Promoter driving specific expression of amylose synthase gene gbssi-3 in banana fruit and its application

Abstract

The invention provides a promoter for promoting a specific expression of amylose synthetase gene GBSSI-3 in banana fruits and application of the promoter. A nucleotide sequence of the promoter is as shown in SEQ ID NO.1, and the promoter is capable of guiding specific expression of the amylose synthetase gene GBSSI-3 in fruits instead of roots, stems and leaves. By cloning and functional study on the GBSSI-3 promoter, part of functions and promotion activity of the promoter can be determined, and theoretical bases can be provided for researches on the sequence of the banana GBSSI-3 gene promoter; a candidate fruit specific promoter is provided for development and application of tissue specific promoters, increase of amylose content of the banana fruits by means of genetic engineering and improvement of amylose quality; theoretical bases are provided for clarification of a whole starch synthesis and regulation network, and a candidate fruit specific promoter is provided for meeting the requirement on diversification of starch different in straight / branch chains.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to a promoter for driving the specific expression of amylose synthase gene GBSSI-3 in banana fruit and its application. Background technique [0002] Banana is an important economic crop in tropical and subtropical regions, and it is the second largest fruit in the world after citrus. As the main component of banana fruit, starch has prompted it to be identified by the Food and Agriculture Organization of the United Nations (FAO) as the fourth largest food crop after rice, wheat, and corn, and is the ration of more than 400 million people (Englberger et al. ,2006). However, with the rapid development of the banana industry and the rapid improvement of people's living standards, the requirements for the quality of banana fruit are also getting higher and higher. Applying modern biotechnology to banana quality improvement can solve some problems that are difficult...

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Problems solved by technology

[0003] At present, the high-quality banana fruit rate in my country is less than 30%, and most banana fruits have quality problems such as hard core, poor waxiness, low sugar content, and unstable resistant starch content during the post-ripening process.

[0004] Constitutive promoters (such as 35S) are commonly used in plant genetic engineering to regulate the expression of foreign genes, but there are some shortcomings, such as: 35S promoters can promote high expression of foreign genes in dicotyledonous plants, but in monocotyledons The expression ability of driving genes in leaf plants is not strong; the expression driven by 35S has no obvious tissue and organ specificity, and produces a large number of heterologous expression products and even toxic substances, resulting in plant metabolic imbalance affecting its normal growth and development or even death

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