Compound with anti-candida albicans activity and its preparation method and application
A technology of Candida albicans and compounds, applied in the field of compounds with anti-Candida albicans activity and its preparation, can solve the problems of limited application, limited price and high price, and achieve the effect of wide development and application prospects
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Embodiment 1
[0044] Example 1 , compound preparation
[0045] 1.1. Fermentation
[0046] Streptomyces lavenduligriseus CGMCC No. 10249 was inoculated into the seed medium, cultured on a shaker at 28°C and 220r / min for 2 days, and then the cultured seeds were inoculated with 8% of the inoculum. In the fermentation medium in a 5L fermentor, culture at 26-28°C for 5-7 days.
[0047] 1.2. Separation and purification
[0048] The fermentation product obtained in Example 1.1 was extracted with methanol, and the extract was obtained by distillation under reduced pressure. The obtained extract was extracted with n-butanol, and the extract was obtained by distillation under reduced pressure. The obtained extract is then separated by silica gel column chromatography with a 200-300 mesh silica gel column, and chloroform and methanol are used as elution solvents for gradient elution, wherein the percentage of methanol is 0%, 10%, 20%, 30%, 40%, 50% and 100% were concentrated under reduced pressu...
Embodiment 2
[0049] Example 2. Structure identification of compound 1
[0050] The compound 1 obtained in Example 1.2 was detected by positive ion electrospray mass spectrometry, and its spectrum was as follows figure 1 It shows that its quasi-molecular ion peak is: m / z 671.4010[M+H] + , the molecular formula is C35 H 58 O 12 , the hydrogen spectrum of the sample was measured by Bruker Avance II-400 superconducting NMR instrument figure 2 ), carbon spectrum ( image 3 ) and related two-dimensional spectra (HMQC spectrum, H-HCOSY spectrum, HMBC spectrum, Figure 4~6 ), and the NMR data are shown in Table 1.
[0051] Table 1. NMR data table of compound 1 (CD 3 OD , , 400MHz)
[0052] position δ H
δ C
HMBC 1 173.0 2 2.44,t(11.9) 59.6 2’,4 3 4.23,t(13.1) 72.7 2,4 4a 1.46-1.50,m 42.5 4b 1.37-1.40,m 5 4.06-4.10,m 72.3 6 1.61-1.68,m 44.7 7 4.06-4.10,m 73.0 8 1.46-1.50,m 45.1 9 4.06-4.10,m...
Embodiment 3
[0057] Example 3. Structure identification of compound 2
[0058] The compound 2 obtained in Example 1.2 was detected by positive ion electrospray mass spectrometry, and its spectrum was as follows Figure 7 It shows that its quasi-molecular ion peak is: m / z 751.4241[M+Na] + , the molecular formula is C 38 H 64 O 13 , the hydrogen spectrum of the sample was measured by Bruker Avance II-400 superconducting NMR instrument Figure 8 ), carbon spectrum ( Figure 9 ) and related two-dimensional spectra (HMQC spectrum, H-HCOSY spectrum, HMBC spectrum, Figure 10-12 ), and the NMR data are shown in Table 2.
[0059] Table 2. NMR data table of compound 2 (CD 3 OD , , 400MHz)
[0060] position δ H
δ C
HMBC 1 173.0 2 2.73 60.4 2’,4 3 4.36 73.2 2,4 4 1.68 41.3 5 4.18 73.9 6a 1.73 45.3 6b 1.80 7 4.18 73.8 8 1.64 45.3 9 4.18 74.1
[0061] 10a 1.46 45.2 10b ...
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