the temperature is 40°C, the absolute pressure is 0~1000Pa, and the Brix value of the concentrated solution is monitored online. When the Brix value of the concentrated solution is 17, the concentration is stopped and the material is discharged while it is...

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Abstract

The invention discloses a method for separating a procyanidin polymer and oligomer from degreased sea buckthorn seeds. The method comprises: extracting degreased sea buckthorn seeds with an ethanol aqueous solution and performing filtration to obtain a coarse extracted liquid; performing vacuum concentration to remove ethanol from the coarse extracted liquid, and obtaining a concentrated liquid; stirring the concentrated liquid at 30-60 DEG C, performing temperature preservation, allowing the concentrated liquid to stand, and performing separation to obtain a precipitate and a supernatant; reducing the temperature of the supernatant to 1-15 DEG C, performing temperature preservation, allowing the supernatant to stand, and performing secondary separation to obtain a precipitate and a supernatant; drying the precipitate obtained from secondary separation to obtain a procyanidin polymer extracted from sea buckthorn seeds; and concentrating the supernatant obtained from secondary separation to obtain a procyanidin oligomer extracted from sea buckthorn seeds. According to the method, the procyanidin polymer and oligomer can be fully extracted from the degreased sea buckthorn seeds. The method is simple and is low in cost.

Description

technical field [0001] The invention relates to the technical field of separation and extraction of anthocyanins, in particular to the separation and extraction of anthocyanins from seabuckthorn, in particular to a method for separating proanthocyanidin high polymers and oligomers from defatted sea buckthorn seeds, which belongs to the extraction and separation technology of plant effective components field. Background technique [0002] Seabuckthorn seed proanthocyanidins are a class of natural active substances present in seabuckthorn seeds, which have anti-oxidation, free radical scavenging, anti-mutation, anti-cancer, anti-inflammatory, anti-allergic, anti-virus, lowering blood lipids, inhibiting platelet aggregation, anti-thrombosis, It has a wide range of health pharmacological effects such as improving blood sugar and enhancing immunity. Seabuckthorn seed proanthocyanidins are the general term for seabuckthorn seed proanthocyanidins with different degrees of polymeri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 谭志超张存存陈华高彦祥王辉斌
Owner BEIJING POWDERY FOOD
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