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A kind of colorectal cancer screening kit

A kit and colorectal cancer technology, applied in instruments, biochemical equipment and methods, analytical materials, etc., can solve the problems of high cost, large number of detection genes, low sensitivity and specificity, etc.

Active Publication Date: 2020-01-17
BIOMERIEUX SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the above detection methods, when the number of detected genes is small, the sensitivity and specificity are low, while when the sensitivity and specificity are high, the number of detected genes is too large and the cost is high

Method used

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  • A kind of colorectal cancer screening kit
  • A kind of colorectal cancer screening kit
  • A kind of colorectal cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Example 1 Sample Collection

[0118] After informed consent, peripheral blood samples were collected from 187 colorectal cancer patients (CRC) confirmed by clinicopathological diagnosis and 173 colonoscopy-negative control patients (CNC) between 2006 and 2010.

[0119] CRC was recruited from the Department of Colorectal Surgery, Shanghai Cancer Center, Fudan University, China, and all patients were staged according to the TNM staging system recommended by the International Union Against Cancer (UICC). None of the patients received preoperative chemotherapy or radiotherapy. Patients with hereditary colorectal cancer or inflammatory bowel disease (Corhn's disease or ulcerative colitis) were excluded from the present invention. CNCs were recruited from Shanghai Community Hospital and passed colonoscopy without any symptoms of colorectal cancer or polyps. The demographic and clinical characteristics of the test samples are shown in Table 1:

[0120] Table 1 Characteristi...

Embodiment 2

[0126] Example 2 Detection of expression levels of 18 genes such as DUSP2

[0127] 1. Experimental method

[0128] (1) Selection of housekeeping genes

[0129] The geometric mean of the expression levels of CSNK1G2, DECR1, and FARP1 was used as the "meta-housekeeping gene" as a correction factor for real-time quantitative PCR data.

[0130] (2) RNA extraction and real-time quantitative PCR detection

[0131] Whole blood draw: Take 2.5ml of peripheral blood from each participant in PAXgene TM Blood RNA tubes (PreAnalytix GmbH, Hombrechtikon, CH), and processed according to the instructions;

[0132] Total RNA extraction: according to the instructions, use PAXgene TM Blood RNA system (PreAnalytix) was used to extract total RNA; the amount of total RNA was detected with a spectrophotometer at an OD value of 260nm, and the RNA6000Nano on an Agilent bioanalyzer Kit (Agilent Technologies, Palo Alto, CA, U.S.A.) was used to detect the quality of total RNA, and the RNA with inte...

Embodiment 3

[0201] Example 3 Correlation between gene expression and colorectal cancer

[0202] 1. Correlation between single gene expression and colorectal cancer

[0203] Detect the expression levels of 18 genes in the blood samples of the 200 participants (wherein 100 CRCs, 100 CNCs) of group 1 in the embodiment 1 respectively according to the method of embodiment 2-relative expression, T test and calculate the control group The ROC curve was established based on the average relative expression level of the tumor group, and the area under the curve (AUC) of the ROC curve was calculated. AUC can reflect the correlation between the gene and colorectal cancer.

[0204] (1) DUSP2 (dual specificity phosphatase 2):

[0205]

[0206] The correlation between the expression level of DUSP2 gene and colorectal cancer is 0.75, and the average expression level difference between the control group and the tumor group is significant, P<0.01, low expression of this gene can significantly increase ...

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Abstract

The invention discloses a colorectal cancer screening kit, which comprises related reagents for detecting expression level of any one or more of 18 genes provided by the invention. The invention also discloses an application of 18 genes in preparation of a colorectal cancer screening reagent. The invention further discloses a detection method of 18 genes, a detection kit and a colorectal cancer screening method. The kit disclosed by the invention can accurately detect whether a sample to be detected gets colorectal cancer, and has a good prospect in clinical application.

Description

[0001] This application is a divisional application of the Chinese patent application No. 201210361023.6 with the application date of September 25, 2012 and the title of the invention is "a colorectal cancer screening kit". technical field [0002] The invention relates to a colorectal cancer screening kit. Background technique [0003] Colorectal cancer is one of the most common malignant tumors. It ranks fifth in the incidence of cancer in the United States, ranks fourth in the incidence of cancer in China, and is the third leading cause of cancer death in Europeans. When colorectal cancer is at an early stage, its cure rate is high. Early screening of colorectal cancer is the key to successful treatment and patient survival, and it is also a major challenge in the field of public health. [0004] Traditional testing methods include fecal occult blood test, sigmoidoscopy, colonoscopy, double-contrast barium meal, and digital rectal examination. The patient compliance of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/574C12N15/11
CPCC12Q1/6886C12Q2600/158G01N33/57446G01N33/57484G01N2800/50
Inventor 叶迅孟夏徐清华刘芳吴非
Owner BIOMERIEUX SA
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