Method for separating and purifying Schwann cell from peripheral nerves of adult macaque

Abstract

The invention relates to the field of cytobiology, and discloses a method for separating and purifying Schwann cell from peripheral nerves of an adult macaque. The method comprises the following steps of 1. obtaining of nerves and in-vitro initial denaturation: under the non-bacteria condition, obtaining the sural and peroneal nerve sections of the adult macaque, removing epineurium, and adopting a SCCM (sertoli's cell culture medium) initial denaturation culture medium containing composite factors to culture, so as to obtain the sural nerves of the adult macaque; 2. primary culture of cells: mixing NB4 and dispase to digest nerves, centrifuging, resuspending in a culture dish of laminin containing CCM (complete culture medium), and incubating; 3. purification of Schwann cells: after incubating of the cells, adding into dispase, digesting, collecting a cell suspension liquid, and incubating in a purifying culture liquid containing Ara-C; then, purifying, centrifuging, and planting into a growth culture medium containing the SCCM. The method has the advantage that in the initial denaturation process, the cell proliferation factors are added, so that the output of cells is improved, the purification time is shortened, and the Schwann cell of the adult macaque with high purity is obtained.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a method for separating and purifying Schwann cells. Background technique [0002] Schwann cells are the main glial cells in the peripheral nervous system, which play a vital role in the growth, development and regeneration of peripheral nerves after injury. After peripheral nerve injury, Wallerian degeneration and disintegration of axons and myelin occur, and Schwann cells proliferate rapidly and together with macrophages, they phagocytize degenerated cells or debris. Schwann cells dedifferentiate, proliferate, migrate, and form Bǜnger bands in the original endoneurial tubes, providing channels for the distal growth of regenerated axonal buds. At the same time, Schwann cells can secrete certain neurotrophic factors to promote the growth of axons, such as: nerve growth factor, ciliary neurotrophic factor, etc., which play a certain role in peripheral nerve regeneration. Under ideal ...

AI Technical Summary

Problems solved by technology

Direct digestion will greatly reduce the yield and purity of Schwann cells, resulting in the waste of nerve tissue; the pre-denaturation method of ordinary medium is used to treat the peripheral nerves of adult animals, and the acquisition efficiency of Schwann cells is low and time-consuming

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