Method for constructing tissue engineered cartilages in vivo

A tissue engineering and cartilage technology, applied in the field of biological tissue, can solve the problems of poor mechanical strength and no specific shape of cartilage, and achieve the effect of improving the success rate, avoiding adverse reactions, and facilitating the precise control and maintenance of shape.

Inactive Publication Date: 2016-08-17
SHANGHAI TISSUE ENG LIFE SCI
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Problems solved by technology

However, the resulting cartilage has no specific shape and poor mecha...

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  • Method for constructing tissue engineered cartilages in vivo

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Embodiment

[0048] S1. Isolation and culture of sheep ear chondrocytes;

[0049] S1.1. Aseptically cut goat ear cartilage pieces about 2cm×2cm in size, and cut them into 1mm pieces 3 Cartilage blocks were washed repeatedly with chloramphenicol solution, added 3 times the volume of 0.25% trypsin, placed in a constant temperature water bath shaker at 37°C for digestion for 30 minutes to remove fibrous connective tissue outside the cartilage tissue, and then washed with phosphate buffered saline (PBS ) was washed 3 times, the cartilage pieces were precipitated, and trypsin was removed;

[0050] S1.2. Add 0.15% type II collagenase, prepare the corresponding concentration with chondrocyte culture medium, filter with a needle filter of 0.22 microns, use immediately, and digest in a constant temperature water bath shaker at 37°C for 6-8 hours;

[0051] S1.3. Until most of the cartilage pieces are digested, the obtained digestion solution is filtered with a 100-micron cell strainer, centrifuged ...

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Abstract

The invention discloses a method for constructing tissue engineered cartilages in vivo, which comprises the following steps: carrying out multiplication culture on a great deal of auricular cartilage cells in vitro; preparing a stratified cartilage cell membrane; preparing a PLC (three-dimensional printing) inner core with a special form; preparing a sandwich mode of the stratified cartilage cell membrane and a material; and performing an operation for implanting a compound containing the membrane and the inner core into animal muscle. According to the method, a three-dimensional printed material is used as an inner core scaffold, and the material is coated by the cell membrane to prevent the scaffold material from being in direct contact with in vivo tissue, thereby reducing the inflammatory reaction caused by the scaffold material in vivo and increasing the success ratio of cartilage regeneration in vivo. In addition, through three-dimensional printing, inner core materials with various special forms can be manufactured, and tissue engineered cartilages corresponding to the inner core materials are constructed; and meanwhile, by adjusting the height of a printing layer, the density of grids and the mode of wiring, due to different superimposed layers of the membrane, the constructed cartilages have different mechanical strength which can reach a relatively high level.

Description

technical field [0001] The invention relates to the field of biological tissues, in particular to a method for constructing tissue-engineered cartilage with a special shape in vivo by applying a multilayer chondrocyte membrane technology combined with a PCL core material. Background technique [0002] At present, the construction of tissue-engineered cartilage with a large area and certain mechanical strength in large animals has become a major problem and cannot meet certain clinical needs, such as the repair of long-segment tracheal defects. The rise and rapid development of tissue engineering technology in recent years has provided a new idea, which uses autologous cells and material scaffolds to pre-build tissue-engineered cartilage with a specific shape. However, the existing methods for constructing tissue engineered cartilage have not yet achieved breakthroughs due to many problems in effective shaping, mechanical strength and immune response. [0003] Existing studi...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/18A61L27/58A61L27/50
CPCA61L27/3817A61L27/18A61L27/3852A61L27/50A61L27/58A61L2430/06C08L67/04
Inventor 曹谊林周广东李丹殷宗琦刘浥刘豫
Owner SHANGHAI TISSUE ENG LIFE SCI
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