Method for culturing Isaria cicadae Miquel by using Tenebrio molitor pupae
A technology of Tenebrio molitor and I. clavulata, which is applied in the field of microbial biology, can solve problems such as large market demand, unsatisfied demand, and shortage of wild cicadae resources, achieving good results and easy operation.
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Embodiment 1
[0016] (1) Preparation of conidia suspension of Corydalis cicadae: Corydalis cicadae strains were inoculated on PDA plate medium, cultured at 28°C for 10 days, washed with sterile water, and diluted to adjust the final concentration of spores up to 5×10 7 pcs / ml;
[0017] (2) Preparation of aseptic Tenebrio molitor pupa: 35 robust aseptic Tenebrio larvae were selected and cultured in a sterilized bran culture bottle, which was filled with 60 g of bran in a 100 ml canning bottle, Routine sterilization for 30 min; after the Tenebrio larvae pupate, select the Tenebrio molitor pupae within 8 hours of pupation, pick them out with sterile tweezers, and put them into sterile empty canned bottles for later use;
[0018] (3) Tenebrio molitor pupae infected by Trichosporium cicadas: Use sterile tweezers to dip the Tenebrio molitor pupae within 8 hours of pupation in the cicada flower conidia suspension to control the formation of Tenebrio molitor pupae immersed in the spore suspension....
Embodiment 2
[0022] (1) Preparation of conidia suspension of Corydalis cicadae: Corydalis cicadae strains were inoculated on PDA plate medium, cultured at 28°C for 15 days, washed with sterile water, and diluted to adjust the final concentration of spores up to 5×10 7 pcs / ml;
[0023] (2) Preparation of aseptic Tenebrio molitor pupa: 35 robust aseptic Tenebrio larvae were selected and cultured in a sterilized bran culture bottle, which was filled with 60 g of bran in a 100 ml canning bottle, Routine sterilization for 30 min; after the Tenebrio larvae pupate, select the Tenebrio molitor pupae within 8 hours of pupation, pick them out with sterile tweezers, and put them into sterile empty canned bottles for later use;
[0024] (3) Tenebrio molitor pupae infected by Trichosporium cicadas: Use sterile tweezers to dip the Tenebrio molitor pupae within 8 hours of pupation in the cicada flower conidia suspension to control the formation of Tenebrio molitor pupae immersed in the spore suspension....
Embodiment 3
[0028] (1) Preparation of conidia suspension of Corydalis cicadae: Corydalis cicadae strains were inoculated on PDA plate medium, cultured at 28°C for 13 days, washed with sterile water, and diluted to adjust the final concentration of spores up to 5×10 7 pcs / ml;
[0029] (2) Preparation of aseptic Tenebrio molitor pupa: 35 robust aseptic Tenebrio larvae were selected and cultured in a sterilized bran culture bottle, which was filled with 60 g of bran in a 100 ml canning bottle, Routine sterilization for 30 min; after the Tenebrio larvae pupate, select the Tenebrio molitor pupae within 8 hours of pupation, pick them out with sterile tweezers, and put them into sterile empty canned bottles for later use;
[0030] (3) Tenebrio molitor pupae infected by Trichosporium cicadas: Use sterile tweezers to dip the Tenebrio molitor pupae within 8 hours of pupation in the cicada flower conidia suspension to control the formation of Tenebrio molitor pupae immersed in the spore suspension....
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