Shewanella frigidimarina strain and method for producing exopolysaccharide

A cold sea Shewanella, Shewanella technology, applied to microbial strains and its application fields, to achieve the effect of promoting intestinal health and reducing feed costs

Active Publication Date: 2016-08-10
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant reports on the application of Antarctic ex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] (2) Preparation of fermentation broth: inoculate Shewanella coldsea W32-2 activated in step (1) into 2216E medium for fermentation and culture, the inoculation amount is 1-10%, 150-200 rpm, and the temperature is set at 8-15°C; ferment for 4-7 days to obtain the required fermentation liquid;

[0028] (3) Bacteria removal: take the fermentation broth obtained in step (2) and centrifuge at 8000-10000rpm for 5-10min, and absorb the fermentation supernatant for later use;

[0029] (4) Separation and purification of exopolysaccharides: ethanol precipitation of the fermentation supernatant obtained in step (3), adding absolute ethanol 3 times the volume of the fermentation supernatant, precipitation for 3-6 hours, centrifugation at 5000-10000rpm for 5-10min, pouring Remove the supernatant, dissolve the precipitated polysaccharide with deionized water according to the volume of the fermentation supernatant, and use the Sevag method to remove the protein, repeat 3-5 times, and ...

Embodiment 1

[0035](1) Activation of the above-mentioned Shewanella coldsea strain W32-2: take the above-mentioned Shewanella coldsea strain W32-2 and inoculate it into the inclined 2216E medium, and cultivate it at 10°C 5 days; use an inoculation loop to inoculate the slant cultured Shewanella coldsea strain W32-2 onto 10 mL of 2216E medium, and activate the culture at 10°C and 150 rpm for 5 days;

[0036] (2) Preparation of fermentation broth: Inoculate Shewanella coldsea W32-2 activated in step (1) into 1L 2216E medium for fermentation and culture, with an inoculum size of 5%, 150 rpm, and a temperature of 10°C; Fermentation 5 days then obtains required fermented liquid;

[0037] (3) Bacteria removal: take the fermentation broth obtained in step (2) and centrifuge at 8000rpm for 10min, and absorb the fermentation supernatant for later use;

[0038] (4) Separation and purification of exopolysaccharides: ethanol precipitation of the fermentation supernatant obtained in step (3), adding a...

Embodiment 2

[0041] (1) Activation of the Shewanella coldsea strain W32-2: Take the Shewanella coldsea strain W32-2 described in and inoculate it into the slant 2216E medium, and culture it at 15°C 7 days; use an inoculation loop to inoculate Shewanella coldsea strain W32-2 on 10 mL of 2216E medium after slant culture, and activate culture at 15°C and 180rpm for 7 days;

[0042] (2) Preparation of fermentation broth: Inoculate Shewanella coldsea W32-2 activated in step (1) into 1L 2216E medium for fermentation and culture, with an inoculum size of 10%, 180 rpm, and a temperature of 15°C; Fermentation 7 days then obtains required fermented liquid;

[0043] (3) Bacteria removal: take the fermentation liquid obtained in step (2) and centrifuge it at 10,000 rpm for 5 minutes, and absorb the fermentation supernatant for later use;

[0044] (4) Separation and purification of exopolysaccharides: ethanol precipitation of the fermentation supernatant obtained in step (3), adding absolute ethanol 3...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses shewanella frigidimarina and a method for producing exopolysaccharide. The strain W32-2(shewanella frigidimarina) capable of being used for high-yield production of exopolysaccharide is separated and screened from gravel and sediment samples of the South Pole, and preserved in China General Microbiological Culture Collection Center on Feb 01, 2016 with the preservation number of CGMCC No.12129. A polar region source exopolysaccharide product with antioxidant activity and probiotic activity is prepared by means of the strain W32-2(shewanella frigidimarina), and the product is easy to prepare and has broad market prospects.

Description

technical field [0001] The present invention relates to a kind of microorganism bacterial strain and application thereof, relate in particular to a kind of Shewanella cold sea strain ( Shewanella frigidimarina ) W32-2 and the production method of exopolysaccharide thereof. Background technique [0002] With the rapid development of aquaculture, especially high-density farming, the prevention and control of aquaculture diseases is often an important factor in determining economic benefits. With the introduction of relevant policies by the Ministry of Agriculture, the application of antibiotics in aquaculture will gradually be replaced, and the prevention of aquaculture diseases is gradually being valued by people. "Prevention is more important than cure" has become the mainstream concept of aquaculture. How to effectively prevent aquaculture diseases and at the same time improve economic benefits and realize green ecological farming is a bottleneck problem in the aquacultu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12P19/04A23K20/163A23K50/80C12R1/01
CPCC12P19/04C12N1/205C12R2001/01
Inventor 徐长安高培丽黄仕新唐旭刘源森林凌
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap