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Probe-integrated functional nucleic acid modified electrode for direct electrochemical detection of lead ions

A technology for modifying electrodes and functional nucleic acids, which is applied in the direction of electrochemical variables of materials, measuring devices, scientific instruments, etc., can solve problems such as inapplicability, and achieve the effects of strong functionality, improved detection convenience, and low cost

Inactive Publication Date: 2018-12-18
NANTONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF THE PEOPLES REPUBLIC OF CHINA
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Problems solved by technology

[0003] Since fluorescence detection, ultraviolet detection and electrochemiluminescence detection all require relatively large and expensive detection instruments, these methods are not suitable for some relatively simple workplaces and field operations

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  • Probe-integrated functional nucleic acid modified electrode for direct electrochemical detection of lead ions
  • Probe-integrated functional nucleic acid modified electrode for direct electrochemical detection of lead ions

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Experimental program
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Embodiment Construction

[0018] (1) Preparation of probe-integrated functional nucleic acid-modified electrodes

[0019] The gold nanoparticle-modified electrode was self-assembled in a buffer solution containing 1 μM thiol-modified 17E DNAzyme for 12 hours; the buffer solution was a 20 mM Tris-HCl solution containing 140 mM NaCl and 5 mM KCl at pH 7.5; Take out the electrode and wash it with the buffer solution, then put the electrode into the above buffer solution with 1mM mercaptohexanol for passivation for 2 hours, wash with the same buffer solution to remove unassembled mercaptoethanol; finally transfer the electrode to the buffer containing The 1 μM substrate 17DS solution was assembled for 12 hours; the 3' end of the substrate 17DS was modified with a ferrocene probe molecule; the electrode was washed three times with the above buffer solution to obtain a probe-integrated functional nucleic acid modified electrode.

[0020] (2) Detection of lead ions in lake water samples

[0021] The prepared...

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Abstract

The invention discloses a lead ion direct electrochemical detection method for a probe-integrated functional nucleic acid modified electrode. The probe-integrated functional nucleic acid modified electrode is immersed in a buffering solution of to-be-detected lead ions for 20-30 min, the electrode is taken out, washed and subjected to DPV determination in a 0.1 M NaClO4 electrolyte solution, and the lead ion concentration in a sample is calculated according to the change rate of DPV peak current signals scanned and recorded in the range of 0.1-0.6 V. The method is good in selectivity, sensitive, simple and quick.

Description

technical field [0001] The invention relates to a method for directly electrochemically detecting lead ions with a probe-integrated functional nucleic acid modified electrode. Background technique [0002] Lead ion is a common harmful heavy metal ion, and its monitoring / detection is of great significance to human health and environmental monitoring. In the past few decades, people have developed various methods such as atomic absorption / emission spectroscopy, fluorescence spectroscopy, mass spectrometry, photonic crystals, etc. to monitor / detect lead ions. In recent years, the research team of Lu Yi in the United States obtained the functional nucleic acid 17E DNAzyme sensitive to lead ions through in vitro screening technology. They used 17E DNAzyme as the lead ion recognition element and gold nanoparticles as the sensing element to develop a colorimetric sensor to detect lead. ions (Liu, J.; Lu, Y., Nature Protocols 2006, 1, 246-252.). Wang’s group developed a label-free...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/333G01N27/327G01N27/48
CPCG01N27/26G01N27/327G01N27/3275G01N27/333G01N27/48
Inventor 洪华张爱平施筱迪王海天洪昱王红卫王超颖申晓萍
Owner NANTONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF THE PEOPLES REPUBLIC OF CHINA
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