SENP3 gene and use of its expression product as diagnosis and treatment target for esophageal cancer

A gene expression, esophageal cancer technology, applied in the biological field, can solve problems such as little value

Active Publication Date: 2016-08-17
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of abnormally expressed genes and proteins have been found in esophageal cancer tissues, such as DCR3 gene, PCNA, EBP1, STAT3, CYCLIND1, VEG, and esophageal cancer-related gene 4. Therefore, new genes or markers urgently need to be confirmed and found by research. For example, in patients with liver cancer, the detection of AFP in peripheral blood has a high sensitivity. sex

Method used

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  • SENP3 gene and use of its expression product as diagnosis and treatment target for esophageal cancer
  • SENP3 gene and use of its expression product as diagnosis and treatment target for esophageal cancer
  • SENP3 gene and use of its expression product as diagnosis and treatment target for esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Differential expression of SENP3 gene

[0061] 1. Experimental materials:

[0062] The esophageal cancer tissue is the specimen removed by the hospital's thoracic surgery, and the normal tissue is the specimen taken out under the microscope of the gastroscope room. Cut with a scalpel blade.

[0063] All cases were confirmed pathologically. Including 40 cases of esophageal cancer tissue and 30 cases of normal esophageal tissue. Among them, there were 34 cases of esophageal squamous cell carcinoma and 6 cases of esophageal adenocarcinoma. Normal esophageal tissue was used as the control group. All patients were pathologically confirmed before operation, and no radiotherapy, chemotherapy and other treatments were performed before operation, and no tumors in other parts were found. All tissues were stored in liquid nitrogen within half an hour of the operation, and then transferred to a -80°C refrigerator for freezing.

[0064] 2. Acquisition of RNA from eso...

Embodiment 2

[0074] Example 2 Differential expression of SENP3 protein

[0075] 1. The research object is the same as in Example 1.

[0076] 2. Extract tissue total protein

[0077]Follow the instructions of the EpiQuik Tissue / Cell Total Protein Extraction Kit for protein extraction.

[0078] 3. Western blot detection

[0079] The extracted protein was quantitatively subjected to SDS-PAGE electrophoresis, followed by membrane transfer, blocking, primary antibody incubation, secondary antibody incubation, and color development.

[0080] 4. Statistical processing

[0081] The gray value of the protein band was analyzed using Image J software, and the gray value of the SENP3 protein band was normalized with β-actin as an internal reference. The results and data are expressed in the form of mean ± standard deviation, and SPSS13.0 statistical software is used for statistical analysis. The difference between the two is tested by t test, and it is considered statistically significant when P<0...

Embodiment 3

[0084] Example 3 Inhibition of SENP3 gene expression

[0085] 1. siRNA design and synthesis

[0086] siRNA sequences targeting SENP3:

[0087] siRNA1-SENP3:

[0088] The sense strand is 5'-ACGUUUGAAGGAAUUCGUCCA-3' (SEQ ID NO.7);

[0089] The antisense strand is 5'-GACGAAUUCCUUCAAACGUAU-3' (SEQ ID NO.8),

[0090] siRNA2-SENP3:

[0091] The sense strand is 5'-AACUAUUGAAGAAAUGCACCU-3' (SEQ ID NO.9);

[0092] The antisense strand is 5'-GUGCAUUUCUUCAAUAGUUUC-3'(SEQ ID NO.10),

[0093] siRNA3-SENP3:

[0094] The sense strand is 5'-UAGAUACUUGGCAAUAUGCUU-3' (SEQ ID NO.11);

[0095] The antisense strand is 5'-GCAUAUUGCCAAGUAUCUACA-3' (SEQ ID NO.12)

[0096] Negative control siRNA sequence (siRNA-NC):

[0097] The sense strand is 5'-CGUACGCGGAAUACUUCGA-3' (SEQ ID NO.13);

[0098] The antisense strand is 5'-UCGAAGUAUUCCGCGUACG-3' (SEQ ID NO.14).

[0099] 2. Culture and transfection of esophageal cancer cells

[0100] 2.1 Cell culture

[0101] The esophageal cancer cell line ...

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Abstract

The invention discloses SENP3 gene and use of its expression product as diagnosis and treatment target for esophageal cancer. Whether a subject has esophageal cancer can be determined or whether a subject is under risk of having esophageal cancer can be diagnosed by detecting the contents of SENP3 gene and its expression product in tissues. By studying proliferation of esophageal cancer cells cultured in vitro, it is discovered that expression inhibition for SENP3 gene may inhibit the proliferation of esophageal cancer cells, and by inhibiting functionality of SENP3 protein, it is also possible to inhibit the proliferation of esophageal cancer cells; results of this study show that SENP3 and its expression product are a potential drug target to treat esophageal cancer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use of SENP3 gene in the diagnosis and treatment of esophageal cancer. Background technique [0002] Esophageal cancer (EC) is one of the ten most common malignant tumors in the world (more than 300,000 new cases are diagnosed every year). The prognosis of esophageal cancer is poor, and the five-year survival rate is less than 10%. The incidence rate of digestive system tumors is the second, second only to gastric cancer, accounting for 2% of all malignant tumors, and the morbidity and mortality rates remain high, accounting for the sixth in the death of patients caused by various malignant tumors. Among all patients with esophageal cancer, the proportion of middle-aged and elderly people is relatively high, and they are susceptible. Generally speaking, the incidence rate is low for those younger than 30 years old, and the incidence rate after 30 years old is positively correlate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574A61K45/00A61P35/00
Inventor 田子强温士旺苏鹏徐延昭
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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